Fetuin-B, a Liver-Derived Plasma Protein Is Essential for Fertilization

Dietzel, Eileen; Wessling, Jennifer; Floehr, Julia; Schäfer, Cora; Ensslen, Silke; Denecke, Bernd; Rösing, Benjamin; Neulen, Joseph; Veitinger, Thomas; Spehr, Marc; Tropartz, Tanja; Tolba, René; Renné, Thomas; Egert, Angela; Schorle, Hubert; Gottenbusch, Yuliya; Hildebrand, André; Yiallouros, Irene; Stöcker, Walter; Weiskirchen, Ralf; Jahnen-Dechent, Willi

doi:10.1016/j.devcel.2013.03.001

Cited by 106 publications

(115 citation statements)

References 39 publications

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“…Fetuin-B, also identified in our Sia-glycoprobe fractions, is another protein that regulates gamete fusion by restraining ovastacin protease activity, thereby maintaining ZP permeability until the first spermatozoon intrudes. After penetration, ovastacin overrides fetuin-B and initiates ZP hardening, thus preventing polyspermy (59).…”

Section: Discussionmentioning

confidence: 99%

Identification of Bovine Sperm Surface Proteins Involved in Carbohydrate-mediated Fertilization Interactions

Defaus

Sánchez

Andreu

et al. 2016

Molecular & Cellular Proteomics

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Glycan-protein interactions play a key role in mammalian fertilization, but data on the composition and identities of protein complexes involved in fertilization events are scarce, with the added complication that the glycans in such interactions tend to differ among species. In this study we have used a bovine model to detect, characterize and identify sperm lectins relevant in fertilization. Given the complexity of the sperm-toward-egg journey, two important aspects of the process, both primarily mediated by protein-sugar interactions, have been addressed: (1) formation of the sperm reservoir in the oviductal epithelium, and (2) gamete recognition (oocytesperm interaction). Using whole sperm cells and a novel affinity capture method, several groups of proteins with different glycan specificities, including 58 hitherto unreported as lectins, have been identified in sperm surface, underscoring both the efficacy of our selective approach and the complex composition and function of sperm. Based on these results and previous data, we suggest that sperm surface proteins play significant roles in fertilization events such as membrane remodeling, transport, protection and function, thus supporting the hypothesis that rather than a simple lock-and-key model, mammalian fertilization relies on a complex interactome involving multiple ligands/receptors and recognition/binding events. Molecular & Cellular

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Section: Discussionmentioning

confidence: 99%

Identification of Bovine Sperm Surface Proteins Involved in Carbohydrate-mediated Fertilization Interactions

Defaus

Sánchez

Andreu

et al. 2016

Molecular & Cellular Proteomics

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“…In addition, it has been suggested that liver-originated circulating glycoprotein fetuin-B is essentially required to inhibit premature cleavage of ZP2 by inhibiting ovastacin (Dietzel et al, 2013).…”

Section: Box2 Potential Limitations Of Knockout Approachesmentioning

confidence: 99%

The cell biology of mammalian fertilization

2013

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Fertilization is the process by which eggs and spermatozoa interact, achieve mutual recognition, and fuse to create a zygote, which then develops to form a new individual, thus allowing for the continuity of a species. Despite numerous studies on mammalian fertilization, the molecular mechanisms underpinning the fertilization event remain largely unknown. However, as I summarize here, recent work using both gene-manipulated animals and in vitro studies has begun to elucidate essential sperm and egg molecules and to establish predictive models of successful fertilization.

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“…Descriptions of the protease web are difficult to assemble, as many proteases remain poorly studied and characterized. Likewise, many proteases have no described inhibitors and many predicted inhibitors have unknown protease targets and deorphanization examples are uncommon [41].…”

Section: Introductionmentioning

confidence: 99%