Ferritin protein imaging and detection by magnetic force microscopy

Hsieh, Chiung-Wen; Zheng, Bin; Hsieh, Shou-Shing

doi:10.1039/b912338e

Cited by 31 publications

(34 citation statements)

References 22 publications

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“…Really, the main contribution to the uncertainty in MFM quantitative analysis results from that in the magnetic properties of the tip. Therefore, although in recent years MFM has been used to quantitatively study the magnetic properties of ferritin and nanometer sized MNPs, 29 , 39 , 41 , 42 in order to definitely assess the magnetic properties of magnetoferritin and other molecules at the nanoscale the value of m ct should be calibrated using a reference sample. Recently, an approach has been proposed in which such a calibration is performed for a fixed value of Δ z using MNPs with traceably determined magnetization dispersed on a Si surface as a MFM reference sample 30 .…”

Section: Resultsmentioning

confidence: 99%

“…Hsieh et al 39 have recently used MFM to visualize ferritin molecules, to detect their iron oxide core and to quantitatively evaluate the corresponding magnetic moment. Martinez et al 40 employed MFM to confirm the magnetic properties of iron oxide core of patterned ferritin molecules after reducing its size from 8 to 2 nm by O 2 plasma etching.…”

Section: A Short Review Of Applicationsmentioning

confidence: 99%

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Magnetic force microscopy

et al. 2014

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Magnetic force microscopy (MFM) is an atomic force microscopy (AFM) based technique in which an AFM tip with a magnetic coating is used to probe local magnetic fields with the typical AFM spatial resolution, thus allowing one to acquire images reflecting the local magnetic properties of the samples at the nanoscale. Being a well established tool for the characterization of magnetic recording media, superconductors and magnetic nanomaterials, MFM is finding constantly increasing application in the study of magnetic properties of materials and systems of biological and biomedical interest. After reviewing these latter applications, three case studies are presented in which MFM is used to characterize: (i) magnetoferritin synthesized using apoferritin as molecular reactor; (ii) magnetic nanoparticles loaded niosomes to be used as nanocarriers for drug delivery; (iii) leukemic cells labeled using folic acid-coated core-shell superparamagnetic nanoparticles in order to exploit the presence of folate receptors on the cell membrane surface. In these examples, MFM data are quantitatively analyzed evidencing the limits of the simple analytical models currently used. Provided that suitable models are used to simulate the MFM response, MFM can be used to evaluate the magnetic momentum of the core of magnetoferritin, the iron entrapment efficiency in single vesicles, or the uptake of magnetic nanoparticles into cells.

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Section: Resultsmentioning

confidence: 99%

Section: A Short Review Of Applicationsmentioning

confidence: 99%

Magnetic force microscopy

et al. 2014

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“…Also, plasma patterning has been used to modify PDMS surfaces to create patterned oxidized regions that exhibit hydrophilic character and can be used for cell patterning [24]. In our published work in this area, plasma patterned PDMS surfaces were used to fix ferritin proteins [25]. In this study, plasma patterned PDMS surfaces were used as an adherent substrate to provide a model system for studying the cellular motion of HCC cells with different histological grades.…”

Section: Introductionmentioning

confidence: 98%

Hepatocarcinoma Single Cell Migration on Micropatterned PDMS Substrates

Zheng¹,

Hsieh

Wu³

et al. 2011

Nano BioMed ENG

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Cell migration influences many normal and pathological processes and is one of key issues addressed in cancer research studies. In this report, a plasma patterned polydimethylsiloxane (PDMS) substrate was used to selectively position hepatocarcinoma cells in order to characterize their migration behavior. We observed that cell mobility was directly related to the differentiation stage of the cells, with poorly-differentiated (SK-Hep-1) cells exhibiting higher mobility that well-differentiated (Hep-G2) cells. We propose that this difference occurs due to a loss of adhesion molecules presented at the apical membranes of the poorly-differentiated SK-Hep-1 cells, thereby reducing their adhesion to the surface. Our results provide new insight into the relationship between carcinoma cell differentiation grade and mobility. Further this experimental process may provide a simple and effective model for universal cell biology studies and applications in microsystems technology.

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“…MFM study of the surface of SPION nanocomposites showed that the distribution of SPIONs in a matrix can create domains in the nanocomposite . The high potential of MFM for biological and medical applications has been shown, for example, by visualization the iron core of ferritin as well as by studying the cellular uptake of SPIONs . Wang et al mapped the uptake of SPIONs into tumor cells and determined the intracellular iron content and spatial distribution of the intracellular iron .…”

Section: Introductionmentioning

confidence: 99%

“…[22] The high potential of MFM for biological and medical applications has been shown, for example, by visualization the iron core of ferritin as well as by studying the cellular uptake of SPIONs. [13,14,[23][24][25][26][27] Wang et al mapped the uptake of SPIONs into tumor cells and determined the intracellular iron content and spatial distribution of the intracellular iron. [26] Passeri et al investigated the use of superparamagnetic core shell nanoparticles for cell labeling in vitro.…”

Section: Introductionmentioning

confidence: 99%

Magnetic Force Microscopy of in a Polymer Matrix Embedded Single Magnetic Nanoparticles

Krivcov¹,

Schneider²,

Junkers

et al. 2018

Physica Status Solidi (a)

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Embedding superparamagnetic iron oxide nanoparticles (SPIONs) in functional thin films is of high interest for nanocomposites as well as for biomedical applications. However, there is still the need for high‐resolution mapping of the hidden nanoparticles. Magnetic force microscopy (MFM) has proved to be a suitable technique to image SPIONs at ambient conditions and provide information about spatial distribution, size, and magnetic behavior in a single pass. This work reports on high‐resolution mapping of magnetite nanoparticles of various sizes embedded in polymer films of various thicknesses. A quantification of the magnetic signals is used to determine the size of the magnetic core of the embedded nanoparticles.

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Ferritin protein imaging and detection by magnetic force microscopy

Abstract: Magnetic force microscopy was used to image and detect ferritin proteins and the strength of the magnetic signal is discussed, revealing a large workable lift height between the magnetic tip and the ferritin sample.

Cited by 31 publications

References 22 publications

Magnetic force microscopy

Magnetic force microscopy

Hepatocarcinoma Single Cell Migration on Micropatterned PDMS Substrates

Magnetic Force Microscopy of in a Polymer Matrix Embedded Single Magnetic Nanoparticles

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