Ferulic acid: a key component in grass lignocellulose recalcitrance to hydrolysis

Oliveira, Dyoni Matias de; Finger-Teixeira, Aline; Mota, Thatiane Rodrigues; Salvador, Victor Hugo; Moreira-Vilar, Flávia Carolina; Molinari, Hugo Bruno Correa; Mitchell, R. A. C.; Marchiosi, Rogério; Ferrarese-Filho, Osvaldo; Santos, Wanderley Dantas dos

doi:10.1111/pbi.12292

Cited by 225 publications

(177 citation statements)

References 113 publications

(174 reference statements)

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“…FA and p -CA are important in cell wall polymer modification and can be ester linked to xylan [30, 40]. We used polysaccharide analysis by carbohydrate gel electrophoresis (PACE) to determine the xylan structure of the OsAT10 overexpression switchgrass [41].…”

Section: Resultsmentioning

confidence: 99%

Overexpression of a rice BAHD acyltransferase gene in switchgrass (Panicum virgatum L.) enhances saccharification

Jones

Eudes

et al. 2018

BMC Biotechnol

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BackgroundSwitchgrass (Panicum virgatum L.) is a promising bioenergy feedstock because it can be grown on marginal land and produces abundant biomass. Recalcitrance of the lignocellulosic components of the switchgrass cell wall to enzymatic degradation into simple sugars impedes efficient biofuel production. We previously demonstrated that overexpression of OsAT10, a BAHD acyltransferase gene, enhances saccharification efficiency in rice.ResultsHere we show that overexpression of the rice OsAT10 gene in switchgrass decreased the levels of cell wall-bound ferulic acid (FA) in green leaf tissues and to a lesser extent in senesced tissues, and significantly increased levels of cell wall-bound p-coumaric acid (p-CA) in green leaves but decreased its level in senesced tissues of the T0 plants under greenhouse conditions. The engineered switchgrass lines exhibit an approximate 40% increase in saccharification efficiency in green tissues and a 30% increase in senesced tissues.ConclusionOur study demonstrates that overexpression of OsAT10, a rice BAHD acyltransferase gene, enhances saccharification of lignocellulosic biomass in switchgrass.Electronic supplementary materialThe online version of this article (10.1186/s12896-018-0464-8) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Overexpression of a rice BAHD acyltransferase gene in switchgrass (Panicum virgatum L.) enhances saccharification

Jones

Eudes

et al. 2018

BMC Biotechnol

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“…The exact ferulic acid biosynthesis pathway as well as the form in which it is transferred onto arabinoxylans are both still a matter of debate. The current hypothesis is that the formation of feruloylated compounds involves FA conjugates, which are very likely CoA-esters [90,98,99]. FeruloylCoA would result from methylation of a caffeoyl-CoA precursor by a CCoAOMT (Fig.…”

Section: Arabinoxylan Feruloylation and Ferulate Cross-linkagesmentioning

confidence: 97%

Breeding maize for silage and biofuel production, an illustration of a step forward with the genome sequence

et al. 2016

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“…This could be due to the fact that the residual AX is not tightly associated with D-glucans (mixedlinkage β-D-glucan or cellulose), or due to the reduced enzymatic accessibility as a consequence of the presence of FA dehydrodimer crosslinks. 50 In a further attempt, a feruloyl esterase (FE, from family 1A) and an endo-1,4-β-D-xylanase (X, from family GH10) were used alone and in synergy to disrupt the phenolic crosslinked structures of the residue. The FE alone does not show significant activity towards the residue, but its activity increased after 16 h of incubation when combined with the xylanase (Fig.…”

Section: Valorization Of the Residue By Sequential Enzymatic Treatmenmentioning

confidence: 99%

“…55 The radical scavenging activity of selected samples is presented in Fig. 6, whereas the results on all the samples together with the half maximal effective concentration (EC 50 ) values are presented in the ESI Table S7. † The alkaline extract did not show statistically significant scavenging activity compared with the controls.…”

Section: Antioxidant Activities Of the Ax Fractionsmentioning

confidence: 99%

Sequential fractionation of feruloylated hemicelluloses and oligosaccharides from wheat bran using subcritical water and xylanolytic enzymes

et al. 2017

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Wheat bran is a major by-product of cereal production that still has limited use for advanced nutritional and material applications. A sequential process using subcritical water, membrane filtration and selective enzymatic treatments has been designed for the combined fractionation of functional high molar mass hemicelluloses (over 10 5 g mol −1 ) and oligosaccharides from wheat bran. This process not only offers increased total solid yield compared with conventional protocols based on alkaline extraction, but it also preserves the inherent functionalities of the phenolic groups that substitute the carbohydrate structures of the extracted hemicelluloses. Feruloylated arabinoxylans (F-AX) with high molar mass and significant radical scavenging activity can be isolated from the subcritical water extract. Structurally different oligosaccharides, including mixed-linkage β-D-glucan oligosaccharides (BGOs) and arabinoxylo-oligosaccharides (AXOs) can be recovered from the eluent after membrane filtration. The crosslinked residue after subcritical water extraction was further treated with xylanolytic enzymes to release valuable feruloylated arabinoxylo-oligosaccharides (FAXOs). The oligo-and polysaccharide fractions isolated from this sequential process show great potential for use as prebiotic or platform chemicals, and as polymeric matrices for carbohydrate-based materials with radical scavenging properties, respectively. IntroductionCereal processing generates large volumes of by-products that have not been exploited to their full potential for advanced nutritional and material applications. 1 Each ton processed by the wheat milling industry generates up to 0.25 tons of wheat bran that corresponds to 14-16% of the cereal kernel. 2 Due to its content of valuable biomacromolecules, such as polysaccharides (mainly hemicelluloses) and phenolic compounds, wheat bran has gained renewed interest as a raw material to be used in second generation biorefineries. 2-4Xylan is one of the most abundant biopolymers in nature as a major non-cellulosic component of plant cell walls. In particular, arabinoxylan (AX) constitutes the main hemicellulose in grasses and cereal grains, with an average composition of 20-40% depending on the species and tissue considered. View Article OnlineView Journal | View Issue substitutions. 9 Other methods have also been developed for the extraction and purification of AX from cereal by-products, including acid pretreatment, 11 microwave assisted extraction, 12 ultrasound assisted extraction, 13,14 and steam explosion extraction. 10,15 As an alternative, subcritical water extraction (SWE), also called pressurized hot water extraction (PHWE), appears as a promising green method for the isolation of hemicellulose fractions with preserved molecular functionalities and high molecular weight. The use of subcritical conditions (at temperature below critical point and pressure high enough to maintain liquid state) changes dramatically the properties of water, including density surface tension, polarity, vis...

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Ferulic acid: a key component in grass lignocellulose recalcitrance to hydrolysis

Cited by 225 publications

References 113 publications

Overexpression of a rice BAHD acyltransferase gene in switchgrass (Panicum virgatum L.) enhances saccharification

Overexpression of a rice BAHD acyltransferase gene in switchgrass (Panicum virgatum L.) enhances saccharification

Breeding maize for silage and biofuel production, an illustration of a step forward with the genome sequence

Sequential fractionation of feruloylated hemicelluloses and oligosaccharides from wheat bran using subcritical water and xylanolytic enzymes

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