Fertilizing Capacity of Frozen Human Spermatozoa

Bunge, R.G.; Sherman, J.K.

doi:10.1038/172767b0

Cited by 250 publications

(93 citation statements)

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“…In human, it takes 74 days to generate functional sperm from spermatogonia (Clermont 1972). Techniques to routinely freeze and thaw functional human sperm have been in existence for over 50 years (Bunge and Sherman 1953). The success with human sperm was presaged by studies in livestock.…”

Section: Gamete Diversitymentioning

confidence: 99%

Mammalian diversity: gametes, embryos and reproduction

Behringer

Eakin

Renfree

2006

Reprod. Fertil. Dev.

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Abstract. The class Mammalia is composed of approximately 4800 extant species. These mammalian species are divided into three subclasses that include the monotremes, marsupials and eutherians. Monotremes are remarkable because these mammals are born from eggs laid outside of the mother's body. Marsupial mammals have relatively short gestation periods and give birth to highly altricial young that continue a significant amount of 'fetal' development after birth, supported by a highly sophisticated lactation. Less than 10% of mammalian species are monotremes or marsupials, so the great majority of mammals are grouped into the subclass Eutheria, including mouse and human. Mammals exhibit great variety in morphology, physiology and reproduction. In the present article, we highlight some of this remarkable diversity relative to the mouse, one of the most widely used mammalian model organisms, and human. This diversity creates challenges and opportunities for gamete and embryo collection, culture and transfer technologies.

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Section: Gamete Diversitymentioning

confidence: 99%

Mammalian diversity: gametes, embryos and reproduction

Behringer

Eakin

Renfree

2006

Reprod. Fertil. Dev.

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“…Cryopreservation of human sperm is now routinely practiced in Assisted Reproductive Technology and Andrology laboratories for a range of reasons includ- 1 Concept Fertility Centre, King Edward Memorial Hospital, Subiaco, Western Australia, Australia. 2 To whom correspondence should be addressed at Concept Fertility Centre, King Edward Memorial Hospital, 374 Bagot Rd., Subiaco, Western Australia 6008, Australia; e-mail: pete@ conceptfert.…”

Section: Introductionmentioning

confidence: 99%

Vitality of Oligozoospermic Semen Samples Is Improved by Both Swim-Up and Density Gradient Centrifugation Before Cryopreservation

Counsel

Bellinge

Burton

2004

J Assist Reprod Genet

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Purpose : To ascertain whether washing sperm from oligozoospermic and normozoospermic samples before cryopreservation improves post-thaw vitality. Methods : Normozoospermic (n = 18) and oligozoospermic (n = 16) samples were divided into three aliquots. The first aliquot remained untreated and the second and third aliquots were subjected to the swim-up and discontinuous density gradient sperm washing techniques respectively. Vitality staining was performed, samples mixed with cryopreservation media and frozen. Spermatozoa were thawed, stained, and vitality quantified and expressed as the percentage of live spermatozoa present. Results : Post-thaw vitality in untreated aliquots from normozoospermic samples (24.9% ± 2.3; mean ± SEM) was significantly higher (unpaired t-tests; P < 0.01) than untreated oligozoospermic samples (11.9% ± 2.3). Post-thaw vitality was significantly higher after swim-up in normozoospermic samples (35.6% ± 2.1; P < 0.001; one-way ANOVA) and oligozoospermic samples (27.7% ± 1.7; P < 0.01). Density gradient centrifugation significantly improved postthaw vitality in oligozoospermic (22.4% ± 1.0; P < 0.01) but not normozoospermic (30.8% ± 1.8) samples. Conclusions : Sperm vitality in cryopreserved oligozoospermic samples was improved by both the swim-up and density gradient centrifugation washing techniques prior to freezing.

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“…The fertility cryopreservation has been an ever increasing field since Polge et al, (1949), in United Kingdom, produced the first chicks from cryopreserved fowl sperm, in which, partly by error, a sample was frozen with additional glycerol. Afterwards, Bunge & Sherman (1953) found that human spermatozoa treated with 10% glycerol and freezed with 'dry ice' survived in high precentage. Edwards et al (1969) fertilized the first human egg in vitro.…”

Section: Historymentioning

confidence: 99%

Fertility Cryopreservation

Chian¹,

Quinn²

2010

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Protecting the reproductive potential of young patients undergoing cancer therapy is increasingly important. With modern treatment protocols, 80% of patients can be expected to survive. It has been estimated that up to one in 250 young adults will be a survivor of childhood cancer in the future; infertility, however, may be a consequence. As a wide range of fertility preservation methods are increasingly offered by clinicians, this systematic and comprehensive textbook dealing with the cryobiology, technology and clinical approach to this therapy will be essential reading to infertility specialists, embryologists, oncologists, cryobiologists, ObGyns, andrologists, and urologists with an interest in fertility preservation. Fertility Cryopreservation reviews all the techniques of this increasingly important field within reproductive medicine. It covers the basic principles of pertinent cryobiology, and contains major sections on the different therapies available, written by international specialists combining experience from both academic centers and commercial industries.

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Fertilizing Capacity of Frozen Human Spermatozoa

Cited by 250 publications

References 4 publications

Mammalian diversity: gametes, embryos and reproduction

Mammalian diversity: gametes, embryos and reproduction

Vitality of Oligozoospermic Semen Samples Is Improved by Both Swim-Up and Density Gradient Centrifugation Before Cryopreservation

Fertility Cryopreservation

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