Fertilizing ability and survivability of rooster sperm diluted with a novel semen extender supplemented with serine for practical use on smallholder farms

Chankitisakul, Vibuntita; Boonkum, Wuttigrai; Kaewkanha, Theerapat; Pimprasert, Maruay; Ratchamak, Ruthaiporn; Authaida, Supakorn; Thananurak, Pachara

doi:10.1016/j.psj.2022.102188

Cited by 15 publications

(11 citation statements)

References 30 publications

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“…The number of damaged spermatozoa plasma membranes and dead spermatozoa makes abnormal spermatozoa increase. These results follow the study on squid conducted by Chankitisakul et al (2022) that the dilution of gummy semen with physiological NaCl and storage time at 27 o C affect the abnormality of spermatozoa, with the higher degree of dilution and the duration of storage, the higher the abnormality of the spermatozoa.…”

Section: Abnormality Of Muscovy Duck Semensupporting

confidence: 88%

Diluent and Storage Time Effect on Sperm Abnormality and MDA Level in Muscovy Duck Semen at 27oC

Andaruisworo,

Tanjungsari,

Yuniati

et al. 2023

J. Med. Vet.

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This study aimed to determine sperm abnormality and MDA level in Muscovy duck semen in different dilution and storage at 27oC. This study was used in the level of dilution of glutinous semen with a long time store differently at 27oC. In this study, the parameters of the mass motility of spermatozoa were used individual motility, spermatozoa abnormalities, and activity test. A Muscovy duck, healthy and have a high libido were used then divided into treatment groups i.e. (A0) 0; (A1) 5 times; (A2) 10 times; and (A3) 15 times, the second factor as a subplot was the storage time (BO) 0; (B1) 60 minutes; (B2) 120 minutes; and (B3) 180 minutes stored at 27oC with 3 replications. In results, this study was reported abnormalities at a dilution rate in A1 group of 5 times with a shelf life in B1 group of 60 minutes and in B2 group of 120 minutes.

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Section: Abnormality Of Muscovy Duck Semensupporting

confidence: 88%

Diluent and Storage Time Effect on Sperm Abnormality and MDA Level in Muscovy Duck Semen at 27oC

Andaruisworo,

Tanjungsari,

Yuniati

et al. 2023

J. Med. Vet.

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“…For instance, arginine can participate in the ornithine cycle and convert the ammonia produced in the body into non-toxic urea through the ornithine cycle, thereby decreasing the blood ammonia concentration ( 48 ). Serine has been demonstrate to possess several metabolic functions including providing precursors for the synthesis of phosphoglycerides, neurotransmitters, sphingolipids, proteins and phosphatidylserine ( 49 , 50 ). Tryptophan can participate in the renewal of plasma proteins in animals, promote the role of riboflavin and the synthesis of niacin and heme, which contributes to increasing the antibodies in the fetuses of pregnant animals and promote lactation in dairy cows and sows ( 51 ).…”

Section: Discussionmentioning

confidence: 99%

Complete genome analysis of Bacillus subtilis derived from yaks and its probiotic characteristics

Wang

Lin

et al. 2023

Front. Vet. Sci.

