Fertility of boar semen cryopreserved in extender supplemented with butylated hydroxytoluene

Trzcińska, Monika; Bryła, Magdalena; Gajda, B.; Gogol, P.

doi:10.1016/j.theriogenology.2014.07.045

Cited by 37 publications

(29 citation statements)

References 34 publications

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“…The detection of the apoptotic-like changes in spermatozoa is a very important parameter for evaluating the fertilizing ability of frozen-thawed spermatozoa. As previously reported by our laboratory (Trzcińska et al 2015) the low value of post-thaw spermatozoa with apoptotic-like changes results in higher insemination success.…”

Section: Discussionsupporting

confidence: 82%

“…A way to protect the spermatozoa from oxidative damage could be the addition of antioxidants to the freezing extenders. Previous studies have shown that supplementation of antioxidants in extenders for both chilled and frozen-thawed semen, such as butylated hydroxytoluen (BHT), SOD, CAT, and GSH has improved boar semen quality (Roca et al 2005, Yeste et al 2014, Trzcińska et al 2015.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, apoptotic-like changes during cryopreservation of buffalo (Kadirvel et al 2012), equine (Ortega-Ferrusola et al 2008) and boar sperm (Trzcińska et al 2015) have been reported. The presence of a population of spermatozoa with apoptotic-like changes could explain the reduced life span of the surviving population after freezing and thawing.…”

Section: Introductionmentioning

confidence: 99%

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Apoptotic-like changes of boar spermatozoa in freezing media supplemented with different antioxidants

Trzcińska¹,

Bryła²

2015

Polish Journal of Veterinary Sciences

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This study evaluated the effect of supplementing the freezing extender with exogenous antioxidants on apoptotic-like changes in post-thaw boar spermatozoa. A total of 36 ejaculates were resuspended in standard lactose-egg yolk-glycerol extender supplemented with antioxidant to final concentrations of 0 (as control), 2.5mM GSH (group I), 5.0 mM GSH (group II), 150 IU/mL SOD (group III), 300 IU/mL SOD (group IV), 200 IU/mL CAT (group V), 400 IU/mL CAT (group VI), 150 IU/mL SOD+200 IU/mL CAT (group VII), 300 IU/mL SOD+400 IU/mL CAT (group VIII). Sperm motility and apoptotic-like changes were determined before and after freeze-thawing. The various markers of apoptotic-like changes were measured: plasma membrane permeability by YO-PRO-1/PI assay, phosphatidylserine (PS) translocation across the plasma membrane using fluorescein-labeled Annexin-V, mitochondrial transmembrane potential detected by JC-1, and DNA fragmentation evaluated by TUNEL assay. The highest percentage of progressive motile sperm was noticed in group II (PM% 64.2±15.4) compared with control (PM% 36.8±5.5). The supplementation of 400 IU/mL CAT (group VI) revealed significant (P<0.01) reduction of apoptotic-like changes (YO-PRO-1 + /PI -: 13.1±7.5%, AnV + /PI -: 9.9±4.1%) in frozen-thawed spermatozoa compared with extender supplemented with 200 IU/mL CAT (group V). Irrespective of the concentration used, SOD and CAT in combination (group VII and group VIII) significantly (P<0.01) improved post-thaw sperm survival compared with the control. Evaluation by TUNEL assay revealed that cryopreservation and thawing did not induce DNA fragmentation in boar spermatozoa.

