Fermentative butanol production by clostridia

Lee, Sang Yup; Park, Jin Hwan; Jang, Seh Hee; Nielsen, Lars K.; Kim, Jae Hyun; Jung, Kwang S.

doi:10.1002/bit.22003

Cited by 929 publications

(564 citation statements)

References 132 publications

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“…It is well documented that PhaB results exclusively in (R)-3-hydroxyacids while Hbd results in the (S) stereoisomers 33,48 . This phenomenon was experimentally verified in pathways producing both 3HB 29 and 3HV 30 .…”

Section: Resultsmentioning

confidence: 99%

A platform pathway for production of 3-hydroxyacids provides a biosynthetic route to 3-hydroxy-γ-butyrolactone

et al. 2013

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The replacement of petroleum feedstocks with biomass to produce platform chemicals requires the development of appropriate conversion technologies. 3-Hydroxy-g-butyrolactone has been identified as one such chemical; however, there are no naturally occurring biosynthetic pathways for this molecule or its hydrolyzed form, 3,4-dihydroxybutyric acid. Here we design a novel pathway to produce various chiral 3-hydroxyacids, including 3,4-dihydroxybutyric acid, consisting of enzymes that condense two acyl-CoAs, stereospecifically reduce the resulting b-ketone and hydrolyze the CoA thioester to release the free acid. Acetyl-CoA serves as one substrate for the condensation reaction, whereas the second is produced intracellularly by a pathway enzyme that converts exogenously supplied organic acids. Feeding of butyrate, isobutyrate and glycolate results in the production of 3-hydroxyhexanoate, 3-hydroxy-4-methylvalerate and 3,4-dihydroxybutyric acid þ 3-hydroxy-gbutyrolactone, respectively, molecules with potential uses in applications from materials to medicines. We also unexpectedly observe the condensation reaction resulting in the production of the 2,3-dihydroxybutyric acid isomer, a potential value-added monomer.

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Section: Resultsmentioning

confidence: 99%

A platform pathway for production of 3-hydroxyacids provides a biosynthetic route to 3-hydroxy-γ-butyrolactone

et al. 2013

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“…Butanol, a C 4 alcohol, is a biosynthetic alternative to gasoline. Butanol has an energy density of 29.2 MJ/L, which is comparable to that of gasoline (32 MJ/L), an octane number of 87, and can be mixed with gasoline at any percentage or even completely replace it [6]. Further, butanol's high hydrophobicity may allow the use of existing fuel transportation and storage infrastructure without major modifications.The Clostridium butanol pathway requires 1 mol of glucose and 4 mol of NADH to produce 1 mol of butanol.…”

Section: Heterologous Expression Of the Clostridialmentioning

confidence: 99%

Advanced biofuel production in microbes

Peralta‐Yahya

Keasling

2010

Biotechnology Journal

349

226

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The cost-effective production of biofuels from renewable materials will begin to address energy security and climate change concerns. Ethanol, naturally produced by microorganisms, is currently the major biofuel in the transportation sector. However, its low energy content and incompatibility with existing fuel distribution and storage infrastructure limits its economic use in the future. Advanced biofuels, such as long chain alcohols and isoprenoid-and fatty acid-based biofuels, have physical properties that more closely resemble petroleum-derived fuels, and as such are an attractive alternative for the future supplementation or replacement of petroleum-derived fuels. Here, we review recent developments in the engineering of metabolic pathways for the production of known and potential advanced biofuels by microorganisms. We concentrate on the metabolic engineering of genetically tractable organisms such as Escherichia coli and Saccharomyces cerevisiae for the production of these advanced biofuels.

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“…Recently, this process has been revived because of the potential application of butanol as an alternative biofuel (Lee et al, 2008). Typical batch fermentation process of C acetobutylicum can be divided into two phases, acidogenesis and solventogenesis.…”

Section: Introductionmentioning

confidence: 99%

Discovery of a novel gene involved in autolysis ofClostridiumcells

et al. 2013

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Cell a utolysis plays important physiological roles in the life cycle of clostridial cells. Unders tanding the genetic basis of the autolysis phenomenon of pathogenic Clostridium or solvent producing Clostridium cells might provide new insights into this important species. Genes that might be involved in autolysis of Clostridium acetobutylicum, a model clostridial species, were investigated in this study. Twelve putative autolysin genes were predicted in C. acetobutylicum DSM 1731 genome through bioinformatics analysis. Of these 12 genes, gene SMB_G3117 was selected for testing the in tracellular autolysin activity, growth profi le, viable cell numbers, and cellular morphology. We found that overexpression of SMB_G3117 gene led to earlier ceased growth, signifi cantly increased number of dead cells, and clear electrolucent cavities, while disruption of SMB_G3117 gene exhibited remarkably reduced intracellular autolysin activity. These results indicate that SMB_G3117 is a novel gene involved in cellular autolysis of C. acetobutylicum.

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Fermentative butanol production by clostridia

Cited by 929 publications

References 132 publications

A platform pathway for production of 3-hydroxyacids provides a biosynthetic route to 3-hydroxy-γ-butyrolactone

A platform pathway for production of 3-hydroxyacids provides a biosynthetic route to 3-hydroxy-γ-butyrolactone

Advanced biofuel production in microbes

Discovery of a novel gene involved in autolysis ofClostridiumcells

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