Fermentation of polysaccharides by Klebsielleae and other facultative bacilli

Ochuba, Greg U; Riesen, V. Lyle Von

doi:10.1128/aem.39.5.988-992.1980

Cited by 21 publications

(12 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…were also detected by the probe chosen. These can ferment inulin (16) and might benefit from the substrate. Thus, the suppression of possible pathogens (33), another beneficial effect of NDO in pig nutrition, was not observed, which is in line with previous observations (10,27,28).…”

Section: Discussionmentioning

confidence: 99%

“…Bacteria reported to ferment NDO in vitro (Bifidobacterium spp., Lactobacillus spp., Klebsiella spp., Bacteroides spp. (3,15,16) as well as dominant bacteria in the porcine intestine (17) were enumerated by fluorescent in situ hybridization (FISH) with 59-Cy3 labeled 16S or 23S rRNA oligonucleotide probes (Supplemental Table 1). Bacterial cells were fixed and hybridization experiments were carried out as described (18).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Inulin Alters the Intestinal Microbiota and Short-Chain Fatty Acid Concentrations in Growing Pigs Regardless of Their Basal Diet

Loh¹,

Eberhard²,

Brunner³

et al. 2006

The Journal of Nutrition

127

View full text Add to dashboard Cite

Inulin stimulates intestinal bifidobacteria in humans and rodents but its effect in pigs is inconsistent. We assessed the effect of inulin on the intestinal microbiota by fluorescent in situ hybridization in growing pigs (age 9-12 wk). Pigs (n = 64) were assigned to 2 types of basal diets [wheat and barley (WB) or corn and wheat gluten (CG)] with or without 3% inulin (WBI or CGI) for 3 and 6 wk (n = 8/group) to test whether naturally occurring dietary fibers influence the inulin effect. Intestinal organic acids, pH values, and residual inulin were determined. The composition of the microbiota was highly individual. The duration of feeding did not affect any of the variables tested; therefore, data for the 2 periods were pooled. Bifidobacteria were detected in less than half of the pigs. Inulin did not stimulate lactobacilli and bifidobacteria numbers irrespective of the basal diet, although 20-50% of inulin was degraded in the jejunum. The number of pigs with colonic bifidobacteria was higher in those fed diets containing inulin (40 vs. 13%; P < 0.05). Total colonic short-chain fatty acid (SCFA) concentrations were lower in both inulin-fed groups due to reduced acetate (P < 0.05). Proportions of colonic butyrate were higher in pigs fed inulin-supplemented diets (P < 0.05). Colonic pH tended to be lower in the WB groups (WB; 6.6 +/- 0.6), and was higher due to inulin (CGI, 7.1 +/- 0.1; P < 0.05). In conclusion, inulin affected intestinal SCFA and the number of pigs harboring bifidobacteria; this effect was independent of the basal diet.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Inulin Alters the Intestinal Microbiota and Short-Chain Fatty Acid Concentrations in Growing Pigs Regardless of Their Basal Diet

Loh¹,

Eberhard²,

Brunner³

et al. 2006

The Journal of Nutrition

127

View full text Add to dashboard Cite

show abstract

“…As, for example, the facultative anaerobes Klebsiella spp. (Ochuba and von Riesen, 1980), E. coli or streptococci (Rycroft et al, 2001) are able to ferment inulin in vitro one could assume an increase in one or more of these bacterial groups in the proximal part. However, no changes in the level of Enterobacteriaceae due to inulin have been observed.…”

Section: Inulinmentioning

confidence: 99%

ORIGINAL ARTICLE: Impact of inulin and a multispecies probiotic formulation on performance, microbial ecology and concomitant fermentation patterns in newly weaned piglets

