FGF primes angioblast formation by inducing ETV2 and LMO2 via FGFR1/BRAF/MEK/ERK

Chen, Peng Chieh; Hsueh, Ya Wen; Lee, Yi‐Hsuan; Tsai, Hung Wen; Tsai, Kuen Jer; Chiang, Po Min

doi:10.1007/s00018-020-03630-8

Cited by 3 publications

(1 citation statement)

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“…The design of the short‐hairpin RNAs (shRNAs) targeting LMP1 (full sequences for cloning in Table ) was based on splashRNA and cloned into the donor vector below by Gibson assembly 23 . The forward and reverse oligos were cloned into an AAVS1 locus‐donor vector expressing GFP and the shRNA in tandem as described, 24 except that the Thy1.1 was replaced by GFP for staining‐free assessment of the induction. Five micrograms of the donor vector and 5 µg of the px459‐based AAVS1‐targeting vector were electroporated into the recipient cells with NEPA21 system.…”

Section: Methodsmentioning

confidence: 99%

Epstein‐Barr virus latent membrane protein‐1 upregulates autophagy and promotes viability in Hodgkin lymphoma: Implications for targeted therapy

et al. 2021

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Hodgkin lymphoma (HL) is composed of neoplastic Hodgkin and Reed-Sternberg cells in an inflammatory background. The neoplastic cells are derived from germinal center B cells that, in most cases, are infected by Epstein-Barr virus (EBV), which may play a role in tumorigenesis. Given that EBV-latent membrane protein 1 (LMP1) regulates autophagy in B cells, we explored the role of autophagy mediated by EBV or LMP1 in HL. We found that EBV-LMP1 transfection in HL cells induced a modest increase in autophagy signals, attenuated starvation-induced autophagic stress, and alleviated autophagy inhibition-or doxorubicin-induced cell death. LMP1 knockdown leads to decreased autophagy LC3 signals. A xenograft mouse model further showed that EBV infection significantly increased expression of the autophagy marker LC3 in HL cells. Clinically, LC3 was expressed in 15% (19/127) of HL samples, but was absent in all cases of nodular lymphocyte-predominant and lymphocyte-rich classic HL cases. Although expression of LC3 was not correlated with EBV status or clinical outcome, autophagic blockade effectively eradicated LMP1-positive HL xenografts with better efficacy than LMP1-negative HL xenografts. Collectively, these results suggest that EBV-LMP1 enhances autophagy and promotes the viability of HL cells.This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Section: Methodsmentioning

confidence: 99%

Epstein‐Barr virus latent membrane protein‐1 upregulates autophagy and promotes viability in Hodgkin lymphoma: Implications for targeted therapy

et al. 2021

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Overexpression of interleukin-20 correlates with favourable prognosis in diffuse large B-cell lymphoma

Lu¹,

Chiang²,

Huang³

et al. 2023

Pathology

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The regulatory role of pioneer factors during cardiovascular lineage specification – A mini review

Sierra-Pagan

Garry

2022

Front. Cardiovasc. Med.

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Cardiovascular disease (CVD) remains the number one cause of death worldwide. Ischemic heart disease contributes to heart failure and has considerable morbidity and mortality. Therefore, alternative therapeutic strategies are urgently needed. One class of epigenetic regulators known as pioneer factors has emerged as an important tool for the development of regenerative therapies for the treatment of CVD. Pioneer factors bind closed chromatin and remodel it to drive lineage specification. Here, we review pioneer factors within the cardiovascular lineage, particularly during development and reprogramming and highlight the implications this field of research has for the future development of cardiac specific regenerative therapies.

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FGF primes angioblast formation by inducing ETV2 and LMO2 via FGFR1/BRAF/MEK/ERK

Cited by 3 publications

References 45 publications

Epstein‐Barr virus latent membrane protein‐1 upregulates autophagy and promotes viability in Hodgkin lymphoma: Implications for targeted therapy

Epstein‐Barr virus latent membrane protein‐1 upregulates autophagy and promotes viability in Hodgkin lymphoma: Implications for targeted therapy

Overexpression of interleukin-20 correlates with favourable prognosis in diffuse large B-cell lymphoma

The regulatory role of pioneer factors during cardiovascular lineage specification – A mini review

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