Fertility and ploidy of gametes of allodiploid and allotriploid loaches produced by diploid Misgurnus anguillicaudatus females and Paramisgurnus dabryanus males

Huang, Songqian; Cao, Xiaojuan; Wang, Weimin; Nasr, Mona

doi:10.1007/s10695-017-0409-5

Cited by 10 publications

(4 citation statements)

References 13 publications

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“…Typically, gametogenesis, which requires normal meiosis, synapsis, and recombination, is blocked in hybrids due to the absence of homologous chromosomes, resulting in the sterility of allodiploid hybrids (Huang et al, 2018). However, the allodiploid GDH hybrids are fertile and produce diploid eggs (Wang et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Formation of the synaptonemal complex in a gynogenetic allodiploid hybrid fish

Wang

Wang²,

Li³

et al. 2023

Front. Genet.

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Introduction: The correct pairing and separation of homologous chromosomes during meiosis is crucial to ensure both genetic stability and genetic diversity within species. In allodiploid organisms, synapsis often fails, leading to sterility. However, a gynogenetic allodiploid hybrid clone line (GDH), derived by crossing red crucian carp (Carassius auratus ♀) and common carp (Cyprinus carpio ♂), stably produces diploid eggs. Because the GDH line carries 100 chromosomes with 50 chromosomes from the red crucian carp (RCC; ♀, 2n = 2x = 100) and 50 chromosomes from the common carp (CC; C. carpio L., ♂, 2n = 2x = 100), it is interesting to study the mechanisms of homologous chromosome pairing during meiosis in GDH individuals.Methods: By using fluorescence in situ hybridization (FISH) with a probe specific to the red crucian carp to label homologous chromosomes, we identified the synaptonemal complex via immunofluorescence assay of synaptonemal complex protein 3 (SCP3).Results: FISH results indicated that, during early ovarian development, the GDH oogonium had two sets of chromosomes with only one set from Carassius auratus, leading to the failure formation of normal bivalents and the subsequently blocking of meiosis. This inhibition lasted at least 5 months. After this long period of inhibition, pairs of germ cells fused, doubling the chromosomes such that the oocyte contained two sets of chromosomes from each parent. After chromosome doubling at 10 months old, homologous chromosomes and the synaptonemal complex were identified.Discussion: Causally, meiosis proceeded normally and eventually formed diploid germ cells. These results further clarify the mechanisms by which meiosis proceeds in hybrids.

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Section: Introductionmentioning

confidence: 99%

Formation of the synaptonemal complex in a gynogenetic allodiploid hybrid fish

Wang

Wang²,

Li³

et al. 2023

Front. Genet.

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“…(Leuciscus alburnoides) and loach (Misgurnusan guillicaudatus) can produce eggs of different sizes and polyploids at the same time (Huang et al 2018;Oshima et al 2005;Alves et al 2001;Zhang et al 1998). The eggs laid by the triploid female brook trout (Salvelinus fontinalis) can be fertilized with normal haploid sperm, but the embryos could only survive till the early stage of hatching (Stillwell and Benfey 1996).…”

Section: Introductionmentioning

confidence: 99%

Bisexual Fertile Triploid Zebrafish (Danio rerio): a Rare Case

Peng

et al. 2020

Mar Biotechnol

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Previous studies have suggested that artificially induced triploid zebrafish are exclusively male-biased. Owing to greatly inhibited gonadal development for the artificially induced triploid fish, they are regarded to be sterile in general. In this article, partially fertile bisexual triploid zebrafish are produced by suppressing extrusion of the second polar body by heat shock. Histological observation confirms that the early gonadal development of these triploid zebrafish is normal. Backcrossing and self-crossing are used to demonstrate that both the female and male triploid zebrafish have partial reproductive ability. Their dynamic of chromosomes during meiosis is revealed from the chromosome preparations of gonads. Examination of the expressed gonadal development-related genes shows some molecular evidence of the normal gonadal development in the triploid zebrafish. Clearly, these fertile bisexual triploid zebrafish can provide a unique system to study sex determination, as well as aneuploidy associated human diseases such as infertility and pregnancy loss.

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“…In recent decades, microstructural observations of gonad development and gametogenesis in triploids have generally shown three situations (i.e. three development types): (1) the structure of gonad tissue was altered in triploid carp (Liu et al 2000(Liu et al , 2002, catfish (Tiwary et al 2000) and catarina scallops (Ruiz-Verdugo et al 2000); (2) spermiogenesis and spermiation were blocked in triploid loach (Suzuki et al 1985;Huang et al 2018), rainbow trout (Sumpter et al 1991) and sea bass (Felip et al 2001); and (3) sperm production was decreased in triploid gilthead seabream (Haffray et al 2005) and yellowtail flounder (Manning et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of testis transcriptomes associated with male infertility in triploid cyprinid fish

Tan

Liu

et al. 2019

Reprod. Fertil. Dev.

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Spermatogenesis involves a series of cellular transformations and thousands of regulated genes. Previously, we showed that the triploid fish (3nBY) cannot produce mature spermatozoa. In the present study, evaluation of the testis microstructure revealed that germ cells of 3nBY could develop into round spermatids, but then degenerated, resulting in male infertility. In this study we comparatively analysed the testis transcriptomes from 3nBY and its diploid parent YB and identified a series of differentially expressed genes (DEGs) that were enriched in the Wnt signalling pathway and the apoptotic and ubiquitin-mediated proteolysis processes in 3nBY. Gene ontology functional analyses revealed that some DEGs in 3nBY were directly associated with the process of gamete generation, development and sperm flagellum assembly. In addition, the expression of a number of genes related to meiosis (Inhibitor Of DNA Binding 2 (ID2), Ovo Like Transcriptional Repressor 1 (OVOL1)), mitochondria (ATP1b (ATPase Na+/K+ Transporting Subunit Beta 1), ATP2a (ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2), ATP5a (ATP Synthase F1 Subunit Alpha), Mitochondrially Encoded Cytochrome C Oxidase I (COX1), NADH Dehydrogenase Subunit 4 (ND4)) and chromatin structure (Histone 1 (H1), Histone 2a (H2A), Histone 2b (H2B), Histone 3 (H3), Histone 4 (H4)) was lower in the testes of 3nBY, whereas the expression of genes encoding ubiquitin (Ubiquitin Conjugating Enzymes (UBEs), Ring Finger Proteins (RNFs)) and apoptosis (CASPs (Caspase 3, Caspase 7,Caspase 8), BCLs (B-Cell Lymphoma 3, B-Cell CLL/Lymphoma 2, B Cell CLL/Lymphoma 10)) proteins involved in spermatid degeneration was higher. These data suggest that the disrupted expression of genes associated with spermatogenesis and the increased expression of mitochondrial ubiquitin, which initiates cell apoptosis, may result in spermatid degeneration in male 3nBY. This study provides information regarding the potential molecular regulatory mechanisms underlying male infertility in polyploid fish.

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Fertility and ploidy of gametes of allodiploid and allotriploid loaches produced by diploid Misgurnus anguillicaudatus females and Paramisgurnus dabryanus males

Cited by 10 publications

References 13 publications

Formation of the synaptonemal complex in a gynogenetic allodiploid hybrid fish

Formation of the synaptonemal complex in a gynogenetic allodiploid hybrid fish

Bisexual Fertile Triploid Zebrafish (Danio rerio): a Rare Case

Comparative analysis of testis transcriptomes associated with male infertility in triploid cyprinid fish

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