Fertility results after exocervical insemination using goat semen cryopreserved with extenders based on egg yolk, skim milk, or soybean lecithin

Liang, Jiachong; Allai, Larbi; Lv, Chunrong; Ali, Sikandar; Wu, Guoquan; Quan, Guobo

doi:10.1111/rda.14304

Cited by 7 publications

(2 citation statements)

References 147 publications

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“…Liang et al [96] studied the effects of four extenders for semen cryopreservation (Andromed ® (Andr ® ), Optidyl ® (Opt ® ), P3644 Sigma l-phosphatidylcholine (l-α SL), and skim milk-based (milk) extenders) on the post-thaw quality and fertility of goat semen (Yunshang black bucks, weight 80-100 kg, aged between 2 and 3 years). The intracellular concentrations of ROS in frozen-thawed sperm were determined by staining with 2 , 7dichlorodihydrofluorescein diacetates (H 2 DCFDA) with flow cytometry analysis.…”

Section: Ros Assay In Spermatozoamentioning

confidence: 99%

An Overview on Assay Methods to Quantify ROS and Enzymatic Antioxidants in Erythrocytes and Spermatozoa of Small Domestic Ruminants

Pasciu

Nieddu

Sotgiu

et al. 2023

Animals

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The present review aims to provide an overview of the assay methods for the quantification of ROS and principal enzymatic antioxidants as biomarkers of oxidative stress in erythrocytes and spermatozoa of small domestic ruminants. A complete literature search was carried out in PubMed, Scopus and the World Wide Web using relevant keywords and focusing on the last five years (2018–2023). Among spectrophotometry, fluorometry and chemiluminescence, the most widely used method for ROS assay is fluorometry, probably because it allows to simultaneously assay several ROS, using different probes, with greater economic advantages. Regarding intracellular antioxidant enzymes, recent literature reports only spectrophotometric methods, many of which use commercial kits. The use of a less sensitive but cheapest method is suitable because both erythrocytes and spermatozoa samples are highly concentrated in domestic ruminant species. All methods considered in this review have been found to be appropriate; in general, the differences are related to their costs and sensitivity. Quantification of ROS and enzymatic antioxidant activity in erythrocytes and spermatozoa may find application in the study of the welfare and health status of small domestic ruminants for monitoring livestock production.

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Section: Ros Assay In Spermatozoamentioning

confidence: 99%

An Overview on Assay Methods to Quantify ROS and Enzymatic Antioxidants in Erythrocytes and Spermatozoa of Small Domestic Ruminants

Pasciu

Nieddu

Sotgiu

et al. 2023

Animals

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“…It helps maintain the quality of sperm during the cryopreservation process, including factors like motility, viability, membrane integrity, acrosome integrity and mitochondrial activity. Additionally, soybean lecithin has demonstrated the ability to enhance enzymatic activities in cryopreserved semen (Araújo Silva et al., 2022; Liang et al., 2023; Ratnawati et al., 2023; Salmin et al., 2022; Sharaf et al., 2022). Soybean lecithin nanoparticles have been studied for various applications.…”

Section: Introductionmentioning

confidence: 99%

Low concentrations of soybean lecithin nanoparticles had a positive impact on Holstein bulls' cryopreserved semen

Abdel‐Ghani,

El‐Sharawy,

Zaid

et al. 2024

Reprod Domestic Animals

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Spermatozoa can experience negative changes when subjected to freezing and thawing, including lowered motility, viability and acrosome response. Herein, the effects of different concentrations of soybean lecithin nanoparticles on cryopreserved Holstein bull semen were examined. Semen was collected, cryopreserved and utilized for sperm kinetic parameter analysis following dilution, equilibration and thawing with 0.5% soybean lecithin (E1), the control extender, and 0.75% (E2), 0.5% (E3), 0.25% (E4) and 0.125% (E5) of lecithin nanoparticles. Results revealed that following dilution, the progressive motility (PM) at E3, E4 and E5 of lecithin nanoparticles was higher (p < .05) than it was for E2. After equilibration, compared to the E1, E2, and E3 values, the PM, vitality, normal morphology, membrane integrity and intact acrosome values at the E5 were consistently greater (p < .05). Comparing the percentages of intact acrosome and membrane integrity at E2 and E3 to E4 and E5, a substantial decrease (p < .05) was seen. Following thawing, the percentage of PM improved at E2 and E5, even though their mean PM values were similar (p > .05) compared to E1, E3 and E4. Vigour and progression parameters of sperm (DAP, DCL, DSL, VAP, VCL, VSL and STR) at E5 were higher (p < .05) than those at E1, E2, E3 and E4. In conclusion, the cryopreserved sperm from Holstein bulls revealed outstanding properties both after equilibration and after thawing with 0.125% lecithin nanoparticles, and they were sensitive to high dosages.

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Supplementation of Epimedium polysaccharide (EPS) improves goat semen characteristics following cryopreservation

Li,

Wang,

et al. 2025

Animal Reproduction Science

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Fertility results after exocervical insemination using goat semen cryopreserved with extenders based on egg yolk, skim milk, or soybean lecithin

Cited by 7 publications

References 147 publications

An Overview on Assay Methods to Quantify ROS and Enzymatic Antioxidants in Erythrocytes and Spermatozoa of Small Domestic Ruminants

An Overview on Assay Methods to Quantify ROS and Enzymatic Antioxidants in Erythrocytes and Spermatozoa of Small Domestic Ruminants

Low concentrations of soybean lecithin nanoparticles had a positive impact on Holstein bulls' cryopreserved semen

Supplementation of Epimedium polysaccharide (EPS) improves goat semen characteristics following cryopreservation

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