Fertility of a spontaneous hexaploid male Siberian sturgeon, Acipenser baerii

Havelka, Miloš; Hulák, Martin; Ráb, Petr; Rábová, M.; Lieckfeldt, Dietmar; Ludwig, Arne; Rodina, Marek; Gela, David; Pšenička, Martin; Bytyutskyy, Dmytro; Flajšhans, Martin

doi:10.1186/1471-2156-15-5

Cited by 36 publications

(28 citation statements)

References 42 publications

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“…Although 10N males mature normally, the females exhibit delayed maturation and therefore increased time to harvest for caviar (Schreier and Van Eenennaam, 2018). Similar results of matings between individuals with differing ploidy levels has been observed in Siberian sturgeon (Acipenser baerii) (Havelka et al, 2014(Havelka et al, , 2016. Furthermore, spontaneous autopolyploids are poor candidates for release into the wild from conservation hatcheries, which sustain several white sturgeon populations throughout North America, due to negative effects such as delayed reproductive maturation in the F 2 and subsequent generations (Schreier and Van Eenennaam, 2018).…”

Section: Introductionmentioning

confidence: 55%

A comparison of methods for determining ploidy in white sturgeon (Acipenser transmontanus)

et al. 2019

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Release of sturgeon with abnormal ploidy into the wild may result in reduced fitness due to lowered fertility in the F 2 and subsequent generations. Further, there is evidence that ploidy affects reproductive development and caviar yield. Therefore, the ability to accurately characterize the ploidy of white sturgeon (Acipenser transmontanus) is essential for both commercial and conservation aquaculture. This study compares nuclear volume and whole erythrocyte long-axis lengths obtained using Coulter counter and blood smears, respectively, from captive white sturgeon from populations originating in California and Idaho. We examine which method provides the most accurate, time efficient and cost-effective characterization of ploidy in this species. Results from Coulter counter and blood smears were compared to results from flow cytometry, the gold standard for genome size analysis. Previous work suggests that blood smears can distinguish between 8N (diploid) and 12N (triploid) sturgeon, but further analysis is required to see if this method can also be used to identify 10N fish and to provide robust evidence of its utility in 8N and 12N fish across populations. In this study, we demonstrated that the Coulter counter had 100% agreement with flow cytometry in ploidy assignment, while blood smears vary in their accuracy based on population. Blood smears showed a high degree of overlap in erythrocyte long-axis length between 8N and 10N individuals as well as some overlap between 10N and 12N individuals in the California fish, and a high degree of overlap between 8N and 12N individuals in the Idaho fish. Although blood smears are time-intensive and vary in their ploidy assignment accuracy, they are a low-cost technique and as such may have some utility for caviar farms attempting to identify 12N individuals in a small number of broodstock. By comparing the accuracy, efficiency and cost of these three methods, sturgeon farmers and conservation hatcheries will be able to choose the best method for their needs in determining the ploidy of their fish. We determined that Coulter counter is equally accurate to flow cytometry and is also the most time efficient method for ploidy determination in white sturgeon.

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Section: Introductionmentioning

confidence: 55%

A comparison of methods for determining ploidy in white sturgeon (Acipenser transmontanus)

et al. 2019

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“…Also, allopolyploidization and autopolyploidization seem to be a recurring process in sturgeon with observed abnormal ploidy levels in these populations. Moreover, the possible fertility of odd ploidy levels in sturgeon is assumed [28]. Generally in fish, interspecific hybridization of distantly related species causes sterility by complications in the pairing of their chromosomes [29].…”

Section: Problematic Sterilization Of Sturgeon Speciesmentioning

confidence: 99%

Sterilization of sterlet Acipenser ruthenus by using knockdown agent, antisense morpholino oligonucleotide, against dead end gene

