Fertility of Frozen-Thawed Boar Semen

Maxweli, WMC; Salamon, S.

doi:10.1071/bi9790237

Cited by 6 publications

(1 citation statement)

References 10 publications

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“…Previous studies have also not found differences between temperatures of CPA addition on sperm quality (Maxwell and Salamon, 1979; Cochran, 1984). However, it has been reported in several species that the temperature of the addition of the CPAs can affect post‐thaw sperm quality (Salisbury et al, 1978; Parks and Lynch, 1992; Sieme et al, 1998; McGonagle et al, 2002).…”

Section: Discussionmentioning

confidence: 86%

Influence of Various Permeating Cryoprotectants on Freezability of Iberian Red Deer (Cervus elaphus hispanicus) Epididymal Spermatozoa: Effects of Concentration and Temperature of Addition

Fernández-Santos

Esteso

Montoro

et al. 2006

Journal of Andrology

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With the aim of finding an ideal cryoprotectant (CPA) in a suitable concentration for red deer epididymal spermatozoa cryopreservation, we evaluated the effects of the 3 most commonly used CPAs, glycerol (GLY), ethylene glycol (EG), and propylene glycol (PG), on sperm cryoresistance. The aim of Experiment 1 was to evaluate the influence of 3 different final concentrations (3%, 6%, and 12%) of each CPA on sperm freezability. Sperm samples were diluted to a final sperm concentration of ,400 6 10 6 spermatozoa/ mL with a Tris-citrate-fructose-EY extender (TCF) prior to freezing. Sperm cryosurvival was judged in vitro by microscopic assessments of individual sperm motility (SMI), viability, and plasma membrane (by means of the HOS test) and acrosome (NAR) integrities. Thawed samples were incubated at 37uC for 2 hours in the freezing medium. At the end of this incubation period, sperm suspensions were again assessed. Our results showed that 12% of any CPA was toxic to red deer epididymal spermatozoa membrane integrity (P , .05). Moreover, regardless of the level of CPA, results indicated that the cryoprotective effects on red deer epididymal spermatozoa of the 3 CPAs after thawing are in the following sequence: GLY . EG . PG (higher symbols mean P , .001). Furthermore, our results also showed an improvement in sperm parameters when the TCF diluent contained 6% of GLY. In Experiment 2 extenders were prepared using GLY 6%. This experiment was designed to investigate the effect of 2 different temperatures of GLY addition 222uC (ambient temperature) and 5uC2 on sperm freezability. Our results showed a differential response (P , .05) of motility (SMI) to temperature of GLY addition before freezing, the best being 22uC (81.94 6 2.4% vs 72.38 6 2.4%). Although there were no statistically significant differences (P . .05) between the 2 temperatures of GLY addition after thawing in terms of sperm quality, after 2 hours of incubation, results tended to be better when CPAs were added at 22uC. In conclusion, our work showed the efficacy of a TCF diluent with 6% of GLY and its addition at 22uC, as an alternative to the more common 3%-4% of GLY and addition at 5uC, in red deer semen freezing protocols.

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Section: Discussionmentioning

confidence: 86%

Influence of Various Permeating Cryoprotectants on Freezability of Iberian Red Deer (Cervus elaphus hispanicus) Epididymal Spermatozoa: Effects of Concentration and Temperature of Addition

Fernández-Santos

Esteso

Montoro

et al. 2006

Journal of Andrology

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Adrenocortical function in rats adapted to the experimental environment during passive avoidance learning

Kulikova¹,

Kassil²

1982

Bull Exp Biol Med

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Alterations of the daily rhythms of HPT axis induced by chronic unpredicted mild stress in rats

Guo

Liu

et al. 2014

Endocrine

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The relationship between thyroid function and depression has long been recognized. Patients with thyroid disorders are more prone to develop depressive symptoms and conversely depression may be accompanied by various subtle thyroid abnormalities. However, the daily rhythm alteration of the functions of the hypothalamus pituitary thyroid axis (HPT) is uncertain. In the present study, we investigated the effects of chronic unpredictable mild stress (CUMS) on the daily rhythm alterations of triiodothyronine (T3), thyroxine (T4), and Thyroid Stimulating Hormone (TSH) in the plasma. We found that CUMS led to depressive-like behavior and the daily rhythm of T3, T4, and TSH in the plasma being disturbed, as well the plasma levels of T3 and T4 decreased compared to control group. Our findings indicate that CUMS not only induce hypofunction of HPT axis but also the disturbance of daily rhythm of PHT axis in rats.

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Fertility of Frozen-Thawed Boar Semen

Cited by 6 publications

References 10 publications

Influence of Various Permeating Cryoprotectants on Freezability of Iberian Red Deer (Cervus elaphus hispanicus) Epididymal Spermatozoa: Effects of Concentration and Temperature of Addition

Influence of Various Permeating Cryoprotectants on Freezability of Iberian Red Deer (Cervus elaphus hispanicus) Epididymal Spermatozoa: Effects of Concentration and Temperature of Addition

Adrenocortical function in rats adapted to the experimental environment during passive avoidance learning

Alterations of the daily rhythms of HPT axis induced by chronic unpredicted mild stress in rats

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