Fertility of Frozen and Unfrozen Ram Semen

First, N. L.; Sevinge, A.; Henneman, H. A.

doi:10.2527/jas1961.20179x

Cited by 11 publications

(5 citation statements)

References 4 publications

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“…2, Table 2), and in the latter case, more advantage was gained from performing a second insemination when the first had been given early, rather than towards the middle of oestrus (Table 3). It should be noted, however, that the results reported here conflict with those of other workers who have failed to obtain a fertility response of similar magnitude to double insemi¬ nation with frozen ram semen (First, Sevinge & Henneman, 1961;Lopatko, 1963;Salamon, 1967).…”

Section: Discussioncontrasting

confidence: 99%

Fertility of Ram Spermatozoa Frozen by the Pellet Method

Salamon¹,

Lightfoot²

1970

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A series of five experiments was conducted to examine ewe fertility following insemination with ram spermatozoa frozen by the pellet method.With thawed semen of low concentration and relatively poor quality, fertility was very poor with an overall non-return rate of 5 -6% (269 ewes). Following insemination with thawed semen of low and high sperm concentration (0-5 versus 1·5 109 motile cells/ml) the proportions of ewes lambing were 23-7 and 43-8% respectively. There was no difference in the proportion of ewes lambing when the inseminate volume was reduced from 0-3 to 0-1 ml (Exp. 2, dilute semen) or from 0-15 to 0 5 ml (Exp. 3, concentrated semen). Two inseminations gave higher fertility than a single insemination (Exp. 2, 38-8% versus 22-6%; Exp. 3, 53-0% versus 39-7%) but the magnitude of the response varied according to both the time of insemination and the concentration and volume of the inseminate.High doses of oxytocin (5 or 10 i.u., i.m.) depressed the proportion of ewes lambing and relaxin (100 to 12,500 guinea-pig units/ewe) did not affect the depth to which the inseminating pipette could be inserted into the cervix.Overall lambing results (percentage of ewes lambing) for Exps 2, 3, 4 and 5 were 30-5% (266 ewes), 46-3% (231), 49-3% (69) and 27-5% (291) respectively, whereas results for the best treatment combinations in these experiments were 61-9%, 56-7%, 64-7% and 40-4%.

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Section: Discussioncontrasting

confidence: 99%

Fertility of Ram Spermatozoa Frozen by the Pellet Method

Salamon¹,

Lightfoot²

1970

Reproduction

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“…Blackshaw (1960) in one experiment found it advantageous to equilibrate ram spermatozoa for 18 hr before freezing, but in a second experiment equilibration had no effect. First, Henneman, and Magee (1959) found no difference between equilibration times of 1 and 18 hr. With bull semen, Martin (1961) found that storage for 6 hr at 5°C in the presence or absence of glycerol is required before freezing.…”

Section: Discussionmentioning

confidence: 71%

“…In vitro studies (Blackshaw 1960) indicated that milk may replace egg yolk-citrate as a diluent for freezing, but this substitution does not improve fertility (First, Sevinge, and Henneman 1961).…”

Section: Introductionmentioning

confidence: 99%

The Use of Dimethyl Sulphoxide, Glycerol, and Reconstituted Skim Milk for the Preservation of Ram Spermatozoa II. The Influence of Diluent Composition and Processing Time During Freezing to ?79°C with Dimethyl Sulphoxide or Glycerol or Both Compounds

Rc¹

1965

Aust. Jnl. Of Bio. Sci.

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SummaryReconstituted skim milk diluents containing dimethyl sulphoxide or glycerol or both substances were used to deep-freeze ra.m spermatozoa to -79°C. Very poor revival (less than 10%) followed freezing in dimethyl sulphoxide. Small improvements in revival were obtained by adding 2% (w/v) fructose to the diluent and shortening the equilibration time to 10 min before freezing. The inclusion of 1 . 5 % (v/v) dimethyl sulphoxide in a diluent containing 7% (vjv) glycerol improved revival.Hypertonic diluents were better than isotonic diluents for freezing ram spermatozoa. Replacement of the milk solids by fructose, while the tonicity of the freezing diluent remained constant, did not. significantly affect reviva.l. Spermatozoa revived equally well after storage for 5 hr at 5°C before freezing either in the presence of glycerol (equilibrated spermatozoa) or its absence (aged spermatozoa).

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“…There are no comparisons of the fertility of spermatozoa frozen in milk or yolk-citrate. However, only ap¬ proximately 5% of ewes lambed after insemination with semen deep-frozen in yolk-citrate (Emmens & Blackshaw, 1955), whilst when heated homogenized milk was used (First, Sevinge & Henneman, 1961), 23% of 135 ewes lambed after insemination with frozen semen in an experiment, but in another experi¬ ment no lambs were born after ninety-four ewes were inseminated (i.e. an overall lambing rate of 14%).…”

Section: Introductionmentioning

confidence: 97%

Deep-Freezing Ram Spermatozoa: The Effects of Milk, Yolk-Citrate and Synthetic Diluents Containing Sugar

Jones¹,

Martin²

1965

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In a factorial experiment skim milk, egg-yolk-citrate and synthetic diluents composed of fructose or lactose, sodium chloride and phosphate buffer containing 3\m=.\0%w/v of a lyophilized preparation of non-dialysable solids from (cow) milk were used as diluents for deepfreezing ram spermatozoa and for incubating spermatozoa at 37\ s=deg\C after thawing. All samples of semen were diluted forty-fold before freezing. Egg-yolk-citrate was inferior to skim milk as a diluent for freezing spermatozoa and for incubating spermatozoa after thawing. Of the two synthetic diluents, lactose synthetic was better for freezing spermatozoa whilst fructose synthetic was better for incubating spermatozoa after thawing. Only spermatozoa frozen in the lactose synthetic diluent and resuspended after thawing in the fructose synthetic survived incubation at 37\s=deg\ C for 2 hr as well as spermatozoa frozen and incubated in skim milk diluents.Two factorial experiments compared egg-yolk-citrate and skim milk as diluents for freezing semen diluted from ten-to forty-fold. At ten-fold dilution, revival was much the same after freezing in milk or egg-yolk-citrate. A twenty-or forty-fold dilution was better than a tenfold dilution in milk, but revival was depressed at these higher dilution rates after freezing in egg-yolk-citrate. When semen was frozen at a tenfold dilution it was advantageous to resuspend spermatozoa in a diluent free of glycerol after thawing. A diluent based on Krebs\p=m-\Henseleit\p=m-\ Ringer solution containing 0\m=.\5%w/v of non-dialysable milk solids was better for incubating spermatozoa after thawing than egg-yolk-citrate or milk.A period of 5 hr equilibration at 5\ s=deg\ C before freezing was better than 30 min equilibration.

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Fertility of Frozen and Unfrozen Ram Semen

Cited by 11 publications

References 4 publications

Fertility of Ram Spermatozoa Frozen by the Pellet Method

Fertility of Ram Spermatozoa Frozen by the Pellet Method

The Use of Dimethyl Sulphoxide, Glycerol, and Reconstituted Skim Milk for the Preservation of Ram Spermatozoa II. The Influence of Diluent Composition and Processing Time During Freezing to ?79°C with Dimethyl Sulphoxide or Glycerol or Both Compounds

Deep-Freezing Ram Spermatozoa: The Effects of Milk, Yolk-Citrate and Synthetic Diluents Containing Sugar

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