Fertility after intact ovary transplantation

Xiang, Wu; Chen, Huifang; Yin, Hang; Kim, S. Samuel; Tan, Seang Lin; Gosden, Roger G.

doi:10.1038/415385a

Cited by 211 publications

(111 citation statements)

References 6 publications

Supporting

Mentioning

106

Contrasting

Unclassified

Order By: Relevance

“…Transplantation of the whole ovaries by vascular anastomosis has been performed in experimental animals [11,19,20]. Moreover, fertility in these animal transplantation models was evidenced by the delivery of offspring by the experimental rats and ewes [11,21]. Despite of these encouraging results, however, a dramatic depletion in follicular density was observed in transplanted frozenthawed grafts.…”

Section: Discussionmentioning

confidence: 87%

Effects of cooling rates and ice-seeding temperatures on the cryopreservation of whole ovaries

Zhang

Yan

Cao

et al. 2011

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose The aim of this study was to detect the effects of different cooling rates and different ice-seeding temperatures on the cryopreservation of whole ovaries. Methods Cow whole ovaries were slowly frozen using different protocols with different cooling rates and different ice-seeding temperatures. Follicular viability was assessed using the trypan blue test; the percentage of morphologically normal primordial follicles and the follicular densities of grafts were measured. Results Protocol IIb was most effective protocol. Protocol Ib was more effective than protocol Ia and protocol Ic, and protocol IIIb was more effective than protocol IIIa and protocol IIIc. Conclusions Protocol IIb (the cooling rate was 0.2°C/min, and the ice-seeding temperature was −5°C) was appropriate for slow freezing of cow whole ovaries.

show abstract

Section: Discussionmentioning

confidence: 87%

Effects of cooling rates and ice-seeding temperatures on the cryopreservation of whole ovaries

Zhang

Yan

Cao

et al. 2011

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…In the past few years, attempts at freezing and grafting whole ovaries in animal have yielded encouraging results. The first case of restoration of fertility in rats after whole frozen-thawed ovary transplantation was described by Wang et al in 2002 [13]. They described successful vascular transplantation of frozen-thawed rat ovaries and reproductive tract in 4 out of 7 (57 %) transplants.…”

Section: Discussionmentioning

confidence: 99%

“…Spontaneous pregnancies after transplantation of an intact ovary with microvascular anastomosis have occurred in rat, ewe, and human [12][13][14]. It shows that this technique is promising.…”

Section: Introductionmentioning

confidence: 92%

Optimizing cryoprotectant perfusion conditions for intact ovary: a bovine model

Zhang

Ruan

et al. 2012

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose The aim of this study was to detect the effects of different perfusion pressure and different length of perfusion period on whole ovarian cryopreservation Methods Bovine whole ovaries were vitrified-warmed. The ovaries were divided into the experimental groups according to different perfusion pressure and different length of perfusion period. Follicular viability was assessed using the trypan blue test; the percentage of morphologically normal primordial follicles and the 17-β estradiol level in the culture supernatants were measured. Results When perfusion pressure was 100 mmHg, and the length of perfusion period was 40 min, the viability of ovarian tissues in bovine whole ovarian cryopreservation were higher than other protocols. Conclusion Protocol IIb (the perfusion pressure was 100 mmHg, and the length of perfusion period was 40 min) was appropriate for bovine whole ovarian cryopreservation.

show abstract

“…One of the future approaches to preserve fertility is the transplantation of an intact ovary with vascular anastomosis, allowing immediate revascularization of the ovary. Transplantation of fresh whole ovary with vascular anastomosis has already been successfully performed in rats, 71 rabbits, 72 sheep, 73 dogs, 74 monkeys 75 and humans. 76 The first case of restoration of fertility after whole frozen ovary transplantation was reported by Wang et al in 2002.…”

Section: ) Whole Ovarymentioning

confidence: 99%

Fertility preservation for women with malignancies: current developments of cryopreservation

Chang

Suh

2008

J Gynecol Oncol

View full text Add to dashboard Cite

The preservation of fertility in female cancer survivors has become an important health issue. The different cryopreservation options available for fertility preservation are embryo, oocyte, and ovarian tissue cryopreservation. Oocyte cryopreservation is available for women without partners, but there is a limited experience with this technique and the pregnancy rate is still low. In spite of recent reports of successful birth after autotransplantation of cryopreserved-thawed human ovarian cortical tissues, clinical experience is also limited and this technique remains still experimental. Whole ovary cryopreservation itself poses several challenges. Further researches for establishing optimal cryopreservation and thawing protocols and increasing post-thawing survival, pregnancy, and delivery rates are necessary. In this article, the strategies for fertility preservation in cancer survivors are discussed. The different options and their results are discussed, as well as their indications, efficacy and ethical issues.

show abstract

Fertility after intact ovary transplantation

Cited by 211 publications

References 6 publications

Effects of cooling rates and ice-seeding temperatures on the cryopreservation of whole ovaries

Effects of cooling rates and ice-seeding temperatures on the cryopreservation of whole ovaries

Optimizing cryoprotectant perfusion conditions for intact ovary: a bovine model

Fertility preservation for women with malignancies: current developments of cryopreservation

Contact Info

Product

Resources

About