Fetal Tissue Collection from Spontaneous Abortions: A Report from a Single Centre

Abstract

Due to immunological immaturity, the fetus is the ideal recipient as well as donor of haemopoietic stem cells (HSCs); thus intrauterine therapy may prove to be effective in all major haemopoietic disorders when early prenatal diagnosis is available. In man, ‘fetus-to-fetus’ transplantation has demonstrated the possibility of grafting donor HSCs and reconstituting immunodeficient fetuses. The limitations of fetal tissue use for transplantation derive from the origin of tissues from elective abortions. Early and late live spontaneous abortions may constitute an alternative to elective abortions, but are widely considered as unsuitable for fetal tissue collection because of rapid loss of viability and/or infections. The aim of this retrospective study was to assess the number of live abortions in a population of women who underwent spontaneous abortion in a single centre. In a 19-month period, 9 spontaneous abortions alive at the moment of delivery and 8 with a heart beat at the last ultrasound scan before abortion were recorded. In 1 case, fetal liver (FL) harvesting was easily performed and the tissue was cryopreserved, subsequently thawed and injected into a monkey fetus. This case shows the feasibility of this approach. The majority of cases reviewed consisted of late abortions. These findings clearly show that fetal tissue collection from spontaneous abortions is feasible for research purposes, for postnatal FL transplantation and for intrauterine transplantation, provided that depletion of more mature cells is performed when FL of later gestational age are used. The number of fetuses collected could be further augmented provided that a multicentre network for fetal tissue collection is established.

Comparative Study of Hemopoietic Precursors from Fetal and Adult Bone Marrow: Utilization of Stem Cells Derived from Miscarriages

“…More over, our results strongly support the concept of the age-related and tissue-specific ontogen ic differences in the phenotypic composition and functional capacity of FBM and ABM [ 12,[33][34][35][36], These results strongly support the utilization of spontaneously aborted fetal tis sue, provided that the tissue is well selected in terms of viability and freedom of any contam ination (viral, bacterial, fungal, and genetic). The high number of CD34+ cells, when com pared to other sources of stem cells, clearly suggested that this is the stem cell population of choice for hemopoietic stem cell reconstitu tion and gene therapy as an ideal target cell population [37], In fact, data obtained by oth er investigators confirmed the efficacy of stem cells from miscarriages [38,39], Thus, it sup ports our initial hypothesis that this untapped source of viable cells could be utilized. The 380 Feial Diagn Tlicr 1996:11:373-382 M ichcjda/Bcllanti/M azum der/Vcrm a/ Wu tissue should be examined for any contamina tion and stored properly in a tissue bank for utilization in life-saving transplantations [13,14].…”