Fermented Soybean Suppresses Visceral Fat Accumulation in Mice

Kushida, Mamoru; Okouchi, Ran; Iwagaki, Yui; Asano, Masaki; Du, Ming; Yamamoto, Kazushi; Tsuduki, Tsuyoshi

doi:10.1002/mnfr.201701054

Cited by 28 publications

(14 citation statements)

References 48 publications

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“…The energy content per 100 g of each test diet was 389, 465, 464, 461, or 459 kcal. Energy was calculated using the modified Atwater method (4 kcal of protein, 9 kcal of lipid, 4 kcal of carbohydrate per g) [13,14].…”

Section: Methodsmentioning

confidence: 99%

“…Intestinal bacterial DNA was extracted from mouse stool samples, and the gene encoding 16S rRNA was amplified by PCR. The composition of intestinal bacteria in feces was examined by meta 16S analysis as described previously [13,25]. Mouse feces was collected daily during the last week of the study period, and DNA was extracted from the recovered feces and pooled for each group.…”

Section: Methodsmentioning

confidence: 99%

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Simultaneous Intake of Euglena gracilis and Vegetables Exerts Synergistic Anti-Obesity and Anti-Inflammatory Effects by Modulating the Gut Microbiota in Diet-Induced Obese Mice

et al. 2019

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We determined whether the anti-obesity effect provided by the consumption of Euglena gracilis (Euglena), which is rich in insoluble dietary fiber, could be enhanced by the co-consumption of vegetables with an abundance of soluble dietary fiber. Nine-week-old male C57BL/6J mice were divided into five groups as follows: group 1 received a normal diet, group 2 received a high-fat diet, and groups 3, 4, and 5 received high fat diets containing 0.3% paramylon, 1.0% Euglena, or 1.0% Euglena plus 0.3% vegetables (barley leaf, kale, and ashitaba), respectively. Mice were fed ad libitum until 18 weeks of age. Euglena intake significantly reduced visceral fat accumulation in obese mice, and co-consumption of vegetables enhanced this effect. Consumption of Euglena with vegetables reduced adipocyte area, suppressed the expression of genes related to fatty acid synthesis, upregulated genes related to adipocyte lipolysis, and suppressed serum markers of inflammation. Notably, we also observed an increase in the fraction of short-chain fatty acid-producing beneficial bacteria, a reduction in harmful bacteria that cause inflammation, and an increase in short-chain fatty acid production. Therefore, the co-consumption of vegetables enhanced the anti-obesity and anti-inflammatory effects of Euglena, likely by modulating the gut microbiota composition.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Simultaneous Intake of Euglena gracilis and Vegetables Exerts Synergistic Anti-Obesity and Anti-Inflammatory Effects by Modulating the Gut Microbiota in Diet-Induced Obese Mice

et al. 2019

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“…The energy content per 100 g of each test diet was 388 or 389 kcal. Energy was calculated using the modified Atwater method (4 kcal of protein, 9 kcal of lipid, 4 kcal of carbohydrate per g) [ 12 , 13 ].…”

Section: Methodsmentioning

confidence: 99%

“…DNA of intestinal bacteria was extracted from mouse stool samples and the gene encoding 16S rRNA was amplified by PCR. The composition of intestinal bacteria in feces was examined by meta 16S analysis as described previously [ 12 , 20 ]. Mouse feces were collected every day during the last week of the study period, DNA was extracted from all recovered feces and pooled for each group to make a stool sample.…”

