Fetal Rat Adrenal Gland Steroidogenesis in vitro in Prolonged Pregnancy

R, Klepac

doi:10.1159/000241422

Cited by 3 publications

(2 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Controls were injected with 0.2 ml of 0.9%o NaCl and the carrier solution used. Other group of animals received a second injection of progesterone on day 22, and gestation was prolonged by 1 day (Klepac, 1981). Conception was assumed by the presence of spermatozoa in the vagina, and gestational age was verified by the foetal weight.…”

Section: Experimental Animal Treatmentmentioning

confidence: 99%

Regulation of rat foetal lipogenesis in brown adipose tissue in vivo and in isolated brown adipocytes during the last day of, and after prolonged, gestation

Roncero¹,

Lorenzo²,

Benito³

1987

Biochemical Journal

View full text Add to dashboard Cite

Rates of lipogenesis in foetal isolated brown adipocytes from 22-day-pregnant rats were significantly increased by lactate plus pyruvate as major substrates in the incubation medium, in comparison with the endogenous rates. Insulin stimulated foetal brown-adipocyte lipogenesis, and adrenaline or noradrenaline and isoprenaline decreased lipogenesis. Glucagon had no effect on the lipogenic rate in brown adipocytes. Progesterone administration to the mother significantly increased the rates of lipogenesis in brown adipose tissue and in isolated brown adipocytes from 22-day foetuses. Prolongation of gestation by progesterone to day 23 decreased the rates of brown-adipose-tissue lipogenesis in vivo and in isolated cells in the post-mature foetuses.

show abstract

Section: Experimental Animal Treatmentmentioning

confidence: 99%

Regulation of rat foetal lipogenesis in brown adipose tissue in vivo and in isolated brown adipocytes during the last day of, and after prolonged, gestation

Roncero¹,

Lorenzo²,

Benito³

1987

Biochemical Journal

View full text Add to dashboard Cite

show abstract

“…However maternal adrenal and fetal adrenal gland may also contribute to the pregnenolone production since both these tissues have cytochrome p450 side chain cleavage enzyme that converts cholesterol to pregnenolone [6,7]. Some authors argue that fetal liver may even contribute to the pregnenolone production by providing the cholesterol as a substrate to the fetal adrenal gland [3].…”

Section: Discussionmentioning

confidence: 99%

Using the oocyte donation model to identify early trophoblast pregnenolone production

Licciardi

Tan

2013

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose To investigate production of progesterone's precursor, pregnenolone, in the early oocyte donation pregnancy. Methods Pregnenolone and progesterone were measured on luteal days 21, 28, 35, 60 and 80. Progesterone was measured via the Immulite system, pregnenolone by liquid chromatography separation with tandem mass spectrometric detection. Results Progesterone rose significantly from days 35 today 60. Pregnenolone likewise rose significantly from days 35-60, but at a much higher rate, with an increase of 57 % by day 60, 75 % to day 80. The increase in pregnenolone was statistically more significant than the increase in progesterone (p<.05). Conclusions This is the first report describing that progesterone's precursor, pregnenolone, increases with time in the very early pregnancy. Because no corpus luteum is present in oocyte recipients, the main source of pregnenolone is the early placenta. Measurements of pregnenolone may provide information concerning early trophoblast function and may represent a method of assessing placental competency.

show abstract

Distribution and metabolism of maternal progesterone in the uterus, placenta, and fetus during rat pregnancy

Benbow

Waddell²

1995

Biology of Reproduction

View full text Add to dashboard Cite

This study examined the in vivo distribution and metabolism of maternal progesterone (P4) in the rat uterus at Day 16 of pregnancy, i.e., the time of maximal P4 secretion, and at Day 22, one day prior to parturition. Arterial and uterine venous blood samples were collected at 20-min intervals from rats (n = 5 per group) infused with [3H]-P4 for 2 h. Placentas, fetuses, and uterine tissue (myometrium and decidua) were obtained just prior to the end of the infusion; and total tritium, [3H]-P4, and lipid-soluble and water-soluble metabolite concentrations were determined in all blood and tissue samples. Irreversible extraction of P4 by the uterus and its contents was 54.2 +/- 6.0% (mean +/- SEM) on Day 16, and this was at least maintained to Day 22 (64.7 +/- 7.4%). Because uterine blood flow increases dramatically over this period, the maintenance of high uterine P4 extraction is likely to have contributed partly to the 41% rise in the metabolic clearance rate of P4 between Day 16 (147 +/- 16 ml/min per kg) and Day 22 (207 +/- 13 ml/min per kg). Uterine tissue levels of [3H]-P4 exceeded (1.6-fold) those in arterial blood on Day 16, but this difference was not evident at Day 22. In contrast, the concentration of [3H]-P4 in the placenta was lower than that in arterial blood at Day 16 (66% lower) and Day 22 (25% lower), even though total tritium concentrations were similar at these sites. [3H]-P4 was also lower in fetal tissue compared with maternal arterial blood on both days of pregnancy.(ABSTRACT TRUNCATED AT 250 WORDS)

show abstract

Fetal Rat Adrenal Gland Steroidogenesis in vitro in Prolonged Pregnancy

Cited by 3 publications

References 12 publications

Regulation of rat foetal lipogenesis in brown adipose tissue in vivo and in isolated brown adipocytes during the last day of, and after prolonged, gestation

Regulation of rat foetal lipogenesis in brown adipose tissue in vivo and in isolated brown adipocytes during the last day of, and after prolonged, gestation

Using the oocyte donation model to identify early trophoblast pregnenolone production

Distribution and metabolism of maternal progesterone in the uterus, placenta, and fetus during rat pregnancy

Contact Info

Product

Resources

About