Abstract-1,3-Propanediol (1,3-PD) is an important chemical intermediate in manufacturing polyethers, polyurethanes and polyesters. Production of 1,3-PD by microbial fermentation has attracted increasingly attention of researchers. In this study, deletion mutant of K.pneumoniae HD-1 constructed by knocking out lactate dehydrogenase (LDH) gene in our previous work was cultivated in fermentation medium utilizing glucose as carton source and original strain K.pneumoniae were simultaneously used as the control strain under absolutely the same conditions and their capability of 1,3-PD production were compared. The results showed that an increase of 19.98%, 20%, and 20.19% of 1,3-PD titer, productivity, and yield, respectively were obtained from K.pneumoniae HD-1. Obviously it can be inferred that the ability in the production of 1,3-PD of the K.pneumoniae HD-1was better than that of the K.pneumoniae, therefore it could be a promising strain for 1,3-PD production in the further study of industrialized production.