Ferruginibacter albus sp. nov., isolated from a mountain soil, and Mucilaginibacter robiniae sp. nov., isolated from a black locust flower, Robinia pseudoacacia

Abstract

Two bacterial strains, designated KIS38-8T and F39-2T, were isolated from a mountain soil sample and a black locust flower (Robinia pseudoacacia) in Republic of Korea, respectively. The phylogenetic tree based on 16S rRNA gene sequences showed that strain KIS38-8T was classified into the genus Ferruginibacter with the highest sequence similarity to Ferruginibacter lapsinanis HU1-HG42T (96.6 %), and strain F39-2T was grouped into the genus Mucilaginibacter with the highest sequence similarity to Mucilaginibacter daejeonensis Jip 10T (97.6 %). Orthologous average nucleotide identity and digital DNA–DNA hybridization values between strain KIS38-8T and closely related Ferruginibacter strains were less than 72 and 19 %, respectively, while those values between strain F39-2T and closely related Mucilaginibacter strains were less than 73 and 21 %, respectively. The DNA G+C contents of strain KIS38-8T and F39-2T were 36.4 and 41.4 mol%, respectively. On the basis of the phenotypic and genotypic evidence, strains KIS38-8T and F39-2T are considered to represent novel species of the genus Ferruginibacter and Mucilaginibacter , respectively, for which the names Ferruginibacter albus sp. nov. (type strain KIS38-8T=KACC 17328T=NBRC 113101T) and Mucilaginibacter robiniae sp. nov. (type strain F39-2T=KACC 19733T=JCM 33062T) have been proposed.

Mucilaginibacter straminoryzae sp. nov., isolated from rice straw used for growing periphyton

“…The Gram-stain reaction was examined using a Gram-stain kit (Becton Dickinson). The temperature range (4,10,17,20,25,30,37,40,45 and 60 °C) and pH range (pH 4.0-12.0, at intervals of 0.5 pH unit) for growth were determined after 7 days by using R2A agar. The pH was adjusted using the following buffer systems: pH 4.0-6.5 (phosphate-citrate), pH 7.0-9.0 (Tris-HCl) and pH 9.5-11.0 (Na 2 HPO 4 -NaOH).…”

“…The hydrolysis of lipid, carboxymethylcellulose, starch and skimmed milk were tested according to the method of Smibert and Krieg [45]. Antibiotic susceptibility of strain RS28 T was determined using the Kirby-Bauer disc diffusion method [46] with antibiotic discs including the following amounts (μg per disc): amikacin (30), amoxicillin (10), cefadroxil (30), cefoperazone (75), ceftazidime (30), ceftriaxone (30), ciprofloxacin (5), erythromycin (15), gentamicin (10), netillin (30), nitrofurantoin (300), norfloxacin (10), penicillin (10), tobramycin (10) and vancomycin (30). Cells were Gram-stain-negative, non-spore-forming, rod-shaped (0.4-0.5 µm wide and 1.5-1.8 µm long), non-flagellated (Fig.…”

“…Ever since that description, this genus has been amended by Urai et al [2], Baik et al [3], Chen et al [4] and Kim et al [5]. At the time of writing, the genus comprises 71 validly named species [6] isolated from a variety of habitats, such as soil [7][8][9], flowers [10], fresh water [3], and plants [11]. The pan-genome analysis of Mucilaginibacter species revealed they have a complex carbohydrate utilization process [12].…”