Fermented ginseng attenuates lipopolysaccharide-induced inflammatory responses by activating the TLR4/MAPK signaling pathway and remediating gut barrier

Fan, Jingjing; Liu, Sitong; Ai, Zhiyi; Chen, Yiying; Wang, Yonghong; Li, Youbao; Li, Xia; Shuhua, Xiao; Wang, Yuhua

doi:10.1039/d0fo02404j

Cited by 30 publications

(18 citation statements)

References 42 publications

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“…Numerous studies have proven that broilers under LPS challenge exhibit compromised growth performance as evidenced by reducing feed intake, weight gain, and feed conversion ratio; disrupted intestinal barrier function; and the negotiation of nutrients away from growth to support various immunizationrelated processes, such as the synthesis of cytokines and various acute proteins (14,20,21). This research confirmed that LPS challenge decreased the body weight of broilers.…”

Section: Discussionsupporting

confidence: 68%

Multi-Omics Revealed the Protective Effects of Rhamnolipids in Lipopolysaccharide Challenged Broilers

Zhang¹,

Shi²,

Chen

et al. 2022

Front. Immunol.

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Rhamnolipid (RL) is a glycolipid biosurfactant and exhibits the following outstanding characteristics: strong antibacterial properties, low toxicity, and high biodegradability. The present research was conducted to explore the protective effects and mechanisms of rhamnolipids as an alternative to antibiotics in LPS (lipopolysaccharide)-challenged broilers. 16S rRNA gene sequencing and metabolomics were used for analyzing the cecal microbial composition and serum metabolites. Dietary antibiotics and RLS supplementation decreased the weight loss rate, enhanced serum immunoglobulin levels, reduced serum diamine oxidase and D-lactate acid concentration, and improved the symptoms of intestinal bleeding and villus height, when broilers were challenged with LPS. The addition of RLS in the diet enhanced serum interleukin-4 and interleukin-10 contents and reduced serum interleukin-6 and tumor necrosis factor-α levels in LPS-challenged broilers compared with the antibiotics group. Spearman’s correlation analysis revealed that RLS may alleviate LPS-induced inflammatory responses through altering the 6-methoxymellein level in broilers. The genus Bacteroides may contribute to the decreased weight loss rate via regulating the serum lysoPC [20:5(5Z,8Z,11Z,14Z,17Z)] secretion. RLS alleviates LPS-induced intestinal injury, enhances the growth and immunity, ameliorates intestinal microflora, and improves serum metabolites in LPS-challenged broilers. RLS exhibited better protective effect than antibiotic supplementation in the diet of LPS-challenged broilers. These findings provide potential regulation strategies and novel insights for RLS enhancing its protective effect in LPS-challenged broilers.

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Section: Discussionsupporting

confidence: 68%

Multi-Omics Revealed the Protective Effects of Rhamnolipids in Lipopolysaccharide Challenged Broilers

Zhang¹,

Shi²,

Chen

et al. 2022

Front. Immunol.

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“…The TJ proteins such as claudin, occludin, and ZO-1 comprise the major intestinal epithelial barrier. The damaged intestinal barrier function causes abnormal expression of TJ proteins, which allows LPS, pathogens, and other harmful substances to pass through the intestinal wall into the blood circulation and increase the activation of the immune system and the inflammatory response [ 14 , 15 , 40 ]. A previous study showed that Lactobacillus rhamnosus Gorbach-Goldin (LGG) played an important role in the strengthening of the mucosal immune barrier, protection against inflammation-induced damage, and maintaining intestinal homeostasis [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

“…Lipopolysaccharide (LPS), a vital component of the E. coli outer membrane, can cause damage to the intestinal epithelial barrier and induce chronic intestinal inflammation [ 12 , 13 ]. LPS contributes to immune defense by interrupting intestinal TJs and inducing systemic inflammatory responses [ 14 , 15 ]. Consequently, LPS was widely used to establish the inflammation correlation model.…”

Section: Introductionmentioning

confidence: 99%

Lactobacillus rhamnosus CY12 Enhances Intestinal Barrier Function by Regulating Tight Junction Protein Expression, Oxidative Stress, and Inflammation Response in Lipopolysaccharide-Induced Caco-2 Cells

