Fertilization of bovine oocytes in vitro: Effect of different sources of gametes on fertilization rate and frequency of fertilization anomalies

Pavlok, A.; Torner, H.; Motlı́k, Jan; Fulka, Josef; Kauffold, P; Duschinski, U.

doi:10.1016/0378-4320(88)90014-0

Cited by 48 publications

(20 citation statements)

References 17 publications

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“…The fertilization abnormalities of in vitro matured and fertilized bovine oocytes (Pavlok et al, 1988) are likely to be due to insufficiencies in cytoplasmic maturation and may be partly related to the degree of atresia of the follicle. An irregular distribution of the cortical granula and a defective exocytosis system may result in high rates of polyspermy (Hyttel et al, 1986;Cran and Cheng, 1986).…”

Section: Discussionmentioning

confidence: 99%

Fertilization and developmental competence of bovine oocytes derived from different categories of antral follicles

Pavlok¹,

Lucas‐Hahn²,

Niemann³

1992

Molecular Reproduction Devel

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342

164

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This study investigated the capacity of healthy oocytes derived from follicles of different size to undergo normal fertilization and early embryonic development in vitro and full-term development in vivo. Ovaries were collected from a local abattoir and dissected and classified as follows: group A, greater than 4-8 mm (large); group B, greater than 2-4 mm (medium); and group C, greater than 1-2 mm (small). Oocytes were isolated by puncturing the follicular wall and pressing of the follicle. Only healthy-looking cumulus-oocyte complexes (COC) were used for in vitro maturation. Oocytes were fertilized in vitro by frozen/thawed semen from one bull. Approximately one-fourth of all oocytes was fixed and stained 15-20 h after fertilization, to determine penetration rates. The remaining eggs were transferred to culture medium and were cultivated for up to 9.5 days. Cleavage was observed 65 h and 7 days after fertilization. Expanded, hatching, and hatched blastocysts were fixed and stained after 9.5 days of culture. A total of 86 blastocysts derived from group A and B oocytes was nonsurgically transferred to synchronized recipients 7-8 days after onset of culture. A total of 6.624 follicles were dissected from 265 ovaries, and 1,485 oocytes were isolated from 1,671 group A follicles, 3,509 oocytes from 3,862 group B follicles, and 965 oocytes from 1,091 group C follicles. The fertilization rate, rate of normal fertilization, rate of polyspermy, and rate of other abnormal fertilization features were as follows: group A, 84.9%, 43.2%, 34.1%, 7.6%; group B, 83.6%, 44.8%, 31.1%, 7.8%; and group C, 61.7%, 13.1%, 33.7%, 19.1%.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Discussionmentioning

confidence: 99%

Fertilization and developmental competence of bovine oocytes derived from different categories of antral follicles

Pavlok¹,

Lucas‐Hahn²,

Niemann³

1992

Molecular Reproduction Devel

Self Cite

342

164

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“…The following groups of porcine oocytes were used for assessment of CEEF secretion: fully grown (GVgerminal vesicle) oocytes and oocytes at late diakinesis, metaphase I, and metaphase II prepared by culture of complexes for 0, 22, 27, or 42 h, respectively. The culture medium was M-199 (Sevac, Prague, Czech Republic), buffered with 20 mM NaHCO 3 and 6.25 mM Hepes and supplemented with 5% fetal calf serum (FCS), 0.91 mM sodium pyruvate, 1.62 mM calcium lactate, and antibiotics [20]. This culture medium was supplemented with 0.1 g/ ml FSH (Biogenesis, Poole, England).…”

Section: Isolation and Culture Of Porcine Oocytesmentioning

confidence: 99%

Secretion of Paracrine Factors Enabling Expansion of Cumulus Cells Is Developmentally Regulated in Pig Oocytes1

