Feselol Enhances the Cytotoxicity and DNA Damage Induced by Cisplatin in 5637 Cells

Abstract

Transitional cell carcinoma (TCC), which is the most common type of bladder cancer, shows resistance to chemotherapeutic agents due to the overexpression of drug effl ux pumps. In this study, the effects of feselol, a sesquiterpene coumarin extracted from Ferula badrakema, on cisplatin cytotoxicity were investigated in 5637 cells, a TCC subline. Cell viability and DNA lesion were evaluated by thiazolyl blue tetrazolium bromide and comet assays, respectively. Feselol had no signifi cant cytotoxic effect in 5637 cells but at 32 μg/mL it increased the cytotoxicity of 1 μg/mL cisplatin by 37% after 24 h. Furthermore, the comet assay revealed that DNA damage induced by cisplatin in 5637 cells is enhanced by 31% when used in combination with feselol. Therefore, feselol might be considered as an effective reversal agent for future in vivo and clinical studies.

Sesquiterpene Coumarin Ethers with Selective Cytotoxic Activities from the Roots of Ferula huber-morathii Peşmen (Apiaceae) and Unequivocal Determination of the Absolute Stereochemistry of Samarcandin

“…In a study conducted by Soltani et al [ 67 ], the cytotoxic activities of feselol ( 3 ) on the HCT-116, HeLa, A549, and A2780 cancer cell lines were found to be 28, 35, 26, and 20 µM, respectively. Furthermore, some studies reported that feselol ( 3 ) did not show any cytotoxic activity on the bladder (TCC, 5637), K562, HeLa, and stomach (AGS) cancer cell lines [ 14 , 71 , 72 , 73 , 74 ].…”