Fertility of Hydatid Cysts and Viability of Protoscoleces in Slaughtered Animals in Qazvin, Iran

Shahnazi, Mojtaba; Jafari, Anya; Javadi, Maryam; Saraei, Mehrzad

doi:10.5539/jas.v5n1p141

Cited by 6 publications

(4 citation statements)

References 19 publications

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“…All body organs and tissues are considered to be exposed to hydatid cysts infection except the hair, nails and teeth (12). Also the results disagreed with several results of different regions that indicated by(13) in Iran which recorded the prevalence rate of infection 38.46% were found in liver and 61.54% in lung out of Ninety five sheep cysts, also (14) in Morocco who found the lung was more infected than liver .…”

contrasting

confidence: 84%

Molecular detection of Hydatid cyst isolated from Goats in AL-Qadisiyia province by Polymerase Chain Reaction

Alkhaled¹

2015

Kufa Jou. Vete. Med. Sci.

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The present study was conducted during the period from August 2013 to April 2014 in AL-Qadisiyia province to detect the infection of hydatidiosis in Goat using Molecular and conventional methods. One hundred and seventy nine Goat were examined at slaughters in Aldewanyia abattoir. Caprinehydatid cyst were removed from infected liver and lung after visceral inspection of these organs then investigated the viability of protoscolices by staining with eosin stain then examining under light microscope of 40x.The results showed 45 hydatid cyst were found from 179 goat , the prevalence rate of infection (25.13%) . 20 cyst were found fertile only whereas 25 cyst were sterile. Regarding to infection site in organs, out of 45 hydatid cyst collected , 28(62.22%) cyst were isolated from liver whereas 17 (37.77%) isolated from lung with significant differences at p<0.05. Out of 45 hydatid cyst samples were taken from infected goats, and examined by conventional PCR , the PCR product demonstrated an expected fragment of 440 bp in the length , 10samples were given positive cases , 7(25%) samples from liver whereas only 3(12%) from lung samples gave positive PCR result, with statistically significant difference (P<0.05) between two organs.

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contrasting

confidence: 84%

Molecular detection of Hydatid cyst isolated from Goats in AL-Qadisiyia province by Polymerase Chain Reaction

Alkhaled¹

2015

Kufa Jou. Vete. Med. Sci.

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“…The supernatant was removed and 100 μL of the protoscoleces' sediment was obtained for counting; where 20 μL was smeared and the parasites (protoscoleces and brood capsules) were enu-merated and logged as the parasite number per each 100 μL sediment. The process was repeated 2 -3 times to assure the average number (Shahnazi et al, 2013;Hajihossein et al, 2015). After the average protoscoleces counting, one drop from the sediments of the CE sand (protoscoleces; brood capsules, and shreds of the germinal layer) was inspected microscopically as wet unstained mounts to determine the existence of protoscoleces or hooks.…”

Section: Characterization and Classification Of Cystic Echinococcosismentioning

confidence: 99%

Autoimmunity in human CE: Correlative with the fertility status of the CE cyst

et al. 2022

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Summary Cystic echinococcosis is speculated to exert several immune-evasion strategies involving autoimmune-phenomena. We evaluated the hypothesizes that the prevalence of autoantibodies increases in the sera of CE patients that may evidence the association between the parasite and autoimmune diseases. Sera from 63 subjects at distinct types of CE cyst fertility were investigated for antinuclear antibodies (ANA), and anti-CCP antibodies. Plasma levels and cellular production of IL-17A cytokine were specifically defined as being assumed to prime for autoimmunity. Healthy-controls were age and gender-matched to test sera. ANA expressions inside the surgically removed metacestode and adventitial layer were also assayed. Out of 63 patients, 35 % had fertile highly viable cysts (group-1), 41 % had fertile low viable cysts (group-2) and 24 % had non-fertile cysts (group-3). A four-fold increase in ANA sera-levels was detected in group-1 compared with their controls (p-value 0.001) while anti-CCP levels were of insignificant differences. In group-2 and group-3, no significant differences were detected between ANA and anti-CCP sera-levels in CE patients and their controls. IL-17A sera-levels in group-1 and group- 2 were significantly higher than their healthy-controls while being of insignificant differences in group-3, p-value= 0.300. No association was detected between sera-levels of IL-17A and ANA as well as anti-CCP antibodies. Interestingly, relative IL-17A cellular expression associated positive ANA deposition in the parasite cells and adventitial layer. Collectively, based on the parasite fertility, IL-17A and ANA seemed to be involved in the host immune defenses against CE. There is no association between CE and anti-CCP antibodies.

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“…The sediment of each cyst was observed under a light microscope at 40x magnification. Cysts with no protoscoleces were considered to be sterile cysts (23). After being examined, the sediment was preserved in 70% ethyl alcohol for further use.…”

Section: Checking the Fertility Of Cystsmentioning

confidence: 99%

Prevalence and molecular studies on Echinococcus equinus isolated from necropsied donkeys

Desouky¹,

Helmy²,

Sorour³

et al. 2017

IJVS

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In the present study, forty donkeys of different ages and sexes at Giza Zoo, Egypt were investigated between October 2015 and September 2016 for the presence of hydatidosis disease. Hydatid cysts were detected in the livers of 10% of the examined donkeys and these cysts had a fertility rate 100%. Female donkeys were infected with cysts more than males and all infected donkeys were old aged with no cases of infection were detected in young or adult donkeys. Using molecular tools, the DNA extracted from cysts that had been isolated was subjected to PCR amplification, using synthesized oligonucleotide primers, and these were constructed to target the 299 bp within the (ND2) gene, which is considered to be specific for the Echinococcus equinus genotype. The sequenced PCR products showed homology to E.equinus (G4 or horse strain genotype). These results can be used in future to pursue the epidemiological status of the causative strain of hydatidosis in equines at the study area.

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Fertility of Hydatid Cysts and Viability of Protoscoleces in Slaughtered Animals in Qazvin, Iran

Cited by 6 publications

References 19 publications

Molecular detection of Hydatid cyst isolated from Goats in AL-Qadisiyia province by Polymerase Chain Reaction

Molecular detection of Hydatid cyst isolated from Goats in AL-Qadisiyia province by Polymerase Chain Reaction

Autoimmunity in human CE: Correlative with the fertility status of the CE cyst

Prevalence and molecular studies on Echinococcus equinus isolated from necropsied donkeys

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