Fermentative Production of Poly(<i>β</i>-<scp>L</scp>-malic acid), a Polyelectrolytic Biopolyester, by<i>Aureobasidium</i>sp

Nagata, Naoki; Nakahara, Tadaatsu; Tabuchi, Takeshi

doi:10.1271/bbb.57.638

Cited by 90 publications

(48 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…PMLA has been reported to be produced by Penicillium cyclopium, [7] Physarum polycephalum [8] and Aureobasidium sp. [9,10] The in vivo production of polyesters can use a variety of carbon sources such as naturally occurring glucose, plant oil and carbon dioxide, however free molecular design of the polyester produced is rather difficult. On the other hand, the "in vitro" synthesis of polyesters can be applicable as part of the biosynthetic route and for the production of an unnatural polyester which is difficult to synthesize in vivo.…”

Section: In Vivo or In Vitromentioning

confidence: 99%

Enzyme-Catalyzed Synthesis and Chemical Recycling of Polyesters

Matsumura¹

2002

Macromol. Biosci.

113

View full text Add to dashboard Cite

This article summarizes the enzyme‐catalyzed synthesis and chemical recycling of biodegradable aliphatic polyesters and poly(carbonate ester)s directed towards establishing green polymer chemistry. Lipase catalyzes the condensation polymerization of a hydroxy acid, diacid with diol, diacid anhydride with oxirane, and polyanhydride with diol, or the ring‐opening polymerization of lactones of small to large rings, and a cyclic diester to produce the corresponding polyesters. Also, lipase catalyzes the condensation polymerization of a dialkyl carbonate with diol, and the ring‐opening polymerization of a cyclic carbonate to produce the corresponding polycarbonates. These polyesters and polycarbonates were selectively degraded by lipase to produce repolymerizable oligomers. These chemical recycling systems using an enzyme will establish a novel methodology for sustainable polymer recycling. Finally, current trends in green polymer production using enzymes are discussed.

show abstract

Section: In Vivo or In Vitromentioning

confidence: 99%

Enzyme-Catalyzed Synthesis and Chemical Recycling of Polyesters

Matsumura¹

2002

Macromol. Biosci.

113

View full text Add to dashboard Cite

show abstract

“…[42] This anionic homopolyester is synthesized by Physarum polycephalum [43,44] and a few lower eukaryotic microorganism such as Penicillium cyclopium [45] and Aureobasidium pullulans. [46,47] So far, no bacteria have been identified which synthesize polymalic acid. The biosynthesis of this polyester is, as yet, only poorly understood, and polymerization seems to occur by a mechanism different from that of the bacterial polyoxoesters.…”

Section: Bacterial Polyoxyestersmentioning

confidence: 99%

Perspectives for Biotechnological Production and Utilization of Biopolymers: Metabolic Engineering of Polyhydroxyalkanoate Biosynthesis Pathways as a Successful Example

Steinbüchel¹

2001

Macromol. Biosci.

323

View full text Add to dashboard Cite

This article provides an overview of biopolymers, classed according to their chemical structures, function and occurrence, the principles of biosynthesis and metabolism in organisms. It will then focus on polyhydroxyalkanoates (PHA) for which technical applications in several areas are currently considered. PHAs represent a complex class of bacterial polyesters consisting of various hydroxyalkanoic acids that are synthesized by bacteria as storage compounds for energy and carbon if a carbon source is present in excess. Poly(3‐hydroxybutyrate), poly(3HB), is just one example. Most other PHAs are only synthesized if pathways exist which mediate between central intermediates of the metabolism or special precursor substrates on one side and coenzyme A thioesters of hydroxyalkanoic acids, which are the substrates of the PHA synthase catalyzing the polymerization, on the other side. During the last decade, basic and applied research have revealed much knowledge about the biochemical and molecular basis of the enzymatic processes for the synthesis of PHAs in microorganisms. The combination of detailed physiological studies, utilization of the overwhelming information provided by the numerous genome sequencing projects, application of recombinant DNA technology, engineering of metabolic pathways or enzymes and molecular breeding techniques applied to plants have provided new perspectives to produce these technically interesting biopolymers by novel or significantly improved biotechnological processes or by agriculture. Some examples for successful in vivo and in vitro engineering of pathways suitable for the synthesis and biotechnological production of PHAs consisting of medium‐chain‐length 3‐hydroxyalkanoic acids and short‐chain‐length hydroxyalkanoic acids will be provided.

show abstract

“…P. polycephalum currently is used to produce PMA for pharmaceutical applications [11]. Nagata et al [15] reported that isolates of the fungus Aureobasidium also produce PMA in high yields. In a small screening study, Liu and Steinbuchel [10] reported that 4 of 8 strains of Aureobasidium produced up to 1.1 g PMA/L prior to optimization.…”

Section: Introductionmentioning

confidence: 99%

“…A. pullulans is well known as the source of the commercial polysaccharide, pullulan [8,19]. The fungus also produces numerous other valuable bioproducts, including industrial enzymes, PMA, and a novel heavy oil [2,7,15]. We recently completed a multilocus molecular phylogeny of A. pullulans [13].…”

Section: Introductionmentioning

confidence: 99%

Poly(β-L-malic acid) production by diverse phylogenetic clades of Aureobasidium pullulans

Manitchotpisit

Skory

Peterson

et al. 2012

Journal of Industrial Microbiology and Biotechnology

View full text Add to dashboard Cite

Poly(β-L-malic acid) (PMA) is a natural biopolyester that has pharmaceutical applications and other potential uses. In this study, we examined PMA production by 56 strains of the fungus Aureobasidium pullulans representing genetically diverse phylogenetic clades. Thirty-six strains were isolated from various locations in Iceland and Thailand. All strains from Iceland belonged to a newly recognized clade 13, while strains from Thailand were distributed among 8 other clades, including a novel clade 14. Thirty of these isolates, along with 26 previously described strains, were examined for PMA production in medium containing 5% glucose. Most strains produced at least 4 g PMA/L, and several strains in clades 9, 11, and 13 made 9-11 g PMA/L. Strains also produced both pullulan and heavy oil, but PMA isolated by differential precipitation in ethanol exhibited up to 72% purity with no more than 12% contamination by pullulan. The molecular weight of PMA from A. pullulans ranged from 5.1 to 7.9 kDa. Results indicate that certain genetic groups of A. pullulans are promising for the production of PMA.

show abstract

Fermentative Production of Poly(β-L-malic acid), a Polyelectrolytic Biopolyester, byAureobasidiumsp

Cited by 90 publications

References 8 publications

Enzyme-Catalyzed Synthesis and Chemical Recycling of Polyesters

Enzyme-Catalyzed Synthesis and Chemical Recycling of Polyesters

Perspectives for Biotechnological Production and Utilization of Biopolymers: Metabolic Engineering of Polyhydroxyalkanoate Biosynthesis Pathways as a Successful Example

Poly(β-L-malic acid) production by diverse phylogenetic clades of Aureobasidium pullulans

Contact Info

Product

Resources

About