Ferritin Expression in Rat Hepatocytes and Kupffer Cells after Lead Nitrate Treatment

Yang, Fan; Yamada, Toshiyuki; Shimizu, Takeshi; Nanashima, Naoki; Akita, Miki; Suto, Kohji; Tsuchida, Shigeki

doi:10.1177/0192623308328544

Cited by 14 publications

(9 citation statements)

References 36 publications

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“…The supernatant was used as an extract [36]. Spot density of ferritin band was normalized to the amount of protein, by loading equivalent amount of protein (5 µg), in each lane, for each liver extract analyzed.…”

Section: Methodsmentioning

confidence: 99%

Dietary Iron Concentration May Influence Aging Process by Altering Oxidative Stress in Tissues of Adult Rats

Arruda

Campos

et al. 2013

PLoS ONE

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Iron is an essential element. However, in its free form, iron participates in redox-reactions, leading to the production of free radicals that increase oxidative stress and the risk of damaging processes. Living organisms have an efficient mechanism that regulates iron absorption according to their iron content to protect against oxidative damage. The effects of restricted and enriched-iron diets on oxidative stress and aging biomarkers were investigated. Adult Wistar rats were fed diets containing 10, 35 or 350 mg/kg iron (adult restricted-iron, adult control-iron and adult enriched-iron groups, respectively) for 78 days. Rats aged two months were included as a young control group. Young control group showed higher hemoglobin and hematocrit values, lower levels of iron and lower levels of MDA or carbonyl in the major studied tissues than the adult control group. Restricted-iron diet reduced iron concentrations in skeletal muscle and oxidative damage in the majority of tissues and also increased weight loss. Enriched-iron diet increased hematocrit values, serum iron, gamma-glutamyl transferase, iron concentrations and oxidative stress in the majority of tissues. As expected, young rats showed higher mRNA levels of heart and hepatic L-Ferritin (Ftl) and kidneys SMP30 as well as lower mRNA levels of hepatic Hamp and interleukin-1 beta (Il1b) and also lower levels of liver protein ferritin. Restricted-iron adult rats showed an increase in heart Ftl mRNA and the enriched-iron adult rats showed an increase in liver nuclear factor erythroid derived 2 like 2 (Nfe2l2) and Il1b mRNAs and in gut divalent metal transporter-1 mRNA (Slc11a2) relative to the control adult group. These results suggest that iron supplementation in adult rats may accelerate aging process by increasing oxidative stress while iron restriction may retards it. However, iron restriction may also impair other physiological processes that are not associated with aging.

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Section: Methodsmentioning

confidence: 99%

Dietary Iron Concentration May Influence Aging Process by Altering Oxidative Stress in Tissues of Adult Rats

Arruda

Campos

et al. 2013

PLoS ONE

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“…The 19% greater level of activity of AST in the HePb group was a significant increase. In contrast, the concentrations of bilirubin, LDH 5 …”

Section: Resultsmentioning

confidence: 91%

Function of the liver and bile ducts in humans exposed to lead

et al. 2013

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Lead is very common in the environment, and it is therefore important to characterize its possible adverse health effects. The aim of this study was to evaluate the impact of lead exposure on selected functions of the liver and bile ducts in people who are chronically exposed to the metal because of their occupations. To provide this information, the activity of specific enzymes and the bilirubin concentration were determined in blood serum, and morphological parameters of the liver and bile ducts were evaluated using the ultrasonic imaging method. Healthy male employees of a lead-zinc processing facility (n = 145) who were occupationally exposed to lead were divided into two subgroups as a function of the lead concentrations in blood (PbB): low lead exposure (PbB = 20-35 μg/dl; n = 57) and high lead exposure (PbB = 35-60 μg/dl; n = 88). Human exposure to lead compounds was found to cause liver enlargement and to activate inflammatory reactions with the characteristics of moderate cholestasis within the bile ducts, while no characteristics of necrotic damage of hepatic cells were noted. It seems that lipid peroxidation can be one of the toxic mechanisms of lead which induce moderate cholestasis. The effects depend on the extent of the lead exposure and were greater in subjects with higher exposure levels, particularly subjects with PbB values greater than 35 μg/dl.

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“…This study was performed at 8 weeks of CF administration, because our previous study revealed that preneoplastic foci lacking BE expression developed in sensitive KS‐type rats at this time‐point. ( 12,22 ) α‐SMA, CD68 and CD34 were used as markers for the HSC, Kupffer cell and sinusoidal endothelial cell, respectively, ( 21 ) and the numbers of cells positive for the respective marker were compared between the KS‐ and NC‐type rat livers. Individual staining results are presented in Figure 1.…”

Section: Resultsmentioning

confidence: 99%

“…Apoptotic cell death was examined by the TdT‐mediated dUTP‐biotin nick end‐labeling (TUNEL) analysis, as reported previously. ( 21 )…”

mentioning

confidence: 99%

Decrease of hepatic stellate cells in rats with enhanced sensitivity to clofibrate‐induced hepatocarcinogenesis

Yamana

Shimizu²,

Yang

et al. 2011

Cancer Science

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To examine the possible involvement of nonparenchymal cells in the development of preneoplastic hepatic lesions induced by clofibrate (CF), alterations of these cells were investigated immunohistochemically in glutathione S-transferase M1 gene polymorphic rats (KS and NC types) with different cancer susceptibilities. After CF administration for 8 weeks, α-smooth muscle actin (α-SMA)-positive hepatic stellate cells (HSC) were markedly decreased in sensitive KS-type rats, but not in the NC-type rats. Kupffer cells were decreased with similar extents between them. The sinusoidal endothelial cells were not changed in either type. The other markers for HSC, vimentin and CRBP1, also confirmed the decrease of HSC in the KS type. The decrease of HSC was not observed at 4 weeks of CF administration. Preneoplastic peroxisomal bifunctional enzyme-negative foci were detected in the KS-type rats at 8 weeks of CF administration, but not at 4 weeks. Human HSC were cultured in the presence of clofibric acid and expression of most HSC marker genes, such as vimentin and α-SMA (ACTA2), evaluated by a microarray, was not altered by the treatment, suggesting that HSC loss in the KS-type rats was not due to the direct toxic effect of CF. The expression levels of most HSC marker genes were low in both control and CF-treated rat livers. A possible link between HSC loss and the development of preneoplastic hepatic foci is discussed.

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Ferritin Expression in Rat Hepatocytes and Kupffer Cells after Lead Nitrate Treatment

Cited by 14 publications

References 36 publications

Dietary Iron Concentration May Influence Aging Process by Altering Oxidative Stress in Tissues of Adult Rats

Dietary Iron Concentration May Influence Aging Process by Altering Oxidative Stress in Tissues of Adult Rats

Function of the liver and bile ducts in humans exposed to lead

Decrease of hepatic stellate cells in rats with enhanced sensitivity to clofibrate‐induced hepatocarcinogenesis

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