Mair

Domig

Schedle

et al. 2010

Journal of Animal Physiology and Animal Nutrition

View full text Add to dashboard Cite

The effect of inulin and a multispecies probiotic formulation on performance and microbial parameters in a 28 days feeding trial with newly weaned piglets was assessed. Forty-eight piglets were allocated to a 2 × 2 factorial experiment involving two levels of inulin supplementation (0% or 0.4%) and two levels of probiotics (0 or 1 × 10(9) CFU/kg as fed, comprising enterococci, lactobacilli and bifidobacteria). In digesta samples obtained at slaughter (stomach, jejunum, ileum and colon), selected bacterial groups were enumerated and lactic acid, short chain fatty acids and ammonia concentrations analysed. The overall performance of piglets was unaffected by treatment. Inulin increased total aerobes in stomach and jejunum (p < 0.05), whereas enterococci declined in colon of the inulin group (p < 0.05). Furthermore decreasing colonic acetic acid (p < 0.01) and increasing lactic acid (p < 0.05) was observed for inulin. Probiotics increased total aerobes (p < 0.05) and enterococci (p < 0.01) in ileum and lactobacilli (p < 0.05), enterococci and gram-negative anaerobes (p < 0.01) in colon. Moreover, dry matter content in stomach and colon was lower and acetic acid in colon increased (p < 0.05). A decrease in ileal pH value was noted symbiotically for both additives. However, several parameters showed no synbiotic, but distinct individual effects of inulin and probiotics. Effects occurred along the entire gastrointestinal tract without restriction to the colon.

show abstract

“…Klebsiellae are gram-negative facultative anaerobes. Of the enteric bacteria, they ferment the broadest range of sugars, including all of the pentoses (23,26). Klebsiellae ferment both hexoses and pentoses by the Embden-Meyerhof pathway to yield pyruvate, which is dissimilated to a coliformtype mix of acidic, neutral, and gaseous products including ethanol, acetate, formate, lactate, butanediol, C02, and H2 * Corresponding author.…”

mentioning

confidence: 99%

Fermentation of d -Xylose to Ethanol by Genetically Modified Klebsiella planticola

Tolan

Finn

1987

Appl Environ Microbiol

View full text Add to dashboard Cite

D-Xylose is a plentiful pentose sugar derived from agricultural or forest residues. Enteric bacteria such as Klebsiella spp. ferment D-xylose to form mixed acids and butanediol in addition to ethanol. Thus the ethanol yield is normally low. Zymomonas spp. and most yeasts are unable to ferment xylose, but they do ferment hexose sugars to ethanol in high yield because they contain pyruvate decarboxylase (EC 4.1.1.1), a key enzyme that is absent from enteric bacteria. This report describes the fermentation of D-xylose by Klebsiella planticola ATCC 33531 bearing multicopy plasmids containing the pdc gene inserted from Zymomonas mobilis. Expression of the gene markedly increased the yield of ethanol to 1.3 mol/mol of xylose, or 25.1 g/liter. Concurrently, there were significant decreases in the yields of formate, acetate, lactate, and butanediol. Transconjugant Klebsiella spp. grew almost as fast as the wild type and tolerated up to 4% ethanol. The plasmid was retained by the cells during at least one batch culture, even in the absence of selective pressure by antibiotics to maintain the plasmid. Ethanol production was 31.6 g/liter from 79.6 g of mixed substrate per liter chosen to simulate hydrolyzed hemicellulose. The physiology of the wild-type of K. planticola is described in more detail than in the original report of its isolation.

show abstract

Fermentation of polysaccharides by Klebsielleae and other facultative bacilli

Cited by 21 publications

References 28 publications

Inulin Alters the Intestinal Microbiota and Short-Chain Fatty Acid Concentrations in Growing Pigs Regardless of Their Basal Diet

Inulin Alters the Intestinal Microbiota and Short-Chain Fatty Acid Concentrations in Growing Pigs Regardless of Their Basal Diet

ORIGINAL ARTICLE: Impact of inulin and a multispecies probiotic formulation on performance, microbial ecology and concomitant fermentation patterns in newly weaned piglets

Fermentation of d -Xylose to Ethanol by Genetically Modified Klebsiella planticola

Contact Info

Product

Resources

About