Fervidobacterium thailandense sp. nov., an extremely thermophilic bacterium isolated from a hot spring

Kanoksilapatham, Wirojne; Pasomsup, Patlada; Keawram, Porranee; Cuecas, Alba; Guisado, María del Carmen Portillo; Gonzalez, Juan M.

doi:10.1099/ijsem.0.001463

Cited by 26 publications

(6 citation statements)

References 17 publications

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“…The 64 C TP samples were rich in genera belonging exclusively to the thermophilic families Thermaceae (obligately oxidative, phyla Deinococcus-Thermus) and Dictyoglomaceae (phyla Dictyoglomi) (Albuquerque & Da Costa, 2014;Bergquist & Morgan, 2014), along with taxa from hyperthermophilic and thermophilic phyla Aquificae and Thermotogae. In consistent with previous studies, Tatta Pani hot spring also has relative high abundance of OTUs belonging to the genus Fervidobacterium from phylum Thermotogae (Kanoksilapatham et al, 2016;Wang et al, 2010). Furthermore, the TP64 sample was exclusively rich in the thermophilic methanotroph Methylothermus, a specialized group of bacteria that uses methane and methanol as sole carbon and energy sources (Tsubota et al, 2005).…”

Section: Discussionsupporting

confidence: 88%

16S amplicon sequencing of microbial communities in enriched and non-enriched sediments of non-volcanic hot spring with temperature gradients

Yasir

Qureshi

Azhar

2021

PeerJ

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Microorganisms in geothermal springs can offer insights into the fundamental and applied study of extremophiles. However, low microbial abundance and culturing requirements limit the ability to analyze microbial diversity in these ecosystems. In this study, culture-dependent and culture-independent techniques were used to analyze sediment samples from the non-volcanic Tatta Pani hot springs in district Poonch of Azad Kashmir. Microbial composition, temperature gradient, and enrichment effects on rare taxa were evaluated. In total, 31 distinct bacterial phyla and 725 genera were identified from the non-enriched Tatta Pani hot spring sediment samples, and 33 distinct bacterial phyla and 890 genera from the enriched sediment samples. Unique phyla specimens from the enriched samples included Candidatus Cloacimonetes, Caldiserica, and Korarchaeota archaea. The enriched samples yielded specific microbiota including 805 bacteria and 42 archaea operational taxonomic units with 97% similarity, though decreased thermophilic microbiota were observed in the enriched samples. Microbial diversity increased as temperature decreased. Candidate novel species were isolated from the culture-dependent screening, along with several genera that were not found in the 16S amplicon sequencing data. Overall, the enriched sediments showed high microbial diversity but with adverse changes in the composition of relatively dominant bacteria. Metagenomic analyses are needed to study the diversity, phylogeny, and functional investigation of hot spring microbiota.

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Section: Discussionsupporting

confidence: 88%

16S amplicon sequencing of microbial communities in enriched and non-enriched sediments of non-volcanic hot spring with temperature gradients

Yasir

Qureshi

Azhar

2021

PeerJ

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“…pennivorans DSM9070 (NC_017095) [ 17 ], F . islandicum AW-1 (NZ_CP014334) [ 18 , 19 ] and a new isolate [ 20 ], Fervidobacterium thailandense strain FC2004 (LWAF01000000) were used in this analysis. Annotated transposases in these four genomes and homology searching (using blast algorithms) [ 21 ] resulted in the detection of a number of transposases.…”

Section: Methodsmentioning

confidence: 99%

Evidence of horizontal gene transfer by transposase gene analyses in Fervidobacterium species

2017

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Horizontal Gene Transfer (HGT) plays an important role in the physiology and evolution of microorganisms above all thermophilic prokaryotes. Some members of the Phylum Thermotogae (i.e., Thermotoga spp.) have been reported to present genomes constituted by a mosaic of genes from a variety of origins. This study presents a novel approach to search on the potential plasticity of Fervidobacterium genomes using putative transposase-encoding genes as the target of analysis. Transposases are key proteins involved in genomic DNA rearrangements. A comprehensive comparative analysis, including phylogeny, non-metric multidimensional scaling analysis of tetranucleotide frequencies, repetitive flanking sequences and divergence estimates, was performed on the transposase genes detected in four Fervidobacterium genomes: F. nodosum, F. pennivorans, F. islandicum and a new isolate (Fervidobacterium sp. FC2004). Transposase sequences were classified in different groups by their degree of similarity. The different methods used in this study pointed that over half of the transposase genes represented putative HGT events with closest relative sequences within the phylum Firmicutes, being Caldicellulosiruptor the genus showing highest gene sequence proximity. These results confirmed a direct evolutionary relationship through HGT between specific Fervidobacterium species and thermophilic Firmicutes leading to potential gene sequence and functionality sharing to thrive under similar environmental conditions. Transposase-encoding genes represent suitable targets to approach the plasticity and potential mosaicism of bacterial genomes.

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“…Descriptions of thermophilic and extremophilic keratinolytic representatives from genera Fervidobacterium sp. ( 54 ), Thermoanaerobacter sp. ( 51 ) and Bacillus sp.…”

Section: Microbial Sources Of Keratinasesmentioning

confidence: 99%

Microbial keratinases: enzymes with promising biotechnological 4 applications

Vidmar

Vodovnik

2018

Food Technol. Biotechnol.

110

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SUMMARYKeratin is a complex and structurally stable protein found in human and animal hard tissues, such as feathers, wool, hair, hoof and nails. Some of these, like feathers and wool, represent one of the main sources of protein-rich waste with significant potential to be transformed into value-added products such as feed, fertilizers or bioenergy. A major limitation impeding valorization of keratinous substrates is their recalcitrant structure and resistance to hydrolysis by common proteases. However, specialized keratinolytic enzymes produced by some microorganisms can efficiently degrade these substrates. Keratinases have already found a purpose in pharmaceutical, textile and leather industries. However, their wider implementation in other processes, such as cost-effective (pre)treatment of poultry waste, still requires optimization of production and performance of the available enzymes. Here we present a comprehensive review covering molecular properties and characteristics of keratinases, their classification, traditional and novel approaches in discovery of novel enzymes, production, characterization, improvement and biotechnological applications.

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Fervidobacterium thailandense sp. nov., an extremely thermophilic bacterium isolated from a hot spring

Cited by 26 publications

References 17 publications

16S amplicon sequencing of microbial communities in enriched and non-enriched sediments of non-volcanic hot spring with temperature gradients

16S amplicon sequencing of microbial communities in enriched and non-enriched sediments of non-volcanic hot spring with temperature gradients

Evidence of horizontal gene transfer by transposase gene analyses in Fervidobacterium species

Microbial keratinases: enzymes with promising biotechnological 4 applications

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