Fertility preservation in women with cancer: update and perspectives

Marinho, Ricardo Mello; Rodrigues, Jhenifer Kliemchen; Lamaita, Rívia Mara; Cota, Ana Márcia de Miranda; Wainstein, Alberto Julius Alves; Drummond, Ana Paula; Wainstein, Lage; Parreiras, Fernanda Cardoso; Silva, Ana Luisa Menezes; Caetano, João Pedro Junqueira

doi:10.5935/2238-3182.20130078

Cited by 1 publication

(4 citation statements)

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“…[1][2][3][4][5][6] Studies have shown that freezing ovarian tissue fragments may preserve the gametes and also the ability to produce hormones. 2,7,8 The collection procedure can be readily programmed at any phase of the cycle, and the patient's exposure to hormone treatment is not required. 9 When the patient is allowed to conceive, thawed fragments could be transplanted again so that the pregnancy may occur spontaneously or after performing assisted reproduction (AR) techniques.…”

Section: Introductionmentioning

confidence: 99%

“…Studies show that retransplantation is a promising technique, since 60 births have been reported to date. 10,11 Apart from the non-standardization of ovarian tissue cryopreservation techniques, there are problems such as the local ischemia that occurs after retransplantation, 7 which currently limit the results.…”

Section: Introductionmentioning

confidence: 99%

“…Studies have shown that freezing ovarian tissue fragments may preserve the gametes and also the ability to produce hormones. 2 7 8 The collection procedure can be readily programmed at any phase of the cycle, and the patient's exposure to hormone treatment is not required. 9 …”

Section: Introductionmentioning

confidence: 99%

“…Apart from the non-standardization of ovarian tissue cryopreservation techniques, there are problems such as the local ischemia that occurs after retransplantation, 7 which currently limit the results.…”

Section: Introductionmentioning

confidence: 99%

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Follicle Viability after Vitrification of Bovine Ovarian Tissue

Guedes

Rodrigues

Campos

et al. 2017

Rev Bras Ginecol Obstet

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Keywords► in vitro follicular maturation ► fertility preservation ► vitrification AbstractPurpose The present study aimed to evaluate the impact of vitrification on the viability of follicles using a three-dimensional (3D) in vitro culture. Methods Bovine ovarian tissue samples (n ¼ 5) obtained from slaughterhouses were utilized. The cortex was cut into small fragments of 2 Â 3 Â 0.5 mm using a tissue slicer. From these fragments, secondary follicles were first isolated by mechanical and enzymatic methods, then encapsulated in alginate gel and individually cultured for 20 days. Additional fragments of the same ovarian tissue were vitrified in a solution containing 25% glycerol and 25% ethylene glycol. After warming, the follicles underwent the same follicular isolation process that was performed for the fresh follicles. Results A total of 61 follicles were isolated, 51 from fresh ovarian tissue, and 10 from vitrified tissue. After the culture, the vitrified and fresh follicles showed 20% and 43.1% survival rates respectively (p ¼ 0.290), with no significant differences. At the end of the culture, there were no significant differences in follicular diameter between the vitrified (422.93 AE 85.05 µm) and fresh (412.99 AE 102.55 µm) groups (p ¼ 0.725). Fresh follicles showed higher mean rate of antrum formation when compared with vitrified follicles (47.1% and 20.0% respectively), but without significant difference (p ¼ 0.167). Conclusions The follicles were able to develop, grow and form antrum in the 3D system after vitrification, despite the lower results obtained with the fresh tissue. ResumoObjetivo O presente estudo teve como objetivo avaliar o impacto da vitrificação na viabilidade dos folículos utilizando a cultura in vitro tridimensional (3D). Métodos Foi utilizado tecido ovariano bovino (n ¼ 5) obtido de abatedouros. O córtex foi cortado em pequenos fragmentos de 2 Â 3 Â 0,5 mm, utilizando o tissue slicer e a partir destes fragmentos foram isolados folículos secundários por meio de

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