Ferrostatin‑1 alleviates liver injury via decreasing ferroptosis following ricin toxin poisoning in rat

Lin, Ruijiao; Jia, Zijie; Chen, Hongbing; Xiong, Hongli; Bian, Cunhao; He, Xin; Wei, Bi; Fu, Junfeng; Zhao, Minzhu; Li, Jianbo

doi:10.1016/j.tox.2024.153767

Cited by 3 publications

(1 citation statement)

References 47 publications

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“…At present, RSL3 or erastin is known to induce ferroptosis by inhibiting the activity of glutathione peroxidase 4 (GPX4). , HeLa cells treated with RSL3 (1 μM) or erastin (10 μM), followed by the treatment with SRF-HClO (10 μM) for 0.5 h, showed strong red fluorescent signals from inside the lysosomes with feeble green fluorescent signals (Figure ), indicating that the HClO level increased significantly during the ferroptosis process. However, upon incubating the cells with ferrostatin-1 (Fer-1, a classical ferroptosis inhibitor), the fluorescence intensity of the probe remained basically the same as that of the probe only. , In addition, further validated by flow cytometry, the conclusion was consistent with the above (Figure S8). These results indicate that SRF-HClO has the ability to monitor dynamic HClO during the ferroptosis process.…”

Section: Resultssupporting

confidence: 77%

Ratiometric Fluorescent Probe for Super-Resolution Imaging of Lysosome HClO in Ferroptosis Cells

Zheng,

Peng,

Liu

et al. 2024

Anal. Chem.

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Ferroptosis is an iron-dependent programmed cell death that is characterized by the dysregulation of lipid reactive oxygen species (ROS) production, causing abnormal changes in hypochlorous acid (HClO) levels in lysosomes. Super-resolution imaging can observe the fine structure of the lysosome at the nanometer level; therefore, it can be used to detect lysosome HClO levels during ferroptosis at the suborganelle level. Herein, we utilize a ratiometric fluorescent probe, SRF-HClO, for super-resolution imaging of lysosome HClO. Structured-illumination microscopy (SIM) improves the accuracy of lysosome targeting and enables the probe SRF-HClO to be successfully applied to rapidly monitor the up-regulated lysosome HClO at the nanoscale during inflammation and ferroptosis. Importantly, the probe SRF-HClO can also detect HClO changes in inflammatory and ferroptosis mice and evaluate the inhibitory effect of ferroptosis on mice tumors.

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Section: Resultssupporting

confidence: 77%

Ratiometric Fluorescent Probe for Super-Resolution Imaging of Lysosome HClO in Ferroptosis Cells

Zheng,

Peng,

Liu

et al. 2024

Anal. Chem.

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Discovery of phenazine derivatives as a new class of non-classical ferroptosis inhibitors and efficacy evaluation on a mouse model of liver injury

Wu,

Yang,

You

et al. 2025

European Journal of Medicinal Chemistry

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Syzygium aromaticum Extract Mitigates Doxorubicin-Induced Hepatotoxicity in Male Rats

Alsirhani,

Abu-Almakarem,

Alwaili

et al. 2024

IJMS

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Doxorubicin (DOX), an anticancer drug, is used to treat several types of tumors, but it has detrimental side effects that restrict its therapeutic efficacy. One is the iron-dependent form of ferroptosis, which is characterized by elevated ROS production and iron overload. Syzygium aromaticum has a diverse range of biological and pharmaceutical actions due to their antioxidant properties. This study investigated the effect of S. aromaticum extract (SAE) on hepatotoxicity caused by DOX in rats. Phytochemical analysis was performed to assess compounds in SAE. The ADMETlab 2.0 web server was used to predict the pharmacokinetic properties of the most active components of SAE when DOX was injected into rats. Molecular docking studies were performed using AutoDock Vina. Forty male Sprague Dawley rats were divided into four groups of ten rats each (G1 was a negative control group, G2 was given 1/10 of SAE LD50 by oral gavage (340 mg/kg), G3 was given 4 mg/kg of DOX intraperitoneally (i.p.) once a week for a month, and G4 was administered DOX as in G3 and SAE as in G2). After a month, biochemical and histopathological investigations were performed. Rats given SAE had promising levels of phytochemicals, which could significantly ameliorate DOX-induced hepatotoxicity by restoring biochemical alterations, mitigating ferroptosis, and upregulating the NRF-2–SLC7A-11–GPX-4 signaling pathway. These findings suggest that SAE could potentially alleviate DOX-induced hepatotoxicity in rats.

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Ferrostatin‑1 alleviates liver injury via decreasing ferroptosis following ricin toxin poisoning in rat

Cited by 3 publications

References 47 publications

Ratiometric Fluorescent Probe for Super-Resolution Imaging of Lysosome HClO in Ferroptosis Cells

Ratiometric Fluorescent Probe for Super-Resolution Imaging of Lysosome HClO in Ferroptosis Cells

Discovery of phenazine derivatives as a new class of non-classical ferroptosis inhibitors and efficacy evaluation on a mouse model of liver injury

Syzygium aromaticum Extract Mitigates Doxorubicin-Induced Hepatotoxicity in Male Rats

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