Nagyová

Vanderhyden

Procházka

2000

Biology of Reproduction

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To demonstrate secretion of cumulus expansion-enabling factor (CEEF) by porcine oocytes, we used an interspecies testing system. Porcine oocytes were used to condition culture medium, and the presence of CEEF was tested using mouse oocytectomized complexes (OOX), which require CEEF for expansion. Follicle-stimulating hormone-stimulated expansion and synthesis of hyaluronic acid (HA) by mouse OOX were assessed after 18 h of culture in media conditioned by porcine oocytes: 1) at different stages of maturation and 2) in which maturation was inhibited with a specific inhibitor of cdk-kinases, butyrolactone I. Fully grown (GV-germinal vesicle), late-diakinesis (LD), metaphase I (MI), and metaphase II (MII) oocytes were prepared by culture of oocyte-cumulus complexes (OCC) for 0, 22, 27, and 42 h, respectively. To block GV breakdown, porcine oocytes were cultured for 27 h in medium supplemented with butyrolactone I (50 microM). Medium conditioned by oocytes in GV, LD, and after butyrolactone I block allowed full expansion of >90% of mouse OOX, whereas oocytes in MI and MII caused disintegration of mouse OOX without cumulus mucification. To measure synthesis of HA by cumulus cells, 25 mouse OOX were cultured in the conditioned media in the presence of 2.5 microCi of D-[6-(3)H]glucosamine hydrochloride. After 18 h, incorporation of the [(3)H]glucosamine into HA was determined either in complexes (retained HA) or in medium plus complexes (total HA). Total HA accumulation by mouse OOX was not different from that of intact OCC. However, oocytes in GV, LD, and after butyrolactone I treatment enabled mouse OOX to retain significantly more HA within the complex than oocytes in MI and MII. The results indicate that secretion of factors that promote the retention of HA within the complex is developmentally regulated during oocyte maturation.

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“…From the onset it must be emphasized that in vitro maturation, by and large, yields oocytes with a lowered potential for developmental capacity in comparison to in vivo matured oocytes (LeibfriedRutledge et al, 1986a(LeibfriedRutledge et al, , 1987Pavlok et al, 1988 (Leibfried and First, 1979;Fukui and Sakuma, 1980;Grimes and Ireland, 1986) or even to be fertilized in vitro (LeibfriedRutledge et al, 1985). Recent (Crozet et al, 1986;Crozet, 1989 This concept has recently been substantiated by Tan and Lu (1990 (Leibfried and First, 1979;Fukui and Sakuma, 1980;Greve et al, 1984;Hensleigh and Hunter, 1985;Xu et al, 1987;Shioya et al, 1988;Greve et al, 1989b;Sirard, 1989;Younis et al, 1989;Kruip et al, 1990;Gordon and Lu, 1990; (Xu et al, 1986a).…”

Section: Cumulus Oocyte Complexesmentioning

confidence: 99%

“…Supplementation of maturation media with additional granulosa cells has been performed in a number of studies and has been found to be absolutely essential for achieving full developmental competence (Staigmiller and Moor, 1984;Critser et al, 1986;Lu et al, 1987;Lutterbach et al, 1987;Fukui and Ono, 1988;Fukui et al, 1988;Leibfried-Rutledge et al, 1989). In our system cumulus oocyte complexes were cultured without supplementary granulosa cells (Madison et al, 1991 (Pavlok et al, 1988 (Lenz et al, 1982(Lenz et al, , 1983b and 2) the GAG, heparin, appears to be superior to chondroitin sulphate (Parrish et al, 1985) in terms of fertilization rates. The proportion of oocytes penetrated by sperm is greatest when fresh semen is incubated with heparin for at least 4 h and frozen-thawed semen for 15 min (Parrish et al, 1986) (1987).…”

Section: Cumulus Oocyte Complexesmentioning

confidence: 99%

In vitro fertilization in cattle: a review

Greve¹,

Madison²

1991

Reprod. Nutr. Dévelop.

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Summary ― In vitro maturation and fertilization of cattle oocytes and subsequent in vitro culture of zygotes and embryos is discussed in the context of recent encouraging data. Mass production of embryos produced in this way in the future will have a great impact on animal production and animal breeding plans, for example the so-called MO-ET (multiple ovulation and embryo transfer) plan which aims at establishing nudeus breeding herds.

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Fertilization of bovine oocytes in vitro: Effect of different sources of gametes on fertilization rate and frequency of fertilization anomalies

Cited by 48 publications

References 17 publications

Fertilization and developmental competence of bovine oocytes derived from different categories of antral follicles

Fertilization and developmental competence of bovine oocytes derived from different categories of antral follicles

Secretion of Paracrine Factors Enabling Expansion of Cumulus Cells Is Developmentally Regulated in Pig Oocytes1

In vitro fertilization in cattle: a review

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