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Section: Discussionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Apoptotic-like changes of boar spermatozoa in freezing media supplemented with different antioxidants

Trzcińska¹,

Bryła²

2015

Polish Journal of Veterinary Sciences

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“…The addition to the freezing extender in different concentrations and antioxidant associations, superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GSH), such as 5 mM of GSH and SOD in combination with CAT (150 + 200 or 300 + 400 UI/ml) as well as 400 IU/ml of CAT, managed to reduce apoptotic‐like changes in the sperm. Similarly, the use of 1.0 mM of butylated hydroxytoluene (BHT) improves its fertilization capacity (Trzcińska, Bryła, Gajda, & Gogol, ). The addition of 2 mM of GSH maintained the acrosin activity in well‐frozen ejaculates, this enzyme positively correlating with reproductive output (Estrada et al, ).…”

Section: Seminal Plasmamentioning

confidence: 99%

Preservation of boar semen: An update

Pezo¹,

Romero

Zambrano³

et al. 2019

Reprod Domestic Animals

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Contents In the pork industry, artificial insemination and the storage of boar semen in liquid at 17°C are routinely applied to optimize the ejaculate and bring about rapid genetic changes that are reflected in the animal protein. Although the results are satisfactory, they are below what occurs with natural mating. It is currently possible to preserve boar semen with storage at 17°C and slow freezing, since to date there is only one study on vitrification, with negative results applicable only in the case of implementing an intracytoplasmic sperm injection. In both methods and due to the sensitivity of boar sperm to osmotic and temperature changes, there is a loss in the quality of the initial sample; however, slow freezing in boar semen has greater deleterious effects on the sample that are reflected in the pregnancy rates and number of live births. Therefore, only 1% of all inseminations are done with frozen semen. The aim of this review is to provide advances and results of studies conducted on the preservation of boar semen, delving more deeply into the critical points that each of the preservation techniques presents, including bacterial contamination, extender components, temperature, ice nucleation, use of additives in extenders and the main deleterious effects on sperm quality.

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“…) or butylated hydroxytoluene (Trzcińska et al. ) in the freezing media have had dramatic effects for protecting sperm in vitro , and this effect remains when used for insemination. Excellent fertility (>90% farrowing rate and 13.0 total born) was obtained with glutathione addition when using a total of three billion frozen–thawed sperm in a double IUI.…”

Section: Cryopreserved Sperm Additivesmentioning

confidence: 99%

The Fertility of Frozen Boar Sperm When used for Artificial Insemination

Knox

2015

Reprod Domestic Animals

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Contents One of the limits to practical use of frozen boar sperm involves the lowered fertility when used for artificial insemination. Years of studies have shown that 5–6 billion sperm (approximately 3 billion viable) used in single or multiple inseminations results in pregnancy rates most often between 60 and 70% and with litter sizes between nine and 10 pigs. Yet today, it is not uncommon for studies to report pregnancy rates from 70 to 85% and litter sizes with 11–12 pigs. While global statements about the incidence and reasons for higher fertility are not conclusive, incremental fertility improvements appear independently associated with use of a minimum number of viable sperm (1–2 billion), insemination timing that increases the probability that sperm will be present close to ovulation for groups of females, selection for boar sperm survival following cryopreservation, and modification of the freeze and thaw conditions using additives to protect sperm from oxidative damage. Studies show that techniques such as intrauterine and deep uterine insemination can provide an opportunity to reduce sperm numbers and that control of time of ovulation in groups of females can reduce the need for multiple inseminations and improve the chance for AI close to ovulation. However, optimal and consistent fertility with cryopreserved boar sperm may require a multifaceted approach that includes boar selection and screening, strategic use of additives during the freezing and thawing process, post‐thaw evaluation of sperm and adjustments in sperm numbers for AI, assessment of female fertility and ovulation induction for single insemination. These sequenced procedures should be developed and incorporated into a quality control system for improved fertility when using minimal numbers of cryopreserved boar sperm.

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Fertility of boar semen cryopreserved in extender supplemented with butylated hydroxytoluene

Cited by 37 publications

References 34 publications

Apoptotic-like changes of boar spermatozoa in freezing media supplemented with different antioxidants

Apoptotic-like changes of boar spermatozoa in freezing media supplemented with different antioxidants

Preservation of boar semen: An update

The Fertility of Frozen Boar Sperm When used for Artificial Insemination

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