Fetal liver kinase 1 is a receptor for vascular endothelial growth factor and is selectively expressed in vascular endothelium.

Quinn, Tim; Peters, Kevin G.; Vries, Carlie de; Ferrara, Napoleone; Williams, Lewis T.

doi:10.1073/pnas.90.16.7533

Cited by 669 publications

(380 citation statements)

References 26 publications

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“…We therefore refer to the murine flt-1 receptor as VEGFRlLIflt-1. The apparent Kd for VEGF binding to murine VEGFRlAlt-1, as calculated by using a nonlinear regression curve, was 90 pM and, as determined by Scatchard analysis, 114 pM, being somewhat higher than recently described for human VEGFRlAlt-1 (Quinn et al, 19931, but considerably lower than the reported K, for VEGF binding to murine VEGFRZLI flk-1 (approximately 600 pM; Millauer et al, 1993;Quinn et al, 1993).…”

Section: Isolation and Characterization Of A Mouse Flt-1 Cdnamentioning

confidence: 54%

Coordinate expression of vascular endothelial growth factor receptor‐1 (fit‐1) and its ligand suggests a paracrine regulation of murine vascular development

Breier

Clauss

Risau

1995

Developmental Dynamics

281

152

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Vascular endothelial growth factor (VEGF) is a candidate regulator of blood vessel growth during embryonic development and in tumors. To evaluate the role of VEGF receptor-l/ flt-1 (VEGFRl/flt-1) in the development of the vascular system, we have characterized the murine homolog of the human flt-1 gene and have analyzed its expression pattern during mouse embryogenesis. Receptor binding studies using transfected COS cells revealed that the murine flt-1 gene encodes a high affinity receptor for VEGF. The apparent K, for VEGF binding, as determined by Scatchard analysis, was 114 pM, demonstrating that VEGFRl/flt-1 has a higher affinity to VEGF than VEGF receptor-2/flk-l (VEGFR2/flk-l). By in situ hybridization, VEGFRl/flt-1 was detected in the yolk sac mesoderm already at the early stages of vascular development, while the receptor ligand was expressed in the entire endoderm of 7.5-day mouse embryos. A comparison with VEGFR2/flk-1 showed that the two receptors shared a common expression domain in the yolk sac mesoderm, but were expressed at different sites in the ectoplacental cone. The differential expression of the two VEGF receptors persisted in the developing placenta, where VEGFRl/flt-1 mRNA was detected in the spongiotrophoblast layer, whereas VEGFR2/ flk-1 transcripts were present in the labyrinthine layer which is the site of VEGF expression. In the embryo proper, VEGFRlIflt-1 mRNA was specifically localized in blood vessels and capillaries of the developing organs, closely resembling the pattern of VEGFR2/flk-1 transcript distribution. In the developing brain, the expression of VEGF receptors in the perineural capillary plexus and in capillary sprouts which have invaded the neuroectoderm correlated with endothelial cell proliferation and brain angiogenesis. The data are consistent with the hypothesis that VEGF and its receptors have an important function both in the differentiation of the endothelial lineage and in the neovascularization of developing organs, and act in a paracrine fashion. o 1995 Wiley-Liss, Inc.

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Section: Isolation and Characterization Of A Mouse Flt-1 Cdnamentioning

confidence: 54%

Coordinate expression of vascular endothelial growth factor receptor‐1 (fit‐1) and its ligand suggests a paracrine regulation of murine vascular development

Breier

Clauss

Risau

1995

Developmental Dynamics

281

152

View full text Add to dashboard Cite

show abstract

“…8 Another high-affinity VEGFR, Flt-1, binds VEGF about 10 times stronger than KDR, however Flt-1 activation does not significantly stimulate angiogenesis. 9,10 Both receptors, Flt-1 and KDR, have similar structure with extracellular regions consisting of seven domains, 1-7. 10,11 There exists another naturally occurring soluble form of Flt-1 (sFlt-1) that contains only the extracellular domains and has the same VEGF-binding affinity as full-length Flt-1.…”

Section: Introductionmentioning

confidence: 99%

“…9,10 Both receptors, Flt-1 and KDR, have similar structure with extracellular regions consisting of seven domains, 1-7. 10,11 There exists another naturally occurring soluble form of Flt-1 (sFlt-1) that contains only the extracellular domains and has the same VEGF-binding affinity as full-length Flt-1. 11,12 The VEGF-binding function of Flt-1 has been mapped to the second domain.…”

Section: Introductionmentioning

confidence: 99%

Novel anti-VEGF chimeric molecules delivered by AAV vectors for inhibition of retinal neovascularization

Pecháň¹,

Rubin²,

Lukason³

et al. 2008

Gene Ther

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“…VEGF binds to two receptor-type tyrosine kinases, Flt-1 (VEGF receptor-1) (Shibuya et al, 1990;de Vries et al, 1992) and KDR/ Flk-1 (VEGF receptor-2) (Quinn et al, 1993), and to membrane protein neuropilin-1, which does not contain a tyrosine kinase domain (Soker et al, 1998). Flt-1 and KDR/Flk-1 have seven immunoglobulin-like repeats in the extracellular domain, a single transmembrane region, and a tyrosine kinase domain in the intracellular domain that is interrupted by a kinaseinsert domain.…”

Section: Introductionmentioning

confidence: 99%

Roles of two VEGF receptors, Flt-1 and KDR, in the signal transduction of VEGF effects in human vascular endothelial cells

Kanno¹,

Oda²,

Abé³

et al. 2000

Oncogene

265

175

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Fetal liver kinase 1 is a receptor for vascular endothelial growth factor and is selectively expressed in vascular endothelium.

Abstract: ABSTRACT

Cited by 669 publications

References 26 publications

Coordinate expression of vascular endothelial growth factor receptor‐1 (fit‐1) and its ligand suggests a paracrine regulation of murine vascular development

Coordinate expression of vascular endothelial growth factor receptor‐1 (fit‐1) and its ligand suggests a paracrine regulation of murine vascular development

Novel anti-VEGF chimeric molecules delivered by AAV vectors for inhibition of retinal neovascularization

Roles of two VEGF receptors, Flt-1 and KDR, in the signal transduction of VEGF effects in human vascular endothelial cells

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