Section: Methodsmentioning

confidence: 99%

Simultaneous Intake of Euglena Gracilis and Vegetables Synergistically Exerts an Anti-Inflammatory Effect and Attenuates Visceral Fat Accumulation by Affecting Gut Microbiota in Mice

et al. 2018

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We determined whether the benefits provided by the consumption of Euglena gracilis (Euglena), which is a unicellular photosynthesizing green alga and rich in insoluble dietary fiber paramylon, can be enhanced by the co-consumption of vegetables that are rich in soluble dietary fiber. Nine-week-old male C57BL/6J mice were divided into four groups: group 1 received normal diet, whereas groups 2, 3 and 4 received normal diet containing 0.3% paramylon, 1.0% Euglena, or 1.0% Euglena plus 0.3% vegetables (barley leaf, kale and ashitaba), respectively. Mice were fed ad libitum until 18 weeks of age. Euglena intake significantly decreased serum markers of inflammation and co-consumption of vegetables enhanced this reduction. Notably, we observed an increase in the fraction of beneficial bacteria producing short-chain fatty acids, a reduction in harmful bacteria that cause inflammation and an increase in short-chain fatty acid production. Visceral fat accumulation was also reduced. Subsequent analyses showed that co-consumption of Euglena with vegetables reduced adipocyte area, suppressed the expression of genes related to fatty acid synthesis and increased the expression of genes related to adipocyte growth and lipolysis. Therefore, co-consumption of Euglena with vegetables enhanced its anti-inflammatory effect and the inhibitory effect on visceral fat accumulation likely by modulating the composition of gut microbiota.

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“…Soybean ( Glycine max ), which was first domesticated in the Northeastern China around 1100 B.C., is one of the most important crop plants for seed protein and oil content, and for its capacity to fix atmospheric nitrogen through symbioses with soil‐borne microorganisms . It is a rich source of bioactive compounds, some with potentially therapeutic antihyperlipidemic, antihyperglycaemic, antihypertensive, antioxidant, anti‐inflammatory, anticancer, antiobesity, and neuroprotective activities that support the biological plausibility for observational associations. As key active compounds, isoflavones (genistein, daidzein, and glycitein) are changed in the body to phytoestrogens, which are bioactive compounds with mildly estrogenic properties .…”

Section: Introductionmentioning

confidence: 99%

Soy and Isoflavone Consumption and Multiple Health Outcomes: Umbrella Review of Systematic Reviews and Meta‐Analyses of Observational Studies and Randomized Trials in Humans

Zhuang

et al. 2019

Molecular Nutrition Food Res

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Scope To assess the existing evidence of associations between consumption of soy and isoflavone and multiple health outcomes. Methods and results This is an umbrella review of meta‐analyses and systematic reviews of randomized trials and observational studies in humans. 114 Meta‐analyses and systematic reviews are identified with 43 unique outcomes. Soy and isoflavone consumption seems more beneficial than harmful for a series of health outcomes. Beneficial associations are identified for cancers, cardiovascular disease, gynecological, metabolic, musculoskeletal, endocrine, neurological, and renal outcomes, particularly in perimenopausal women. Harmful association is only found for gastric cancer (RR: 1.17, 95% CI: 1.02–1.36) for high intake of miso soup (1–5 cups per day) in male. Conclusion Generally, soy and isoflavone consumption is more beneficial than harmful. The results herein support promoting soy intake as part of a healthy diet. Randomized controlled trials are necessary to confirm this finding.

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Fermented Soybean Suppresses Visceral Fat Accumulation in Mice

Abstract: These results suggest that Natto intake supports maintenance of health.

Cited by 28 publications

References 48 publications

Simultaneous Intake of Euglena gracilis and Vegetables Exerts Synergistic Anti-Obesity and Anti-Inflammatory Effects by Modulating the Gut Microbiota in Diet-Induced Obese Mice

Simultaneous Intake of Euglena gracilis and Vegetables Exerts Synergistic Anti-Obesity and Anti-Inflammatory Effects by Modulating the Gut Microbiota in Diet-Induced Obese Mice

Simultaneous Intake of Euglena Gracilis and Vegetables Synergistically Exerts an Anti-Inflammatory Effect and Attenuates Visceral Fat Accumulation by Affecting Gut Microbiota in Mice

Soy and Isoflavone Consumption and Multiple Health Outcomes: Umbrella Review of Systematic Reviews and Meta‐Analyses of Observational Studies and Randomized Trials in Humans

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