FGF‐2‐loaded collagen scaffolds attract cells and blood vessels in rat oral mucosa

Jansen, Richard G.; Kuppevelt, Toin H. Van; Daamen, Willeke F.; Kuijpers-Jagtman, Anne Marie; Hoff, Johannes W. Von den

doi:10.1111/j.1600-0714.2009.00763.x

Cited by 19 publications

(13 citation statements)

References 39 publications

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“…In the bone‐forming test, histological samples obtained 35 d after implantation of the FGF‐2‐loaded scaffold frequently showed an increase in giant cells around the scaffold, suggesting active wound healing and remodeling. A previous study also revealed giant cells in FGF‐2‐loaded collagen scaffold after implantation in rat oral mucosa . It has been demonstrated that β‐TCP degradation promotes an osteogenic marker of osteoblastic cells, alkaline phosphatase activity, resulting from calcium ion release .…”

Section: Discussionmentioning

confidence: 82%

“…Regenerative scaffolds should induce rapid reconstructive tissue ingrowth through wound healing. Up‐regulation of the ingrowth of regenerative tissue and blood vessels into the scaffold is generally relevant to the morphology of the scaffold . When clinically applied as a bone‐graft material, β‐TCP controls pore characteristics such as pore volume fraction, size and structure.…”

mentioning

confidence: 99%

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Bone augmentation using a highly porous PLGA/β‐TCP scaffold containing fibroblast growth factor‐2

Yoshida

Miyaji

Otani

et al. 2014

J of Periodontal Research

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Section: Discussionmentioning

confidence: 82%

mentioning

confidence: 99%

Bone augmentation using a highly porous PLGA/β‐TCP scaffold containing fibroblast growth factor‐2

Yoshida

Miyaji

Otani

et al. 2014

J of Periodontal Research

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“…Jansen et al implanted FGF‐2 loaded collagen scaffolds in the palate of rats, which led to an increased number of host cells and a higher vascularization rate. Furthermore, there was a reduced number of myofibroblasts, decreasing the wound contraction, one of the major problems of palate wound healing.…”

Section: Fgf‐2/fgfr‐1 Expression In Normal Oral Mucosa and During Oramentioning

confidence: 99%

Expression of FGF‐2/FGFR‐1 in normal mucosa, salivary gland, preneoplastic, and neoplastic lesions of the oral cavity

Mariz

Soares

Morais

et al. 2018

J Oral Pathology Medicine

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Fibroblast growth factor 2 (FGF-2) is a multifunctional cytokine expressed in several tissues and involved in a wide variety of biologic activities, with one low molecular weight (LMW) protein present in the cytosol, which is secreted, acting via its receptors (FGFRs), and four high molecular weight (HMW) proteins located in the nucleus. Fibroblast growth factor receptor (FGFR) family has four (FGFR1-4) transmembrane tyrosine kinase receptors expressed on several cell types, and FGFR-1 has been indicated as a potential molecular target in several types of cancer, including oral squamous cell carcinoma (OSCC). The FGF-2/FGFR-1 expression has been studied in the oral cavity, and it was associated with the wound repair process, the development of benign and malignant salivary gland tumors, besides being related to oral potentially malignant disorders (OPMDs) and OSCC. Hence, we critically review the currently available data on FGF-2/FGFR-1 expression in the normal mucosa and lesions of the oral cavity.

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“…Basic fibroblast growth factor (bFGF), a member of fibroblast growth factor family, has various biological functions both in bone regeneration and angiogenesis. [19,[22][23][24] Expressed in osteoblasts and stored in extracellular matrix, [25] bFGF plays a vital role in mitogenesis, cellular migration, and differentiation. Several studies suggested that bFGF is essential for normal development and formation of bone, tendons, and ligaments in the skeleton.…”

Section: Introductionmentioning

confidence: 99%

Preparation and preliminaryin vitroevaluation of a bFGF-releasing heparin-conjugated poly(ε-caprolactone) membrane for guided bone regeneration

Cao

Song

Yao

et al. 2015

Journal of Biomaterials Science, Polymer Edition

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In an effort to improve guided bone regeneration (GBR), we successfully fabricated a novel basic fibroblast growth factor (bFGF)-releasing heparin-conjugated poly(ε-caprolactone) membrane (hep-PCL/bFGF). This material has a porous microstructure with smooth and rough pore walls before and after heparinization, respectively. Our FTIR analyses indicated that chemical bonds were formed between PCL and heparin with a new amide C=O band at 1660 cm(-1) and a band at 3400 cm(-1) that can be attributed to -OH stretching in cross-linked heparin. We showed that bFGF was released from hep-PCL/bFGF in a continuous pattern, which remained for 3 weeks. We evaluated MG63 cell proliferation and biocompatibility of GBR membrane by a CCK-8 assay and a live/dead assay. The CCK-8 results revealed that the hep-PCL/bFGF group had superiority compared to other groups. Furthermore, cell morphology of hep-PCL membrane exhibited larger projected areas than those of PCL surfaces based on scanning electron microscopy analysis and immunofluorescent staining of cell cytoskeleton and vinculin expression. Our alkaline phosphatase activity assay also confirmed better performance of the hep-PCL/bFGF group. These results suggested that this novel hep-PCL/bFGF membrane is suitable for osteoblast-like cells to attach, proliferate, and differentiate. Therefore, the hep-PCL/bFGF membrane has potential to be a biodegradable membrane for GBR and warrants further investigation.

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FGF‐2‐loaded collagen scaffolds attract cells and blood vessels in rat oral mucosa

Abstract: FGF-2-loaded scaffolds displayed a faster influx of host cells, an increased rate of vascularization, and a reduced differentiation of myofibroblasts. These scaffolds might therefore be highly suitable for intra-oral reconstructions, such as cleft palate repair.

Cited by 19 publications

References 39 publications

Bone augmentation using a highly porous PLGA/β‐TCP scaffold containing fibroblast growth factor‐2

Bone augmentation using a highly porous PLGA/β‐TCP scaffold containing fibroblast growth factor‐2

Expression of FGF‐2/FGFR‐1 in normal mucosa, salivary gland, preneoplastic, and neoplastic lesions of the oral cavity

Preparation and preliminaryin vitroevaluation of a bFGF-releasing heparin-conjugated poly(ε-caprolactone) membrane for guided bone regeneration

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