The most common type of soil in the valleys of large lowland rivers is Fluvisol or Alluvial soil. In order to determine the biogenity of this type of soil along the Western Morava basin, the representation of the total microflora, fungi, actinomycetes, ammonifiers, Azotobacter sp. and oligonitrophiles was examined. The samples were taken from soils used in the most common two different ways (plough fields and meadows). For that were used standard microbiological methods of inoculation a certain decimal dilution on appropriate nutrient media. No correlation was established between the number of examined groups of microorganisms and the way of land use, nor was there a correlation with chemical properties, primarily with pH and organic matter content. A good representation of Azotobacter sp., as an indicator of soil fertility, was found. Agrochemical analyses showed an acidic to neutral reaction, a low to medium percentage of organic matter, a very low to very high content of easily available phosphorus and easily available potassium.