Ferroferric oxide/l-cysteine magnetic nanospheres for capturing histidine-tagged proteins

High‐efficiency Ni2+‐NTA/PAA magnetic beads with specific separation on His‐tagged protein

“…Magnetic nanoparticles have unique advantages in simple operation, fast separation, and high throughput, thus opening a new window for protein purification [10]. To develop functional magnetic nanoparticles for protein purification, many efforts have been made recently [11][12][13][14]. Especially, the key to rapid separation of target proteins is to couple the suitable affinity agents on the surface of magnetic nanoparticles [15].…”

“…Especially, the key to rapid separation of target proteins is to couple the suitable affinity agents on the surface of magnetic nanoparticles [15]. Zou et al [14] successfully synthesised Fe 3 O 4 /Cys-Ni 2+ nanoparticles for rapid enrichment and purification of His-tagged proteins directly from the mixture of lysed cells without pretreatment. Hwang et al [16] synthesised Fe 3 O 4 nanoparticles functionalised with Zn-DPA ligands for specifically enriching phosphoproteins from complex cell and tissue lysates.…”

A porous nano-adsorbent with dual functional groups for selective binding proteins with a low detection limit

“…Evidently, the surfaces of these nanospheres were rough, which may be due to the oriented growth and crystallinity. 27 As shown in Fig. 1b, the obtained SiO 2 -S/NH-Ni sample displays a small porous structure, with about 3.7 nm, in the silica skeleton.…”

“…[23][24][25] With the development of nanotechnology, nanomaterials are used in many elds, such as protein purication, environmental treatment, targeted drugs and so on. [26][27][28] However, the major limitations of current nanomaterial systems are the detection limit and poor regeneration ability, which leads to low purication efficiency. In this study, porous silica with dual functional groups (-SH and -NH 2 groups) was employed as an adsorbent to separate His-tagged proteins.…”

Facile synthesis of sea urchin-like magnetic copper silicate hollow spheres for efficient removal of hemoglobin in human blood

“…Very recently, affinity particle-adsorbents have developed for the separation of His-rich or His-tagged proteins. [8][9][10] Generally, iminodiacetic acid (IDA), tris(carboxymethyl) ethylenediamine (TED) and nitrolotriacetic acid (NTA) can be used as chelating ligands. For example, nickel-nitrolotriacetic acid (Ni-NTA) complex-conjugated Au/Fe 3 O 4 , FePt and silica were applied to separate His-tagged proteins.…”