Ferric reducing antioxidant potential (FRAP) of antioxidants using reaction flow chromatography

Jones, Andrew; Pravadali-Cekic, Sercan; Dennis, Gary R.; Bashir, Rashida; Mahon, Peter J.; Shalliker, R. Andrew

doi:10.1016/j.aca.2017.02.032

Cited by 48 publications

(25 citation statements)

References 15 publications

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“…The FRAP assay relies on the ability of antioxidants to reduce iron (III) to iron (II), through the reaction with 2,4,6-tris(2-pyridyl)-s-triazine, forming a violet blue color [54]. The FRAP assay provides a direct estimation of the reductants in a sample and it is based on the ability of the analyte to reduce the Fe 3+ /Fe 2+ couple.…”

Section: Antioxidant Capacities By Spectrophotometric Methodsmentioning

confidence: 99%

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Antifungal, Antitumoral and Antioxidant Potential of the Danube Delta Nymphaea alba Extracts

et al. 2019

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This study aimed to explore for the first time the biological properties such as antifungal, antitumoral and antioxidant of Danube Delta Nymphaea alba (N. alba) leaf and root methanolic extracts. The toxicity studies of N. alba extracts showed no inhibitory effect on wheat seed germination by evaluating the most sensitive physiological parameters (Germination %, Germination index, Vigor index) and using confocal laser scanning microscopy images. The analyzed extracts were found to have high antifungal activity against Candida glabrata with MIC values of 1.717 µg/mL for leaf and 1.935 µg/mL for root. The antitumor activity of the both extracts against A2780/A2780cisR ovarian, LNCaP prostate and MCF-7 breast cancer cells was promising with IC50 values ranging from 23–274 µg/mL for leaf and 18–152 µg/mL for root, and the combination of N. alba extracts with cisplatin showed a synergistic effect (coefficient of drug interaction <1). The antioxidant properties were assessed by β-carotene bleaching, ABTS and FRAP assays and cyclic voltammetry. Quercetin, the most prominent antioxidant, was quantified in very good yields by spectroelectrochemical assay.

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Section: Antioxidant Capacities By Spectrophotometric Methodsmentioning

confidence: 99%

“…A Ferric Reducing Antioxidant Power (FRAP) assay was carried out according to Jones et al [54] with some modifications. Briefly, the FRAP working solution was prepared by mixing 100 mL acetate buffer (250 mM, pH 3.6), 10 mL 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ) solution (10 mM in 40 mM HCl) and 10 mL FeCl3 (20 mM).…”

Section: Frapmentioning

confidence: 99%

Antifungal, Antitumoral and Antioxidant Potential of the Danube Delta Nymphaea alba Extracts

et al. 2019

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“…N , N -dimethyl- p -phenylenediamine (DMPD) [33] and 2,2′-azino- bis (3-ethylbenzothiazolin-6-sulfonic acid (ABTS) [34] cation radical scavenging and ferric reducing antioxidant power (FRAP) [35] were measured using kits from Bioquochem (Llanera-Asturias, Spain). Antiradical activity by 2,2-diphenyl-1-picrylhydrazyl [36] radical scavenging assay, reducing capacity using Folin–Ciocalteau reagent (FCR) [37], and anti-lipoperoxidant activity using inhibition of lipid peroxidation of rat liver microsomes induced by tert -butyl hydroperoxide [38] were determined as described previously with modifications described in detail in our previous research [5,23,39,40,41].…”

Section: Methodsmentioning

confidence: 99%

Sulfated Metabolites of Flavonolignans and 2,3-Dehydroflavonolignans: Preparation and Properties

