Fertility Following CIDR Based Synchronization Regimens in Anoestrous Nili‐Ravi Buffaloes

Abstract

The objective of this study was to compare oestrus expression and fertility rate in used and new controlled internal drug releasing (CIDR) device treated anoestrous buffaloes. Furthermore, to determine the timing of ovulation, and fertility rate in estradiol benzoate (EB) and GnRH-administered CIDR-treated anoestrous Nili-Ravi buffaloes. In experiment 1, buffaloes received either a used CIDR (UCIDR, n = 35) or a new CIDR (NCIDR, n = 36) for 7 day and PGF2α on day 6. Oestrous expression was similar (p > 0.05) between UCIDR (88.5%) and NCIDR (96.6%) buffaloes. The pregnancy rate did not differ (p > 0.05) because of treatment (37.1% in UCIDR vs 36.6% in NCIDR). In experiment 2, buffaloes (n = 55) received CIDR device for 7 days and PGF2α, on day 6 and randomly assigned into three treatment groups: (i) CIDR-EB (n = 17) received EB on day 8, (ii) CIDR-GnRH (n = 18) received GnRH on day 9 and (iii) control (n = 20) received no further treatment. Mean interval from CIDR removal to ovulation in CIDR-EB, CIDR-GnRH and CIDR group were 61.3 ± 0.8, 64.9 ± 1.8 and 65.1 ± 16.7 h, respectively. However, the buffaloes in the CIDR-EB and CIDR-GnRH group had lesser variability in the timing of ovulation compared to control. The pregnancy rate of both CIDR-EB group (58%) and CIDR-GnRH group (61%) were tended to be higher (p < 0.1) than control (30%). In conclusion, compared to NCIDR devices, previously UCIDR devices are equally effective to induce oestrus in anoestrous buffaloes resulting optimal pregnancy rate. Administration of EB and GnRH after CIDR removal results in tighter synchrony (less variability) and improved fertility in anoestrous buffaloes. CIDR based synchronization regimens have great potential in fertility improvement in anoestrous buffaloes.

Effect of oestradiol benzoate on oestrus intensity and pregnancy rate in CIDR treated anoestrus nulliparous and multiparous buffalo

“…In the present study, treatment with OEB did not affect pregnancy rate in these buffalo but the overall pregnancy rate was consistent with results from a previously reported study in Nili-Ravi buffalo (50% compared with 59%) (Naseer et al, 2011). Interestingly, P/AI was considerably less in nulliparous than multiparous buffalo.…”

“…Despite these qualities, some of the main Most of the oestrous synchronization protocols in buffalo are empirically based on those developed for cattle by inducing premature luteolysis using prostaglandins (Kumaratillake et al, 1977;Pathiraja et al, 1979) or prolonging the luteal phase using progestagens (Martinez et al, 1997;Martinez et al, 2000Martinez et al, , 2002 which ultimately resulted in variable fertility. Among many of these oestrous synchronization protocols the use of CIDR device for reproductive management in buffalo is most prevalent, as few previous studies achieved comparable pregnancy rates in anoestrous buffalo during breeding or non-breeding seasons (Murugavel et al, 2009;Ahmad et al, 2010;Naseer et al, 2011;Carvalho et al, 2013;Barlie et al, 2015). The use of the CIDR has resulted in a resumption of oestrous cycles in anovular cows (Rhodes et al, 2002) and Holstein heifers (Yusuf et al, 2010).…”

“…time (mm) 11.9 ± 0.9 10.7 ± 0.5 Ovulatory follicle size (mm) 17.4 ± 0.5 16.3 ± 0.4 Timing of ovulation-hours (range) 72.9 ± 3.6 62.4 ± 1.8 Ovulation rate 64% (7/11) 91% (10/11) Egyptian buffalo using the CIDR with administration of GnRH (Zaabel et al, 2009;Naseer et al, 2011). Furthermore, there was greater oestrous synchrony and greater ovulation rates achieved both in nulliparous and multiparous buffalo after OEB treatment, which could be very conducive to implementation of a FTAI program, thus, eliminating time and labor required for detection of oestrus.…”

“…However, the effectiveness of these oestrous synchronization protocols appears to be limited in buffalo because farmers have to rely on visual methods of oestrous detection, which is not efficient in most of the buffalo herds or commercial farms. To overcome this, administration of OEB after removal of CIDR allows for a timed artificial insemination (TAI) program with optimal pregnancy rates (∼60%) in a limited number of buffalo (Naseer et al, 2011). This approach induced acceptable ovulation rates (∼80%) with greater synchrony of behavioral oestrus (Sales et al, 2012).…”

Relationship between angioarchitecture of the testicular artery and spermiogram parameters in Egyptian buffalo bulls (bubalus bubalis)

“…However, knowledge about the thermoregulatory mechanism of the testes still needs more clarification. Buffalo is blamed for slow reproduction, long calving interval, delayed puberty, poor oestrous expression and seasonality in breeding and calving (Naseer, Ahmad, Singh, & Ahmad, 2011). Moreover, different management systems may affect productive and reproductive performance of the animals such as age at puberty, conception rate, service period and disease problems (Di Palo et al, 2009;Hansson & Öhlmér, 2008).…”