Zheng

Ahmad

Yang

et al. 2022

IJMS

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The intestinal barrier is vital for preventing inflammatory bowel disease (IBD). The objectives of this study were to assess whether the Lactobacillus rhamnosus CY12 could alleviate oxidative stress, inflammation, and the disruption of tight junction (TJ) barrier functions induced by lipopolysaccharide (LPS), and therefore to explore the potential underlying molecular mechanisms. Our results showed that LPS-induced Cancer coli-2 (Caco-2) cells significantly increased the levels of reactive oxygen species (ROS), lactate dehydrogenase, inflammatory cytokines interleukin-1β, interleukin-6, interleukin-8, and tumor necrosis factor-α (IL-1β, IL-6, IL-8, and TNF-α), and the cell apoptosis rate while decreasing the levels of TJ proteins occludin, zonula occludens-1 (ZO-1), and claudin and antioxidant enzymes, such as catalase, superoxide dismutase, and glutathione peroxidase(CAT, SOD, and GSH-Px) (p < 0.05). However, Lactobacillus rhamnosus CY12 could relieve cytotoxicity, apoptosis, oxidative stress, and pro-inflammatory cytokine expressions, and also inhibit the Toll-like receptor 4/ nuclear factor kappa-B(TLR4/NF-κB) signaling pathway. Furthermore, the gene expression of antioxidant enzymes, as well as the mRNA and protein expressions of TJ proteins, was improved. Particularly, the concentration of 108 cfu/mL significantly prevented the inflammatory injury induced by LPS in Caco-2 cells (p < 0.05). These findings support a potential application of Lactobacillus rhamnosus CY12 as a probiotic to prevent LPS-induced intestinal injury and treat intestinal barrier dysfunction.

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“…Numerous studies have confirmed that LPS challenge could result in poor compromised growth performance in broilers, as characterized by the depressed feed intake, body weight gain, and feed efficiency. It has been hypothesized that the immune stress induced by LPS injection could redistribute nutrients to synthesize antibody and inflammatory cytokine and elicit immune response, which ultimately lead to the growth retardation [ 18 , 21 , 22 ]. As expected, this study found that LPS injection increased the weight loss rate in broilers response to the established immune stress.…”

Section: Discussionmentioning

confidence: 99%

Dietary Garlic Powder Alleviates Lipopolysaccharide-Induced Inflammatory Response and Oxidative Stress through Regulating the Immunity and Intestinal Barrier Function in Broilers

Zhang

Liu²,

Liu³

et al. 2022

Animals

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Garlic powder (GP) has the outstanding antibacterial, antifungal, antiviral, anti-parasitic and antioxidant characteristics because of its various contained bioactive components, such as alliin, allicin, and polysaccharide, etc. It has been widely used as a native medicine and shown to prevent a variety of diseases. This research was performed to determine the positive effects of GP on growth and intestinal function in lipopolysaccharide (LPS) challenged broilers. A total of 480 one-day-old male Ross 308 broilers of similar initial body weight were randomly divided into four groups with 8 replicates per treatment and 15 chicks each replicate. LPS challenge enhanced the weight loss rate, decreased the immunity and antioxidant capability, increased the intestinal permeability in broilers. When compared with LPS group, broilers fed with GP exhibited improved weight loss rate and jejunum villus height, enhanced ileum antioxidant function, and ameliorated intestinal barrier function. The LPS-challenged broilers in GP group had higher immunity than that of broilers in antibiotics group. GP supplementation could act as a natural alternative to antibiotic additive to alleviate the LPS-induced weight loss rate, inflammatory responses, and oxidative stress in broilers by improving the immunity and intestinal function.

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Fermented ginseng attenuates lipopolysaccharide-induced inflammatory responses by activating the TLR4/MAPK signaling pathway and remediating gut barrier

Abstract: Generally, ginsenosides have the physiological effect of an anti-inflammatory immunity.

Cited by 30 publications

References 42 publications

Multi-Omics Revealed the Protective Effects of Rhamnolipids in Lipopolysaccharide Challenged Broilers

Multi-Omics Revealed the Protective Effects of Rhamnolipids in Lipopolysaccharide Challenged Broilers

Lactobacillus rhamnosus CY12 Enhances Intestinal Barrier Function by Regulating Tight Junction Protein Expression, Oxidative Stress, and Inflammation Response in Lipopolysaccharide-Induced Caco-2 Cells

Dietary Garlic Powder Alleviates Lipopolysaccharide-Induced Inflammatory Response and Oxidative Stress through Regulating the Immunity and Intestinal Barrier Function in Broilers

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