Fermentative hydrogen production by a new alkaliphilic Clostridium sp. (strain PROH2) isolated from a shallow submarine hydrothermal chimney in Prony Bay, New Caledonia

Mei, Nan; Zergane, Nesrine; Postec, Anne; Erauso, Gaël; Ollier, Angélique; Payri, Claude; Pelletier, Bernard; Fardeau, Marie‐Laure; Ollivier, Bernard; Quéméneur, Marianne

doi:10.1016/j.ijhydene.2014.09.111

Cited by 39 publications

(38 citation statements)

References 54 publications

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“…Therefore, the mixed consortium developed in this study showed dominance of microorganisms which are widely reported as hydrogen producers. Similar studies are reported where BES and pH was used for enrichment of acidogenic mixed culture showed dominance of Clostridium species (Beckers et al, 2015;Mei et al, 2014;Nguyen et al, 2008;Zhang et al, 2006).…”

Section: Microbial Community Analysissupporting

confidence: 85%

Comparative evaluation of the hydrogen production by mixed consortium, synthetic co-culture and pure culture using distillery effluent

Mishra

Roy

Das

2015

Bioresource Technology

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Section: Microbial Community Analysissupporting

confidence: 85%

Comparative evaluation of the hydrogen production by mixed consortium, synthetic co-culture and pure culture using distillery effluent

Mishra

Roy

Das

2015

Bioresource Technology

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“…[] isolated a facultative anaerobic heterotroph, Marinobacter alkaliphilus , from serpentine mud at South Chamorro seamount along the Marianas fore arc that uses nitrate or fumarate as electron acceptor during anaerobic growth and oxygen during aerobic growth. Strain PROH2 of the genus Clostridium [ Mei et al ., ] and Acetoanaerobium pronyense [ Bes et al ., ] was isolated from the internal portions of sections through an active chimney taken from the Needle of Prony (Prony Bay, New Caledonia) and was shown to generate H 2 via fermentation using a variety of carbohydrates and yeast extract. Fermentation and acetogenesis characterize the metabolisms of Alkaliphilus hydrothermalis and Vallitalea pronyensis isolated from another chimney sample from Prony Bay by Ben [ Aissa et al ., , ].…”

Section: Molecular Evidence For Microbial Metabolisms From Hyperalkalmentioning

confidence: 99%

Geochemical bioenergetics during low‐temperature serpentinization: An example from the Samail ophiolite, Sultanate of Oman

Canovas

Hoehler

Shock

2017

J. Geophys. Res. Biogeosci.

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Various classes of microbial and biomolecular evidence from global studies in marine and continental settings are used to identify a set of reactions that appear to support microbial metabolism during serpentinization of ultramafic rocks. Geochemical data from serpentinizing ecosystems in the Samail ophiolite of Oman are used to evaluate the extent of disequilibria that can support this set of microbial metabolisms and to provide a ranking of potential metabolic energy sources in hyperalkaline fluids that are direct products of serpentinization. Results are used to construct hypotheses for how microbial metabolism may be supported in the subsurface for two cases: ecosystems hosted in rocks that have already undergone significant serpentinization and those hosted by deeper, active serpentinization processes.

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“…For example, Acetoanaerobium pronyense, Vallitalea pronyensis and Clostridium sp. PROH2 were isolated from a chimney system in New Caledonia that has a bulk pH of 10.5, though the isolates obtained demonstrated growth in culture at a pH no higher than 9 (Ben Aissa et al, 2014;Mei et al, 2014;Bes et al, 2015;Postec et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

In situ electrochemical enrichment and isolation of a magnetite‐reducing bacterium from a high pH serpentinizing spring

Rowe

Yoshimura

LaRowe

et al. 2017

Environmental Microbiology

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Summary Serpentinization is a geologic process that produces highly reduced, hydrogen‐rich fluids that support microbial communities under high pH conditions. We investigated the activity of microbes capable of extracellular electron transfer in a terrestrial serpentinizing system known as ‘The Cedars’. Measuring current generation with an on‐site two‐electrode system, we observed daily oscillations in current with the current maxima and minima occurring during daylight hours. Distinct members of the microbial community were enriched. Current generation in lab‐scale electrochemical reactors did not oscillate, but was correlated with carbohydrate amendment in Cedars‐specific minimal media. Gammaproteobacteria and Firmicutes were consistently enriched from lab electrochemical systems on δ‐MnO2 and amorphous Fe(OH)3 at pH 11. However, isolation of an electrogenic strain proved difficult as transfer cultures failed to grow after multiple rounds of media transfer. Lowering the bulk pH in the media allowed us to isolate a Firmicutes strain (Paenibacillus sp.). This strain was capable of electrode and mineral reduction (including magnetite) at pH 9. This report provides evidence of the in situ activity of microbes using extracellular substrates as sinks for electrons at The Cedars, but also highlights the potential importance of community dynamics for supporting microbial life through either carbon fixation, and/or moderating pH stress.

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Fermentative hydrogen production by a new alkaliphilic Clostridium sp. (strain PROH2) isolated from a shallow submarine hydrothermal chimney in Prony Bay, New Caledonia

Cited by 39 publications

References 54 publications

Comparative evaluation of the hydrogen production by mixed consortium, synthetic co-culture and pure culture using distillery effluent

Comparative evaluation of the hydrogen production by mixed consortium, synthetic co-culture and pure culture using distillery effluent

Geochemical bioenergetics during low‐temperature serpentinization: An example from the Samail ophiolite, Sultanate of Oman

In situ electrochemical enrichment and isolation of a magnetite‐reducing bacterium from a high pH serpentinizing spring

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