Fertilization of human oocytes by sperm from infertile males after zona pellucida drilling

Gordon, Jon W.; Grunfeld, Larry; Garrisi, G. John; Talansky, Beth E.; Richards, Charlotte J.; Laufer, Neri

doi:10.1016/s0015-0282(16)60010-9

Cited by 166 publications

(27 citation statements)

References 22 publications

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“…During the zonathinning procedure, it was noticed that the thicker, outer layer of the zona appeared to dissolve much more quickly than the thinner, inner layer, which also confirms earlier reports of an apparent bilayered zona when exposed to AT solution (Gordon et al, 1988;Maker and Cohen, 1989a,b). Cohen et al (1988Cohen et al ( , 1989) also reported that some human embryos appear to have uniform zonae whilst others appear to have thin zona patches, which may explain why the time taken for zona thinning with AT varies with individual embryos.…”

Section: Discussionsupporting

confidence: 87%

In-vitro studies on ‘spare’ human preimplantation embryos in culture

Muggleton-Harris¹,

Findlay²

1991

Human Reproduction

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Methods previously used for the biopsy of preimplantation mouse embryos have been applied to individual 'spare' human embryos. Early cleavage-stage human embryos have been cultured and individual blastomeres removed following zonae thinning or drilling. Embryos have also been cultured to the blastocyst stage for the biopsy of three to five trophectoderm cells. Both the biopsied embryo and the biopsied cells have been allowed to develop and/or grow in vitro.

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Section: Discussionsupporting

confidence: 87%

In-vitro studies on ‘spare’ human preimplantation embryos in culture

Muggleton-Harris¹,

Findlay²

1991

Human Reproduction

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“…Also, numbers of acrosome-reacted spermatozoa were only slightly increased below this concentration. Despite the uncertainties about the mechanisms involved, this finding may be relevant to some new techniques that are being developed to assist fertilization in vitro for treatment of male infertility, including microinjection of human spermatozoa into the perivitelline space, zona drilling and zona opening (Gordon et al, 1988;Mann, 1988;Maker & Cohen, 1989;Odawara & Lopata, 1989). Because these techniques allow spermatozoa access to the oolemma without their binding to or penetration of the ZP it will probably be necessary to stimulate the AR to increase the chances of spermatozoa fusing with the oolemma.…”

Section: Discussionmentioning

confidence: 94%

Inducing the human acrosome reaction with a calcium ionophore A23187 decreases sperm-zona pellucida binding with oocytes that failed to fertilize in vitro

Liu¹,

Baker²

1990

Reproduction

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The human acrosome reaction was induced with the calcium ionophore A23187 and the proportion of reacted spermatozoa was determined with fluorescein-labelled Pisum sativum agglutinin. Human oocytes that failed to fertilize in vitro were used to test binding of spermatozoa to the zona pellucida (ZP) and oolemma. Differential labelling of spermatozoa with fluorescein and rhodamine was used to control for variability in the oocytes. Spermatozoa labelled with one fluorochrome were treated with A23187 and mixed with equal numbers of motile untreated control spermatozoa labelled with the other fluorochrome. The mixture was incubated with zona-intact and zona-free oocytes for 4 h. The sperm-ZP binding ratio of test to control spermatozoa was significantly decreased with increasing proportions of acrosome reacted spermatozoa. In contrast, the sperm-oolemma binding ratio was significantly increased with A23187 treatment. This suggests that acrosome-reacted spermatozoa do not bind to the human ZP.

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“…Localized chemical treatment of oocytes with acidified Tyrode's solution prior to sperm exposure, also known as zona drilling, was another method of introducing deliberate gaps in the ZP to facilitate sperm entry (Gordon & Talansky 1986). However, its application was limited by the 50% rate of polyspermy in drilled oocytes (Gordon et al 1988). In addition, zona drilling involved exposure to a solution of pH 2.3, thereby increasing the risk of chemical damage to oocytes (Gordon et al 1988).…”

Section: Assisted Fertilizationmentioning

confidence: 99%

“…However, its application was limited by the 50% rate of polyspermy in drilled oocytes (Gordon et al 1988). In addition, zona drilling involved exposure to a solution of pH 2.3, thereby increasing the risk of chemical damage to oocytes (Gordon et al 1988). Zona drilling was refined further by creating a partial opening in the ZP with mechanical force.…”

Section: Assisted Fertilizationmentioning

confidence: 99%

The pioneering of intracytoplasmic sperm injection: historical perspectives

Rosenwaks

Pereira

2017

Reproduction

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Intracytoplasmic sperm injection (ICSI) has often been heralded as a ground-breaking technique that has transformed the treatment of couples with infertility. By injecting a single spermatozoon into the cytoplasm of the oocyte, ICSI bypasses the zona pellucida and increases the chances of fertilization and subsequent embryo development, independent of semen parameters. Ever since the first live births using ICSI were reported in 1992, ICSI has become the mainstay of treating male factor infertility as well as overcoming fertilization failure associated with conventional in vitro insemination. Today, ICSI is utilized in nearly 66% of all assisted reproductive treatments worldwide and has resulted in the birth of millions of babies. The primary goal of this review is to provide historical perspectives about the pioneering of ICSI. We begin by highlighting the scientific work of early investigators who elucidated the mechanisms central to mammalian fertilization. Furthermore, we briefly discuss how these findings contributed to the development of IVF for the treatment of infertility. We then emphasize the shortcomings of IVF in treating severe forms of male factor infertility and enumerate the micromanipulation techniques that were developed to circumvent these shortcomings. Finally, we indicate how the inadequacies of these micromanipulation techniques lead to the inception, application and popularity of ICSI.Reproduction (2017) 154 F71-F77

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Fertilization of human oocytes by sperm from infertile males after zona pellucida drilling

Cited by 166 publications

References 22 publications

In-vitro studies on ‘spare’ human preimplantation embryos in culture

In-vitro studies on ‘spare’ human preimplantation embryos in culture

Inducing the human acrosome reaction with a calcium ionophore A23187 decreases sperm-zona pellucida binding with oocytes that failed to fertilize in vitro

The pioneering of intracytoplasmic sperm injection: historical perspectives

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