Ferrimonas marina sp. nov.

Abstract

A novel Ferrimonas species is described on the basis of phenotypic, chemotaxonomic and phylogenetic studies. Four halophilic organisms were isolated from marine sand and marine macroalgae samples by using high-pH marine agar 2216. An analysis of the nearly complete 16S rRNA gene sequences of these new isolates indicated that they were phylogenetically close (16S rRNA gene sequence similarity >99·5 %, gyrB gene sequence similarity >97·8 %), and were most closely related to Ferrimonas balearica (16S rRNA gene sequence similarity 97·1–97·3 %, gyrB gene sequence similarity 84·4–85·0 %). Chemotaxonomic data (major menaquinone MK7; major fatty acids C16 : 0 and C18 : 1 ω9c) supported the affiliation of the new isolates to the genus Ferrimonas. The results of physiological and biochemical tests allowed phenotypic differentiation of the isolates from F. balearica. It is therefore proposed that the new isolates represent a novel species with the name Ferrimonas marina sp. nov. and type strain A4D-4T (=MBIC06480T=DSM 16917T).

Methyl Glucosyl-3,4-dehydro-apo-8′-lycopenoate, a Novel Antioxidative Glyco-C30-carotenoic Acid Produced by a Marine Bacterium Planococcus maritimus

“…The culture was passed through several rounds of purification to obtain a reproducible homogenous sample. Taxonomical characteristics were analyzed as described in Katsuta et al, 2005. Sequence alignment and similarity search of the 16S rRNA gene sequence were conducted using the Ribosomal database Project [9].…”

“…Cells were harvested after 24 hours cultivation by using MB at 30°C for menaquinone, fatty acid and the guanine and cytosine contents of the genomic DNA analyses. Menaquinones and fatty acids were analyzed as described previously [12]. Chromosomal DNA was isolated and purified according to the method described previously [13].…”

Cerasicoccus maritimus sp. nov. and Cerasicoccus frondis sp. nov., two peptidoglycan-less marine verrucomicrobial species, and description of Verrucomicrobia phyl. nov., nom. rev.

“…Determination of the respiratory quinone system and cellular fatty acid composition were carried out as described previously (Katsuta et al, 2005). DNA was prepared according to the method of Marmur (1961) from cells grown on 1/2 strength R2A agar with 75% artifi cial seawater and the DNA base composition was determined by using the HPLC method of Mesbah et al (1989).…”

Kineosphaera nakaumiensis sp. nov., a novel actinobacterial species of the genus Kineosphaera isolated from sediments in Lake Nakaumi

“…Whole lipid profiles were detected by spraying with molybdatophosphoric acid (5 g molybdatophosphoric acid hydrate in 100 ml ethanol) followed by heating at 150 C (Worliczek et al, 2007). Determination of the respiratory quinone system was carried out as described previously (Katsuta et al, 2005).…”