Fertilization in Teleost Fishes: Mechanisms of Sperm-Egg Interactions

Hart, Nathan H.

doi:10.1016/s0074-7696(08)60658-0

Cited by 145 publications

(132 citation statements)

References 248 publications

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“…However, in Rhodeus ocellatus ocellatus (Ohta et al, 1990), the cortical reaction initiated at the vegetative pole towards the animal pole. It should be pointed out that the cortical reaction was not readily detected around the micropyle after fertilization, which agrees with the occurrence of few cortical alveoli in the cytoplasm immediately beneath the micropyle as a strategy to retard the formation of the perivitelline space and allow the entrance of the spermatozoon (Ohta, 1985;Hart, 1990).…”

Section: Discussionsupporting

confidence: 60%

“…The cortical alveoli breakdown and the consequent elevation of the chorion in fertilized eggs are known as the cortical reaction (Hart, 1990). This was observed in B. orbignyanus eggs 10 s after fertilization and lasted up to 5 min.…”

Section: Discussionmentioning

confidence: 99%

“…The spermatozoon penetrates through an opening, the micropyle, located on the egg's animal pole in teleosteans (Kudo, 1980;Kobayashi & Yamamoto, 1981;Hart, 1990;Iwamatsu, 2000). The first spermatozoon that reaches the micropyle and penetrates it will interact with the microvilli found on the oocyte plasmatic membrane (Hart & Donovan, 1983;Iwamatsu, 2000).…”

Section: Introductionmentioning

confidence: 99%

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Structural analysis of fertilization in the fish Brycon orbignyanus

Ganeco

Franceschini-Vicentini

Nakaghi

2009

Zygote

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SummaryIn the present work, we analyzed the structure of oocytes and fertilized eggs of the piracanjuba fish (Brycon orbignyanus) under light and scanning electron microscopy. After inducing spawning, samples were collected at the moment of oocyte extrusion, when oocytes and semen were mixed (time 0), as well as at 10, 20 and 30 s after mixing, every minute up to 10 min, and then at 15 and 20 min. The oocytes are spherical, translucent and greenish with a mean diameter of 1.3 ± 0.11 mm. During the extrusion, cytoplasmic movement was observed in eggs towards the micropyle, characterizing the animal pole. At the moment of fertilization, the cortical cytoplasm showed a higher concentration of cortical alveoli at the animal pole than at the vegetal pole. The cortical alveoli breakdown promoted the elevation of the chorion with a consequent increase in egg diameter (1.95 ± 0.08 mm). The penetration of the spermatozoon promotes the formation of a fertilization cone of spherical external structure, which obstructs the opening of the micropyle. This structure acts as a main mechanism to avoid polyspermy, intercepting the access of supernumerary spermatozoa. Such studies about the reproductive biology of fish are important to species survival and conservation programmes.

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Section: Discussionsupporting

confidence: 60%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Structural analysis of fertilization in the fish Brycon orbignyanus

Ganeco

Franceschini-Vicentini

Nakaghi

2009

Zygote

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“…12). echinoderms (Vacquier, 1981), the cortical layer of the egg was considered to be a gel with specialised viscoelastic mechanical properties (Hart, 1990). Moreover, the egg cortex became increasingly contractile after sperm-egg union, causing a twisting movement of oil droplets toward the animal pole; a meshwork of polymerised actin appeared in the egg cytoplasm and microfilaments became highly organised in the microvilli (Vacquier, 1981;Mabuchi, 1983).…”

Section: The Chorion Structure and Fertilisationmentioning

confidence: 99%

“…Are these instructions and their language influenced by genetic programs of both parents? To answer these questions, the fertilisation process of a fish must be described in the light of reorganisation of the chorion at fertilisation, its physiological roles, the dynamic changes of the egg cortex, the secretory functions of cortical granules, mechanisms of sperm-egg interactions, and mechanical blocking of polyspermy (Lönning and Hagstrom, 1975;Hart, 1990;Yamagami et al, 1992;Griffin et al, 1996;Merrett and Barnes, 1996;Linhart and Kudo, 1997;Chen et al, 1999;Britz and Breining, 2000;Coward et al, 2002).…”

Section: Introductionmentioning

confidence: 99%