FGF signaling promotes myoblast proliferation through activation of wingless signaling

Vishal, Kumar; Lovato, TyAnna L.; Bragg, Chandler; Chechenova, Maria B.; Cripps, Richard M.

doi:10.1016/j.ydbio.2020.05.009

Cited by 14 publications

(24 citation statements)

References 55 publications

(66 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, the localized expression and level of Ths and Pyr secreted by epithelial cells in the notum could provide sufficient trophic support to generate the appropriate number of AMPs during normal development. While this work was in preparation, another group independently showed that the ths-Gal4 line is expressed in the notum epithelium and that reducing ths function reduces AMP numbers ( Vishal et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Single-cell transcriptomics of the Drosophila wing disc reveals instructive epithelium-to-myoblast interactions

Everetts

Worley

Yasutomi

et al. 2021

eLife

View full text Add to dashboard Cite

In both vertebrates and invertebrates, generating a functional appendage requires interactions between ectoderm-derived epithelia and mesoderm-derived cells. To investigate such interactions, we used single-cell transcriptomics to generate a temporal cell atlas of the Drosophila wing disc from two developmental time points. Using these data, we visualized gene expression using a multi-layered model of the wing disc and catalogued ligand-receptor pairs that could mediate signaling between epithelial cells and adult muscle precursors (AMPs). We found that localized expression of the FGF ligands, Thisbe and Pyramus, in the disc epithelium regulates the number and location of the AMPs. In addition, Hedgehog ligand from the epithelium activates a specific transcriptional program within adjacent AMP cells, defined by AMP-specific targets Neurotactin and midline, that is critical for proper formation of direct flight muscles. More generally, our annotated temporal cell atlas provides an organ-wide view of potential cell-cell interactions between epithelial and myogenic cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Single-cell transcriptomics of the Drosophila wing disc reveals instructive epithelium-to-myoblast interactions

Everetts

Worley

Yasutomi

et al. 2021

eLife

View full text Add to dashboard Cite

show abstract

“…Interestingly, previous studies found that FGF10 emanated from the prospective limb mesoderm in birds to serve as an endogenous initiator for limb bud formation, which is associated with the loss of ight in emu (Dromaius novaehollandiae) [43,44]. In drosophila, components of FGF signaling are expressed in myoblasts, a series of experiments show that FGF is a key factor of the development of ight muscle in drosophila [45,46]. Therefore, FGF14 very likely plays an important role in loss of ight in domesticated ducks.…”

Section: Discussionmentioning

confidence: 99%

Positive selection of skeleton genes during domestication indicates the potential genetic mechanism for loss of flight in ducks

Zhu

Yu³

et al. 2021

Preprint

View full text Add to dashboard Cite

Background Domestication alters lots of phenotypic, neurologic and physiologic traits between domestic animals and their wild ancestors. Domestic ducks were originated from mallards (Anas platyrhynchos) and some documents also showed that spot-billed ducks (Anas zonorhyncha) could also genetically contribute a small part to the domestication. Compared with the two ancestral species, domestic ducks generally present changes in body size and bone morphology, which is supposed to lead to loss of fight in domestic ducks. In the present study, we performed both genomic and transcriptomic analysis to identify candidate genes in order to elucidate the genetic mechanism underlying the phenotypic variation. Results Our results showed that genes associated with the skeleton systems were positively selected during domestication by Fst analysis between the wild and domestic ducks. We also found that many differentially expressed genes (DEGs) in the breast muscle between the wild and domestic ducks were enriched in the pathway for ossification. Among the genes, FGF14 and EIF2AK3 were also under strong selection by the genomic data, and they were both reported to be associated with limb morphology, bone development and flightlessness in some bird species. Conclusions Our study showed that the skeleton related genes were positively selected in the process of domestication, which could also cause the loss of flight in domestic ducks.

show abstract

“…In Drosophila, the wing disc epithelium releases FGF, which acts through Heartless (Htl) to activate the Wingless pathway. 79 This activation is required to achieve normal levels of AMP proliferation, and constitutively active Htl receptors result in an enlarged myoblast pool, at least in the wing disc.…”

Section: Metamorphosis In Insect Muscle 333mentioning

confidence: 99%

“…Previous work in this field has already demonstrated that transfection with a constitutively active form of the FGF receptor Heartless is known to increase AMP proliferation in vivo, and transfection with the Caenorhabditis elegans fusogenic protein Eff-1 can initiate fusion in a nonfusing cell line, especially with overexpression of Sns. 79,84 In addition, it might be possible to define factors controlling the identities of specific IPs and to use these cells to direct the eventual fate of the muscles as they develop in vitro.…”

Section: Strategies For Engineering Insect Musclementioning

confidence: 99%

Metamorphosis in Insect Muscle: Insights for Engineering Muscle-Based Actuators

Ludwig

Trimmer

2021

Tissue Engineering Part B: Reviews

View full text Add to dashboard Cite

One of the major limitations to advancing the development of soft robots is the absence of lightweight, effective soft actuators. While synthetic systems, such as pneumatics and shape memory alloys, have created important breakthroughs in soft actuation, they typically rely on large external power sources and some rigid components. Muscles provide an ideal actuator for soft constructs, as they are lightweight, deformable, biodegradable, silent, and powered by energy-dense hydrocarbons such as glucose. Vertebrate cell lines and embryonic cultures have allowed critical foundational work to this end, but progress there is limited by the difficulty of identifying individual pathways in embryonic development, and the divergence of immortal cell lines from these normal developmental programs. An alternative to culturing muscles from embryonic cells is to exploit the advantages of species with metamorphic stages. In these animals, muscles develop from a predefined pool of myoblasts with well-characterized contacts to other tissues. In addition, the endocrine triggers for development into adult muscles are often known and tractable for experimental manipulation. This is particularly true for metamorphic muscle development in holometabolous insects, which provide exciting new avenues for tissue engineering. Using insect tissues for actuator development confers additional benefits; insect muscles are more robust to varying pH, temperature, and oxygenation than are vertebrate cells. Given that biohybrid robots are likely to be used in ambient conditions and changing environments, this sort of hardiness is likely to be required for practical use. In this study, we summarize key processes and signals in metamorphic muscle development, drawing attention to those pathways that offer entry points for manipulation. By focusing on lessons learned from in vivo insect development, we propose that future culture designs will be able to use more systematic, hypothesisdriven approaches to optimizing engineered muscle.

show abstract

FGF signaling promotes myoblast proliferation through activation of wingless signaling

Cited by 14 publications

References 55 publications

Single-cell transcriptomics of the Drosophila wing disc reveals instructive epithelium-to-myoblast interactions

Single-cell transcriptomics of the Drosophila wing disc reveals instructive epithelium-to-myoblast interactions

Positive selection of skeleton genes during domestication indicates the potential genetic mechanism for loss of flight in ducks

Metamorphosis in Insect Muscle: Insights for Engineering Muscle-Based Actuators

Contact Info

Product

Resources

About