Fetal vasculogenesis and angiogenesis in human placental villi

Demi, R.; Kaufmann, Peter; Castellucci, Mario; Erbengi, T.; Kotowski, A.

doi:10.1016/0143-4004(89)90079-9

Cited by 47 publications

(72 citation statements)

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“…The process of angiogenesis is characterized by sprouting of new capillaries from existing vessels and is an essential component of placental development [1]. The distribution of mast cells (MC) may be related to the local requirement of some MC-derived mediators, e. g. histamine (HA), or vascular endothelial growth factor (VEGF).…”

Section: Introductionmentioning

confidence: 99%

Mast cell-derived vascular endothelial growth factor (VEGF) and microvascular density in diabetic placentae

Szukiewicz

Maślińska

et al. 2003

Inflammation Research

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Section: Introductionmentioning

confidence: 99%

Mast cell-derived vascular endothelial growth factor (VEGF) and microvascular density in diabetic placentae

Szukiewicz

Maślińska

et al. 2003

Inflammation Research

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“…Placental vasculogenesis, which starts in early tertiary villi, is completed at the beginning of the last trimester by the appearance of a basal lamina (Demir et al 1989). Little angiogenesis is observed later in gestation.…”

Section: Discussionmentioning

confidence: 98%

“…Bars a 100 µm; b, c 10 µm& / f i g . c : mary phenotype has been described for these cells in early vasculogenesis (Dempsey 1972;Demir et al 1989). The cell heterogeneity observed in culture depends on the strength of the enzyme digestion rather than the cell line differentiation from stem cells.…”

Section: Discussionmentioning

confidence: 98%

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Phenotype of cultured fetal perivascular cells from human placenta studied by scanning electron microscopy

Challier

Galtier

et al. 1996

Anatomy and Embryology

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The phenotype of perivascular placental cells has previously been studied using tissue sections from the fetal villi. The examination of these cells in culture by scanning electron microscopy gives us the opportunity to observe their three-dimensional phenotypes and associations outside their normal constraints. Human umbilical endothelial cells, which have a phenotype comparable to that observed in other studies, seem more flattened in culture than in their usual environment. Microvascular endothelial cells did not attain an epithelioid phenotype with close contacts between cells but formed a network of branched, elongated cells with phagocytotic activity. Some circular associations were observed when using a gelatinized matrix. Microvascular pericytes were large, flattened cells with an irregular border that pushed up nodular associations on a gelatin matrix. Chorioplacental myocytes adopted a network template comparable to that developed by microvascular endothelial cells. However, these elongated cells were thicker, without microvilli, and superficial filaments could be observed. In culture, confluent endothelial cells from the umbilical cord or microvascular pericytes associated as nodules reached a cell phenotype close to their in vivo counter-parts. This attainment of an in vivo phenotype remains questionable for chorioplacental myocytes. Microvascular endothelial cells, however, though there was sparse formation of circular associations, remained far from their in vivo phenotype.

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“…); 5 samples at 43-49 days (seventh week p.c.)] in the Wrst trimester of pregnancy were obtained after legal termination of pregnancy by curettage for medical or psychosocial reasons, which were unlikely to aVect placental structure and function (Demir et al 1989). None of the normal pregnancies were receiving hormone treatment.…”

Section: Tissue Collectionmentioning

confidence: 99%

Lung surfactant proteins in the early human placenta

Satı

Seval-Celik

Demir

2009

Histochem Cell Biol

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Pulmonary surfactant is a complex mixture of phospholipids and four surfactant-associated proteins (SP-A, SP-B, SP-C and SP-D). The biological functions of SP-A and SP-D are primarily twofold, namely surfactant homeostasis and host defense. The hydrophobic surfactant proteins, SP-B and SP-C, are required for achieving the optimal surface tension reducing properties of surfactant by promoting the rapid adsorption of surfactant phospholipids along the alveolar surface. Despite the promising findings, only little is known about the extrapulmonary distribution of these proteins. Therefore, in this study, the presence of SP-A, SP-B, SP-C and SP-D in early human placenta has been investigated. First-trimester placental tissues (22-56 days) were obtained from women undergoing curettage during normal pregnancies. In parallel tissue sections, vimentin, cytokeratin-7 and CD-68 immunostainings were used for the identification of mesenchymal cells, trophoblast cells and Hofbauer cells, respectively. According to immunohistochemistry (IHC) results, SP-A, SP-B, SP-C and SP-D immunoreactivities with different staining intensities were observed in trophoblastic layers of chorionic villous tree, trophoblastic cell columns, stromal cells, Hofbauer cells, angiogenic cell cords and vascular endothelium. Fetal hematopoietic cells showed a variable staining pattern for all four surfactant proteins ranging from none to strong intensity. Western blotting of tissue extracts confirmed our IHC results. The presence of surfactant glycoproteins in early human placenta may yield a very important feature of surfactants during first trimester and enables further studies of the role of surfactants in various pregnancy complications.

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Fetal vasculogenesis and angiogenesis in human placental villi

Cited by 47 publications

References 0 publications

Mast cell-derived vascular endothelial growth factor (VEGF) and microvascular density in diabetic placentae

Mast cell-derived vascular endothelial growth factor (VEGF) and microvascular density in diabetic placentae

Phenotype of cultured fetal perivascular cells from human placenta studied by scanning electron microscopy

Lung surfactant proteins in the early human placenta

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