Valentová

Purchartová

Rydlová

et al. 2018

IJMS

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Silymarin, an extract from milk thistle (Silybum marianum) fruits, is consumed in various food supplements. The metabolism of silymarin flavonolignans in mammals is complex, the exact structure of their metabolites still remains partly unclear and standards are not commercially available. This work is focused on the preparation of sulfated metabolites of silymarin flavonolignans. Sulfated flavonolignans were prepared using aryl sulfotransferase from Desulfitobacterium hafniense and p-nitrophenyl sulfate as a sulfate donor and characterized by high-resolution mass spectrometry (HRMS) and nuclear magnetic resonance (NMR). Their 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and N,N-dimethyl-p-phenylenediamine (DMPD) radical scavenging; ferric (FRAP) and Folin–Ciocalteu reagent (FCR) reducing activity; anti-lipoperoxidant potential; and effect on the nuclear erythroid 2-related factor 2 (Nrf2) signaling pathway were examined. Pure silybin A 20-O-sulfate, silybin B 20-O-sulfate, 2,3-dehydrosilybin-20-O-sulfate, 2,3-dehydrosilybin-7,20-di-O-sulfate, silychristin-19-O-sulfate, 2,3-dehydrosilychristin-19-O-sulfate, and silydianin-19-O-sulfate were prepared and fully characterized. Sulfated 2,3-dehydroderivatives were more active in FCR and FRAP assays than the parent compounds, and remaining sulfates were less active chemoprotectants. The sulfated flavonolignans obtained can be now used as authentic standards for in vivo metabolic experiments and for further research on their biological activity.

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“…Antiradical activity was evaluated spectrophotometrically as the ability of the substances to reduce the DPPH radical as described previously [ 30 ] with minor modifications [ 27 , 28 , 29 ]. DMPD [ 31 ] radical scavenging and FRAP [ 32 ] were measured using kits from Bioquochem (Llanera–Asturias, Spain). The capacity to scavenge the radical cation (ABTS +• ) was evaluated using the Antioxidant Assay Kit (CS0790, Sigma-Aldrich) and expressed as trolox equivalents (TE) from the trolox calibration curve.…”

Section: Methodsmentioning

confidence: 99%

Chemoenzymatic Preparation and Biophysical Properties of Sulfated Quercetin Metabolites

Valentová

Káňová

Meo

et al. 2017

IJMS

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Sulfated quercetin derivatives are important authentic standards for metabolic studies. Quercetin-3′-O-sulfate, quercetin-4′-O-sulfate, and quercetin-3-O-sulfate as well as quercetin-di-O-sulfate mixture (quercetin-7,3′-di-O-sulfate, quercetin-7,4′-di-O-sulfate, and quercetin-3′,4′-di-O-sulfate) were synthetized by arylsulfotransferase from Desulfitobacterium hafniense. Purified monosulfates and disulfates were fully characterized using MS and NMR and tested for their 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+) and N,N-dimethyl-p-phenylenediamine (DMPD) radical scavenging, Folin-Ciocalteau reduction (FCR), ferric reducing antioxidant power (FRAP), and anti-lipoperoxidant activities in rat liver microsomes damaged by tert-butylhydroperoxide. Although, as expected, the sulfated metabolites were usually less active than quercetin, they remained still effective antiradical and reducing agents. Quercetin-3′-O-sulfate was more efficient than quercetin-4′-O-sulfate in DPPH and FCR assays. In contrast, quercetin-4′-O-sulfate was the best ferric reductant and lipoperoxidation inhibitor. The capacity to scavenge ABTS+• and DMPD was comparable for all substances, except for disulfates, which were the most efficient. Quantum calculations and molecular dynamics simulations on membrane models supported rationalization of free radical scavenging and lipid peroxidation inhibition. These results clearly showed that individual metabolites of food bioactives can markedly differ in their biological activity. Therefore, a systematic and thorough investigation of all bioavailable metabolites with respect to native compounds is needed when evaluating food health benefits.

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Ferric reducing antioxidant potential (FRAP) of antioxidants using reaction flow chromatography

Cited by 48 publications

References 15 publications

Antifungal, Antitumoral and Antioxidant Potential of the Danube Delta Nymphaea alba Extracts

Antifungal, Antitumoral and Antioxidant Potential of the Danube Delta Nymphaea alba Extracts

Sulfated Metabolites of Flavonolignans and 2,3-Dehydroflavonolignans: Preparation and Properties

Chemoenzymatic Preparation and Biophysical Properties of Sulfated Quercetin Metabolites

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