1998
DOI: 10.1016/s1056-8719(98)00025-2
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Ferrous iron-induced luminol chemiluminescence: a method for hydroxyl radical study

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Cited by 95 publications
(10 citation statements)
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“…Hydroxyl radicals were generated by the addition of ferrous iron to the buffer solution (Yıldız & Demiryürek, 1998). Freshly prepared 200 lM FeSO 4 (in 0.9% NaCl) and various concentrations of daylily flower extracts were added to the 0.1 M PBS (pH 7.4) in the stainless steel cell for 60 s. At the 60-s mark, 3 mM luminol was injected into the cell and the chemiluminescence was measured continuously for a further 180 s. The total chemiluminescence intensity was calculated by integrating the area under the curve and subtracting the background level.…”
Section: Scavenging Activity Of Hydroxyl Radical ( å Oh) By Daylily Fmentioning
confidence: 99%
“…Hydroxyl radicals were generated by the addition of ferrous iron to the buffer solution (Yıldız & Demiryürek, 1998). Freshly prepared 200 lM FeSO 4 (in 0.9% NaCl) and various concentrations of daylily flower extracts were added to the 0.1 M PBS (pH 7.4) in the stainless steel cell for 60 s. At the 60-s mark, 3 mM luminol was injected into the cell and the chemiluminescence was measured continuously for a further 180 s. The total chemiluminescence intensity was calculated by integrating the area under the curve and subtracting the background level.…”
Section: Scavenging Activity Of Hydroxyl Radical ( å Oh) By Daylily Fmentioning
confidence: 99%
“…To better determine whether As(III) oxidation occurred in part due to the presence of valence band holes in the presence of light, we carried out experiments that utilized mannitol. Prior studies have shown that mannitol is an efficient scavenger of OH· and oxidative holes [ 46 ]. Figure 5 shows data from an experiment where 20 mM mannitol was added to particular reaction mixtures.…”
Section: Resultsmentioning
confidence: 99%
“…The addition of mannitol to certain reaction solutions was also carried out to supplement the coumarin studies. Prior studies have shown that mannitol is an effective scavenger of OH· and oxidative holes formed during the irradiation of semiconductor materials [ 46 ]. In these particular experiments, 20 mM mannitol was added to specific reaction mixtures of birnessite and As(III) and the effect of the mannitol addition on product formation was investigated under both light and dark conditions.…”
Section: Methodsmentioning
confidence: 99%
“…Hydroxyl radical was generated by addition of ferrous iron into the buffer solution as described by Yıldız and Demiryürek (10). Freshly prepared FeSO 4 (40 nM) was added to the PBS plus luminol (250 μM) mixture and CL was recorded continuously for 3 min.…”
Section: Methodsmentioning
confidence: 99%