Fertilization and pregnancy using intentionally cryopreserved testicular tissue as the sperm source for intracytoplasmic sperm injection in 10 men with non-obstructive azoospermia

Oates, Robert D.; Mulhall, John P.; Burgess, C.M.; Cunningham, D.L.; Carson, Ronald S.

doi:10.1093/humrep/12.4.734

Cited by 114 publications

(49 citation statements)

References 18 publications

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“…Repeated surgery not only increases the cost but also causes inflammation to the testis, leading to testicular devascularization and fibrosis [Schlegel and Su 1997]. Also, it avoids the need for the couple to both undergo a surgical procedure on the same day [Oates et al 1997].…”

Section: Storage Methods For Small Samplesmentioning

confidence: 99%

Effect of sperm storage and selection techniques on sperm parameters

Sharma

Kattoor

Ghulmiyyah

et al. 2014

Systems Biology in Reproductive Medicine

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Sperm cryopreservation preserves the fertility of cancer patients undergoing chemotherapy, ensures sperm are available at the time of oocyte retrieval in assisted reproductive technology (ART) procedures and avoids the need for repeated sperm extraction surgeries in azoospermic patients. Conventional methods of cryopreservation involve storage in liquid nitrogen (LN 2 ), which causes a significant decline in sperm parameters such as motility and viability and results in DNA damage. Newer methods of sperm cryopreservation such as the LN 2 vapor method, vitrification, and experimental methods such as lyophilization, have significant advantages over the conventional methods in terms of cost effectiveness and ease of use. Density gradient centrifugation (DGC), swim up, and magnetic assisted cell sorting (MACS) can be used prior to or post-cryopreservation to improve post-thaw sperm quality. Cryopreservation in special carriers such as cryoloops and empty zona prevents the loss of small numbers of sperm during cryopreservation. This article will discuss these sperm preservation and selection techniques.

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Section: Storage Methods For Small Samplesmentioning

confidence: 99%

Effect of sperm storage and selection techniques on sperm parameters

Sharma

Kattoor

Ghulmiyyah

et al. 2014

Systems Biology in Reproductive Medicine

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show abstract

“…Thus, testicular tissue cryopreservation is necessary to avoid repeat TESE for subsequent ICSI trials [Nogueira et al 1999], and successful fertilization and pregnancy have been reported using frozen-thawed testicular spermatozoa and testicular tissue [Oates et al 1997;Gianaroli et al 1999]. The fertilization rate using frozen-thawed spermatozoa from men with NOA has been shown to range from 38-70% Gil-Salom et al 1998;Wood et al 2002].…”

Section: Introductionmentioning

confidence: 99%

Effect of testicular spermatozoa on embryo quality and pregnancy in patients with non-obstructive azoospermia

Park

Lee

Lim

et al. 2015

Systems Biology in Reproductive Medicine

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This study was performed to assess and compare the outcomes of testicular sperm extraction (TESE)-intracytoplasmic sperm injection (ICSI) using spermatozoa from fresh and frozen testicular tissue from men with subgroups of non-obstructive azoospermia (NOA). A total of 110 cycles of TESE-ICSI were performed. Patients were classified into one of the following NOA subgroups: hypospermatogenesis (HS), maturation arrest (MA), or Sertoli cell-only syndrome (SCO). Laboratory (fertilization, cleavage stage of embryo, and good quality embryo) and clinical (pregnancy, clinical pregnancy, implantation, and delivery) outcomes were assessed. No statistically significant differences were observed in any of the other measured parameters between the three subgroups of NOA. No significant differences in laboratory outcomes were observed between spermatozoa from fresh and frozen testicular spermatozoa; however, statistically significant differences were observed in the pregnancy and implantation rates between groups (p50.05). The outcomes of using spermatozoa retrieved from fresh testicular tissue in each of the three subgroups were also compared; although clinical outcomes showed low results, no significant differences were observed between the three subgroups. Similarly, no significant differences were observed in spermatozoa retrieved from frozen testicular tissue. Once spermatozoa have been successfully obtained, acceptable laboratory outcomes can be achieved for NOA, whether or not the spermatozoa are cryopreserved. However, satisfactory clinical outcomes may be more difficult to achieve as the results showed in each group of fresh and frozen testicular spermatozoa. Therefore, achieving acceptable clinical pregnancy results and efficient cryopreservation of testicular spermatozoa should be considered in patients with NOA.

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“…Although the use of cryopreserved testicular sperm is not easy because of their low numbers and motility [3,9], there were no differences in fertilization and pregnancy rates for fresh and thawed testicular sperm from men with OA and non-obstructive azoospermia (NOA) [10,11]. Also, fertilization and pregnancies have been reported using thawed testicular sperm and testicular tissue [12,13].…”

Section: Introductionmentioning

confidence: 99%

Efficacy of cryopreservation of embryos generated by intracytoplasmic sperm injection with spermatozoa from frozen testicular tissue

Park

Kim

Lim

et al. 2014

J Assist Reprod Genet

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Purpose To evaluate the viability of frozen embryos generated by intracytoplasmic sperm injection (ICSI) with frozen testicular spermatozoa. Methods A total of 68 fresh embryo transfer (ET) cycles and 85 subsequent frozen-thawed ET (FET) cycles were grouped according to the source of spermatozoa: fresh testicular spermatozoa (TESE) or frozen-thawed testicular spermatozoa (t-TESE). Results There were no significant differences in the age of female patients, number of oocytes, or fertilization rates in fresh ET cycles with TESE (TESE-fresh ET) versus t-TESE (t-TESE-fresh ET). The rate of embryo survival after thawing (95.7 % vs. 94.0 %) was similar in frozen ET cycles (FET) with TESE (TESE-FET) and with t-TESE (t-TESE-FET). While there were significant differences in the proportion of good quality embryos, no statistical differences were found in the pregnancy or clinical abortion rates between the two groups. Moreover, delivery rates were not significantly different. Conclusions Although the proportion of good quality embryos was affected by cryopreservation of testicular tissue, embryo survival rate was not. As well, subsequent pregnancy could be achieved successfully via t-TESE-FET cycles. Therefore, FET is not affected by the cryopreservation of testicular tissue, and avoids further oocyte retrieval and TESE procedures.

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Fertilization and pregnancy using intentionally cryopreserved testicular tissue as the sperm source for intracytoplasmic sperm injection in 10 men with non-obstructive azoospermia

Cited by 114 publications

References 18 publications

Effect of sperm storage and selection techniques on sperm parameters

Effect of sperm storage and selection techniques on sperm parameters

Effect of testicular spermatozoa on embryo quality and pregnancy in patients with non-obstructive azoospermia

Efficacy of cryopreservation of embryos generated by intracytoplasmic sperm injection with spermatozoa from frozen testicular tissue

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