“…Although several reviews do consider the topic of pre-treatment (Karthic and Shiny, 2012a;Venkata Mohan, 2008;Li and Fang, 2007;Mudhoo et al, 2011;Ntaikou et al, 2010;Karthic and Shiny, 2012b;ValdezVazquez and Poggi-Varaldo, 2009;Yasin et al, 2013;Saady, 2013;De Gioannis et al, 2013;Chen et al, 2013;Kim et al, 2013;Venkata Mohan and Goud, 2012;Wong et al, 2014a;Ruggeri et al, 2015), none of these studies has focussed extensively on both inoculum and substrates pre-treatment. In addition, besides the review by Saady (2013), most of the studies on inoculum pretreatment have investigated only suppression of hydrogenotrophic methanogenesis while neglecting the effects of pretreatment on other bacteria like homoacetogens, propionate producers, LAB and SRB which can negatively affect the biohydrogen production.…”