Fermentation of non-digestible raffinose family oligosaccharides and galactomannans by probiotics

Abstract

Due to their prebiotic potential indigestible oligosaccharides became a major focus of research interest. In this study the growth of selected probiotic strains including lactobacilli, bifidobacteria, Lactococcus lactis, Streptococcus salivarius ssp. thermophilus, Pediococcus ssp. and Enterococcus faecium with the, raffinose family oligosaccharides (RFOs) raffinose, stachyose and verbascose and galactomannan from guar bean Cyamopsis tetragonoloba (total guar carbohydrates, oligosaccharides (dp 2-4) and polysaccharides (dp > 5), obtained by size exclusion chromatography) were tested by means of turbidity measurements. RFOs were used by 75% of all strains, with some delay for the trisaccharide raffinose and the tetrasaccharide stachyose and a limited fermentation of the pentasaccharide verbascose. L. reuteri, P. pentosaceus and B. lactis HNO19™ were able to ferment not only raffinose and stachyose but also verbascose. Guar oligosaccharides were fermented by 15 out of 20 strains; P. acidilactici, L. acidophilus, L. rhamnosus GG and B. animalis ssp. lactis BB12 metabolized them comparably well as glucose or galactose. Isolated guar polysaccharides were not fermented whereas total guar carbohydrates were fermented by 7 strains, apparently caused by the oligosaccharide content. The findings of this study may be important for functional food products especially for indigestible oligosaccharides which may cause adverse effects in the gut when not cleaved.

Changes in Intestinal Microbiota and Predicted Metabolic Pathways During Colonic Fermentation of Mango (Mangifera indica L.)—Based Bar Indigestible Fraction

“…However, in this work the unclassified specie of Streptococcus was not particularly plentiful. Similarly, Lactobacillus is a widely reported genus that comprises of several probiotic species that can metabolize raffinose carbohydrate and oligosaccharides [52]. Weissella genus is reported as possessing phosphotransferase systems of β-glucosidase and α-galactosidase genes for metabolizing D-cellobiose and D-raffinose [53], but their metabolic capacity was unobserved.…”

Stachyose Prevents Intestinal Mucosal Injury in the Immunosuppressed Mice

“…[17] Several studies have reported that STA stimulates the growth of Lactobacillus and Bifidobacterium in the intestinal tract, inhibits enteric bacilli, and prevents intestinal damage. [18][19][20] The STA-enriched -Galactooligosaccharides stachyose could modulate the profile of intestinal microbiota and enhance the intestinal mucosal barrier function of fish. [21] Soybean oligosaccharides including STA promote cellular immunity, humoral immunity, nonspecific immunity, and immunoglobulin levels in mice.…”

Pengembangan Soyghurt (Yoghurt Susu Kacang Kedelai) Sebagai Minuman Probiotik Tinggi Isoflavon

“…Untuk menghidrolisis komponen tersebut, diperlukan enzim α-galaktosidase yang dihasilkan oleh BAL 27 . Namun, jenis BAL yang digunakan dalam penelitian ini diketahui memiliki tingkat reaktivitas enzim αgalaktosidase rendah 28 . Tingkat viskositas soyghurt yang semakin tinggi akan menghambat pertumbuhan BAL.…”