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Probiotics have attracted attention due to their multiple health benefits to the host. Yaks inhabiting the Tibetan plateau exhibit excellent disease resistance and tolerance, which may be associated with their inner probiotics. Currently, research on probiotics mainly focuses on their positive effects on the host, but information regarding their genome remains unclear. To reveal the potential functional genes of Bacillus subtilis isolated from yaks, we sequenced its whole genome. Results indicated that the genomic length of Bacillus subtilis was 866,044,638 bp, with 4,429 coding genes. The genome of this bacteria was composed of one chromosome and one plasmid with lengths of 4,214,774 and 54,527 bp, respectively. Moreover, Bacillus subtilis contained 86 tRNAs, 27 rRNAs (9 16S_rRNA, 9 23S_rRNA, and 9 5S_rRNA), and 114 other ncRNA. KEGG annotation indicated that most genes in Bacillus subtilis were associated with biosynthesis of amino acids, carbon metabolism, purine metabolism, pyrimidine metabolism, and ABC transporters. GO annotation demonstrated that most genes in Bacillus subtilis were related to nucleic acid binding transcription factor activity, transporter activity, antioxidant activity, and biological adhesion. EggNOG uncovered that most genes in Bacillus subtilis were related to energy production and conversion, amino acid transport and metabolism, carbohydrate transport and metabolism. CAZy annotation found glycoside hydrolases (33.65%), glycosyl transferases (22.11%), polysaccharide lyases (3.84%), carbohydrate esterases (14.42%), auxiliary activities (3.36%), and carbohydrate-binding modules (22.59%). In conclusion, this study investigated the genome and genetic properties of Bacillus subtilis derived from yaks, which contributed to understanding the potential prebiotic mechanism of probiotics from the genetic perspective.

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“…Semen was collected using the dorsal abdominal massage technique [ 15 ]. The individual ejaculate was collected in a 1.5 mL microtube containing 0.1 mL of IGGKph diluent, as described by Chankitisakul et al [ 23 ]. The sperm samples were transferred to the laboratory within 20 min of semen collection.…”

Section: Methodsmentioning

confidence: 99%

“…The artificial insemination method was performed as described by Chankitisakul et al [ 23 ]. Briefly, fertilizing capacity was tested using intravaginal insemination of the hens at days 0 (once-weekly artificial insemination), days 0 and 3 (twice-weekly artificial insemination), and days 0, 3, and 6 (thrice-weekly artificial insemination) with a dose of 0.2 mL of semen (150 × 10 6 spz/dose and 250 × 10 6 spz/dose).…”

Section: Methodsmentioning

confidence: 99%

Fertility and Insemination Characteristics of Sperm Storage Tubules in Old Thai-Native Hens

Kheawkanha,

Chankitisakul,

Pimprasert

et al. 2024

Animals

Self Cite

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We aimed to evaluate the effects of sperm concentration (150–250 × 106 spz/dose) and insemination frequency (once, twice, and thrice weekly) on fertility and sperm storage tubule (SST) characteristics. The SSTs were classified into five categories: namely, SSTs having an unscorable (SST1), empty (SST2), low (SST3), medium (SST4), and high (SST5) sperm count after insemination. The results showed that only insemination frequency affected the fertility rate (p < 0.05). The highest fertility was found in the thrice-weekly insemination group; however, this rate was not significantly different from that for the twice-weekly insemination group, except on day 7, while the once-weekly insemination group showed the lowest fertility rate (p < 0.05) from day four onward. On day 1, the SST characteristics showed no differences among the various insemination frequencies. On day 4, the SST2 and SST3 categories increased in the once-weekly insemination group (p < 0.05), while the SST4 and SST5 categories decreased compared to the twice- and thrice-weekly insemination groups (p < 0.05). On day 7, only the thrice-weekly insemination group maintained a level of SST5 category tubules like that measured on day 1 (p > 0.05). In summary, the insemination dose of 150 × 106 sperm was enough for fertilization, and thrice-weekly insemination was the appropriate frequency in old Thai native hens for maintaining a high sperm density in the SSTs throughout the week.

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Fertilizing ability and survivability of rooster sperm diluted with a novel semen extender supplemented with serine for practical use on smallholder farms

Cited by 15 publications

References 30 publications

Diluent and Storage Time Effect on Sperm Abnormality and MDA Level in Muscovy Duck Semen at 27oC

Diluent and Storage Time Effect on Sperm Abnormality and MDA Level in Muscovy Duck Semen at 27oC

Complete genome analysis of Bacillus subtilis derived from yaks and its probiotic characteristics

Fertility and Insemination Characteristics of Sperm Storage Tubules in Old Thai-Native Hens

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