Fetal Cells in Maternal Blood: A Comparison of Methods for Cell Isolation and Identification

Christensen, B.; Philip, John; Kølvraa, Steen; Lykke-Hansen, Lene; Hromadníková, Ilona; Gohel, D.; Lörch, Thomas; Plesch, Andreas; Bang, J.; Smidt‐Jensen, Steen; Hertz, J. B.; Djursing, Henning

doi:10.1159/000082432

Cited by 17 publications

(14 citation statements)

References 29 publications

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“…For NRBCs, immunomagnetic separation relies on the depletion of CD45+ cells, or the positive enrichment of CD71+ cells. 186 NRBCs have diameters ranging from 9 to 12 μm and are reported to be more deformable than white blood cells. 29 …”

Section: Applications Of Rare Cell Isolation and Analysismentioning

confidence: 99%

Rare cell isolation and analysis in microfluidics

et al. 2014

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Rare cells are low-abundance cells in a much larger population of background cells. Conventional benchtop techniques have limited capabilities to isolate and analyze rare cells because of their generally low selectivity and significant sample loss. Recent rapid advances in microfluidics have been providing robust solutions to the challenges in the isolation and analysis of rare cells. In addition to the apparent performance enhancements resulting in higher efficiencies and sensitivity levels, microfluidics provides other advanced features such as simpler handling of small sample volumes and multiplexing capabilities for high-throughput processing. All of these advantages make microfluidics an excellent platform to deal with the transport, isolation, and analysis of rare cells. Various cellular biomarkers, including physical properties, dielectric properties, as well as immunoaffinities, have been explored for isolating rare cells. In this Focus article, we discuss the design considerations of representative microfluidic devices for rare cell isolation and analysis. Examples from recently published works are discussed to highlight the advantages and limitations of the different techniques. Various applications of these techniques are then introduced. Finally, a perspective on the development trends and promising research directions in this field are proposed.

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Section: Applications Of Rare Cell Isolation and Analysismentioning

confidence: 99%

Rare cell isolation and analysis in microfluidics

et al. 2014

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“…For more than a decade, we have investigated the presence of fetal cells in maternal blood with the ultimate aim of cell‐based NIPT. Initially, our research strategy was to define the types of fetal cells that circulate in the maternal blood, and based on this, choose specific markers that can lead to the isolation and identification of pure fetal cells for subsequent analyses.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide copy number analysis on DNA from fetal cells isolated from the blood of pregnant women

Kølvraa¹,

Singh²,

Normand

et al. 2016

Prenat Diagn

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100

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“…In the past, a lot of effort was put into analysis of intact fetal cells in the maternal circulation [Bianchi et al, 1992;Gänshirt-Ahlert et al, 1993;Bianchi, 1999;Lim et al, 2001;Christensen et al, 2005], with limited success. Although these cells are a pure source of fetal genomic material and a widely available technique like FISH could be used for analysis, isolation of these scarce fetal cells until now has proven problematic and laborious [Hahn et al, 2011].…”

Section: Application Of Array-cgh In the Light Of Future Developmentsmentioning

confidence: 99%

From Karyotyping to Array-CGH in Prenatal Diagnosis

Lichtenbelt

Knoers²,

Schuring-Blom³

2011

Cytogenet Genome Res

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Conventional karyotyping detects chromosomal anomalies in up to 35% of pregnancies with fetal ultrasound anomalies, depending on the number and type of these anomalies. Extensive experience gained in the past decades has shown that prenatal karyotyping is a robust technique which can detect the majority of germline chromosomal anomalies. For most of these anomalies the phenotype is known. In postnatal diagnosis of patients with congenital anomalies and intellectual disability, array-CGH/SNP array has become the first-tier investigation. The higher abnormality detection yield and its amenability to automation renders array-CGH also suitable for prenatal diagnosis. As both findings of unclear significance and unexpected findings may be detected, studies on the outcome of array-CGH in prenatal diagnosis were initially performed retrospectively. Recently, prospective application of array-CGH in pregnancies with ultrasound anomalies, and to a lesser extent in pregnancies referred for other reasons, was studied. Array-CGH showed an increased diagnostic yield compared to karyotyping, varying from 1–5%, depending on the reason for referral. Knowledge of the spectrum of array-CGH anomalies detected in the prenatal setting will increase rapidly in the years to come, thus facilitating pre- and posttest counseling. Meanwhile, new techniques like non-invasive prenatal diagnosis are emerging and will claim their place. In this review, we summarize the outcome of studies on prenatal array-CGH, the clinical relevance of differences in detection rate and range as compared to standard karyotyping, and reflect on the future integration of new molecular techniques in the workflow of prenatal diagnosis.

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Fetal Cells in Maternal Blood: A Comparison of Methods for Cell Isolation and Identification

Cited by 17 publications

References 29 publications

Rare cell isolation and analysis in microfluidics

Rare cell isolation and analysis in microfluidics

Genome-wide copy number analysis on DNA from fetal cells isolated from the blood of pregnant women

From Karyotyping to Array-CGH in Prenatal Diagnosis

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