Fertility of Friesian cows after insemination on the second, third and fourth days of low milk progesterone concentrations

Foulkes, J.A.; Goodey, R. G.

doi:10.1136/vr.122.6.135

Cited by 11 publications

(6 citation statements)

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“…Similarly, it is not known whether the day of insemination (the day after the low progesterone concentrations are first detected) is the second or third day of low progesterone. However, as it has been demonstrated that conception rates fall abruptly to insemination on the fourth day of low progesterone (Foulkes & Goodey 1988), insemination cannot be delayed further. In the current trials, the higher rate of double-inseminations resulted in an overall increase of c. 0-4 inseminations/cow.…”

Section: Discussionmentioning

confidence: 99%

Strategic milk progesterone testing for the detection of silent oestrus and anovulation in dairy cows

Williams¹,

McLeod²

1992

J. Agric. Sci.

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S U M M A R YDairy cows were inseminated on the basis of oestrus detection alone (Control, n = 206) or in conjunction with milk progesterone profiles (Experimental, n = 204). Milk samples were taken at 4-5 day intervals to determine oestrous cyclicity and on alternate days (at strategic times) to determine the time of ovulation. Progesterone profiles were consulted routinely as an aid to oestrus detection, and also to identify and aid diagnosis of cows with reproductive disorders. Cycling cows not observed in oestrus were inseminated on the basis of their progesterone profile alone. Animals showing oestrus within 2 days of a progesterone-timed insemination were inseminated again. In Experiments 1 and 2, inseminations were given from Day 42 post partum, but AI was delayed (mean time of 68 ± 11 days after calving) in Experiment 3.The median interval from calving to first insemination was not significantly different between Experimental and Control groups, but the time of conception was earlier (P < 005) and the median calving interval shorter (P < 005) in progesterone-monitored cows in Expts 1 and 3. In addition, the percentage of cows served within 21 days of the start of the service period was greater (P < 005) in Experimental groups in Expts 1 and 2. This was reflected by an increase in the proportion (P < 005) of cows pregnant by 85 and 100 days after calving. The frequency with which the interservice interval equated to the length of the oestrous cycle was increased (P < 001) by progesterone testing (84-1 % and 53-9% in Experimental and Control cows, respectively). Conception rates did not differ significantly between groups, but the number of double inseminations (P < 0001) and thus the mean number of inseminations/conception (P < 001) were greater in progesterone-monitored cows. Conception rate was lower (P < 005) in cows that failed to show oestrus, and to the first insemination in double-inseminated cows.Progesterone testing reduced (by 44-66%, P < 001) the number of cows presented for veterinary examination and the mean time of presentation (P < 005), and also influenced the diagnosis made.

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Section: Discussionmentioning

confidence: 99%

Strategic milk progesterone testing for the detection of silent oestrus and anovulation in dairy cows

Williams¹,

McLeod²

1992

J. Agric. Sci.

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“…(9) On the other hand, pregnancy rate was high in dairy cows with P4 concentrations >5 ng/mL. (10) In China, there are no independent commercial kits for the measurement of milk P4 concentrations in dairy farms. In addition, the imported ELISA kits cannot be widely used in China because of their high cost.…”

Section: Introductionmentioning

confidence: 97%

Development and Application of an ELISA Kit for the Detection of Milk Progesterone in Dairy Cows

Xu²,

Xia³

et al. 2014

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

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Progesterone (P4) is a steroid gonadal hormone that is mainly produced from the corpus luteum and placenta and has various biological functions, especially reproductive regulation. It is important to establish a specific and sensitive P4 enzyme-linked immunosorbent assay (ELISA) for the study of ovary activity and reproductive disorders. Therefore, we prepared a monoclonal antibody (MAb) in a completed antigen (11α-OH-P4-HS-OVA). Based on the MAb and our previously prepared completed antigen, a highly specific and sensitive ELISA was developed. In the present study, a competitive ELISA for the determination of P4 was described in dairy cow milk. It was found that P4 concentration in milk samples from five pregnant cows was significantly higher than that from five estrus cows. The diagnosis rate for pregnancy and non-pregnancy in 54 dairy cows were 93.3% and 95.8%, respectively, at 19 to 23 days after pregnancy by detecting milk P4 concentration. In summary, the developed ELISA is a potential tool for P4 research and offers an alternative, simple, rapid technique for detecting P4, especially in future large clinical investigations on pregnancy identification and reproductive disorders in dairy farms in China.

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“…This is the period of 12-24 h before ovulation when cattle display standing to be mounted behaviour. Ideally artificial insemination should be performed approximately 12 h after the onset of standing oestrous (Brandt et al 2010;Foulkes and Goodey 1988). The onset of oestrous is indicated by a rapid fall in the concentration of milk progesterone, to below 2-5 ng/mL (Bulman 1979;Sauer et al 1986).…”

Section: Introductionmentioning

confidence: 99%

Detection of progesterone in bovine milk using an electrochemical immunosensor

Zhang

Liu

et al. 2013

Int J of Dairy Tech

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To improve the detection of ovulation and early diagnosis of pregnancy on dairy farms, a disposable electrochemical immunosensor was developed using indirect competitive immunoassays and screen‐printed carbon electrodes. After optimisation of the assay conditions, the linear range was from 0.16 to 50 ng/mL. The limit of detection was 0.16 ng/mL. There was no cross‐reactivity with testosterone, 17‐β‐estradiol and estriol. The intra‐assay coefficient of variation was less than 13% and interassay coefficient of variation was < 9%. The percentage recovery was from 95.8% to 115.7%. Stability lasts 4 days at 4 °C. The results of preliminary application trials indicate that the method could have practical potential.

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Fertility of Friesian cows after insemination on the second, third and fourth days of low milk progesterone concentrations

Cited by 11 publications

References 0 publications

Strategic milk progesterone testing for the detection of silent oestrus and anovulation in dairy cows

Strategic milk progesterone testing for the detection of silent oestrus and anovulation in dairy cows

Development and Application of an ELISA Kit for the Detection of Milk Progesterone in Dairy Cows

Detection of progesterone in bovine milk using an electrochemical immunosensor

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