A nitrogen mass balance, realized for the lower Oglio River basin (Po River Plain, northern Italy), suggested an elevated impact of agricultural activities in this watershed. Livestock manure, synthetic fertilizers, biological fixation, atmospheric deposition, and wastewater sludge contributed 51,34,12,2, and 1% of total N (TN) . About 34% of the N surplus was exported annually from the basin while the remaining amount (about 26 800 t N year
À1) underwent other unaccounted for processes within the watershed.The relevance of nitrogen removal via denitrification in aquatic compartments within the watershed was evaluated. Denitrification in the secondary drainage network can represent a relevant nitrogen sink due to great linear extension (over 12 500 km), with estimated nitrogen loss up to 8500 t N year À1 . Denitrification in the riverbed and in perifluvial wetlands have the potential to remove only a small fraction of the nitrogen surplus (<3%). Evidence suggests the relevance of groundwater as a site of nitrogen accumulation.
IntroductionIncreased reactive nitrogen (N) input to the biosphere by human activities has resulted in the gradual saturation of the N buffering capacity of terrestrial areas and augmented N loads toward surface and ground waters. Reactive N is now accumulating in the environment at all spatial scales, from local to global [1,2]. Farming activity is one of the primary causes for the current high N loads, as an unbalance between livestock manure production and agricultural lands for spreading causes an available N pool generally exceeding the crop requirements and the soil's metabolic capacity [3,4]. The control of N inputs to aquatic systems is of general public interest due to their known ecosystemic consequences, namely eutrophication [5][6][7] and the potential toxicity risks posed by a high nitrate (NO À 3 ) concentration in water resources [8,9]. Rivers are particularly critical because they link terrestrial and coastal ecosystems, and aggregate stressors occurring at the landscape scale. Human-driven alterations such as channelization, impoundment, water withdrawals, reduction of perifluvial vegetation, and wetlands have enhanced soil erosion and runoff processes, affected the biogeochemistry of riparian and in-stream zones and reduced the efficiency of the river network in mitigating N excess [10][11][12].Worldwide N budgets estimate that, on a long-term basis, up to $75% of the N load generated within catchments is generally retained within the basin and not exported via river discharge [2,13]. The ratio between exported and generated N can be considered an integrated measure of the watershed metabolic capacity toward N. The retained N is the result of several processes, among which some are well studied (i.e., crop uptake) while others are scarcely investigated (i.e., denitrification in lotic ecosystems, N storage in soils, and percolation to groundwater) and represent large unknown terms in N budgets [14]. Even if denitrification is accepted as the major sink of N excess in the...