et al. 2015

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Sturgeons (chondrostean, acipenseridae) are ancient fish species, widely known for their caviar. Nowadays, most of them are critically endangered. The sterlet (Acipenser ruthenus) is a common Eurasian sturgeon species with a small body size and the fastest reproductive cycle among sturgeons. Such species can be used as a host for surrogate production; application is of value for recovery of critically endangered and huge sturgeon species with an extremely long reproductive cycle. One prerequisite for production of the donor's gametes only is to have a sterile host. Commonly used sterilization techniques in fishes such as triploidization or hybridization do not guarantee sterility in sturgeon. Alternatively, sterilization can be achieved by using a temporary germ cell exclusion-specific gene by a knockdown agent, the antisense morpholino oligonucleotide (MO). The targeted gene for the MO is the dead end gene (dnd) which is a vertebrate-specific gene encoding a RNA-binding protein which is crucial for migration and survival of primordial germ cells (PGCs). For this purpose, a dnd homologue of Russian sturgeon (Agdnd), resulting in the same sequence in the start codon region with isolated fragments of sterlet dnd (Ardnd), was used. Reverse transcription polymerase chain reaction confirmed tissue-specific expression of Ardnd only in the gonads of both sexes. Dnd-MO for depletion of PGCs together with fluorescein isothiocyanate (FITC)-biotin-dextran for PGCs labeling was injected into the vegetal region of one- to four-cell-stage sterlet embryos. In the control groups, only FITC was injected to validate the injection method and labeling of PGCs. After optimization of MO concentration together with volume injection, 250-μM MO was applied for sterilization of sturgeon embryos. Primordial germ cells were detected under a fluorescent stereomicroscope in the genital ridge of the FITC-labeled control group only, whereas no PGCs were present in the body cavities of morphants at 21 days after fertilization. Moreover, the body cavities of MO-treated and nontreated fish were examined by histology and in situ hybridization, showing gonads which had no germ cells in morphants at various stages (60, 150, and 210 days after fertilization). Taken together, these results report the first known and functional method of sturgeon sterilization.

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“…Triploidy can contribute to the production of sterile specimens without the problems related to sexual maturation and in some fish species with a higher growth rate than the diploid ones . However, there are exceptions, such as the triploids of Siberian sturgeon, some of which can be fertile (Havelka et al 2014) or some fish species or hybrids of Acipenseridae where the triploid condition is not associated with an increase in growth (FoppBayat et al 2007). On the other hand, producing gynogenetic individuals (both haploid or diploid) can help elucidate the basis of the sex determinism, to assign molecular markers (microsatellites loci and/or AFLPs) to linkage groups, to determine the distance to the centromeres of these markers, and even to produce allfemale or all-male populations (Piferrer et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Chromosomal manipulation in Senegalese sole (Solea senegalensis Kaup, 1858): induction of triploidy and gynogenesis

et al. 2014

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In this study we have developed protocols for induced triploidy and gynogenesis of Senegalese sole (Solea senegalensis), a promising flatfish species for marine aquaculture, in order to: 1) identify the sex-determination mechanism; and 2) to improve its production by generating a) sterile fish, avoiding problems related with sexual maturation, and b) all-female stocks, of higher growth rate. Triploidy was induced by means of a cold shock. Gynogenesis was induced by activating eggs with UV-irradiated sperm, and to prompt diploid gynogenesis, a cold-shock step was also used. Ploidy of putative triploid larvae and gynogenetic embryos were determined by means of karyotyping and microsatellite analysis. Haploid gynogenetic embryos showed the typical "haploid syndrome". As expected, triploid and gynogenetic groups showed lower fertilization, hatching, and survival rates than in the diploid control group. Survival rate, calculated 49 days after hatching, for haploid and diploid gynogenetic groups was similar to those observed in other fish species (0% and 62.5%, respectively), whereas triploids showed worse values (45%). Sex was determined macroscopically and by histological procedures, revealing that all the diploid gynogenetic individuals were females. In conclusion, we have successfully applied chromosomal-manipulation techniques in the flatfish species Senegalese sole in order to produce triploid, haploid, and diploid gynogenetic progenies.

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Fertility of a spontaneous hexaploid male Siberian sturgeon, Acipenser baerii

Cited by 36 publications

References 42 publications

A comparison of methods for determining ploidy in white sturgeon (Acipenser transmontanus)

A comparison of methods for determining ploidy in white sturgeon (Acipenser transmontanus)

Sterilization of sterlet Acipenser ruthenus by using knockdown agent, antisense morpholino oligonucleotide, against dead end gene

Chromosomal manipulation in Senegalese sole (Solea senegalensis Kaup, 1858): induction of triploidy and gynogenesis

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