FGFR4 (fibroblast growth factor receptor 4)

Abstract

IdentityOther names: CD334, JTK2, TKF HGNC (Hugo): DNA/RNA DescriptionThe DNA was cloned in 1991 (Partanen et al., 1991). 18.9 kb; 18 exons. Transcription3.1 kb mRNA; alternative splicing gives rise to 18 transcripts with evidence of 11 transcripts at protein level.FGFR4 comprises an extracellular domain, a transmembrane domain, and an intracellular domain. FGFR4 alternative splicing has been described, with up to 18 different transcripts ranging from 552 to 3559 bp, (evidence of 11 transcripts at protein level ranging from 87 to 1030 aminoacids).

Electrochemical sensor for rapid determination of fibroblast growth factor receptor 4 in raw cancer cell lysates

“…Results displayed in Fig 4 show as similar S/B ratios were obtained in the absence and in the presence of TNFα, p53, BSA, and hemoglobin, thus indicating that no significant interference of these non-target proteins occurred in the FGFR4 determination. However, a dramatic reduction in the S/B ratio was observed in the presence of 1 mg mL -1 human IgG (bar 6 in Fig 4) which can be attributed to the presence of human anti-mouse antibodies (HAMAs) or to the three immunoglobulin-like domains of FGFR4 [24] which would lead to significant errors in sandwich immunoassays using mouse monoclonal antibodies, since they can cross-link the capture and labeled antibodies in the absence of the analyte [25]. However, it is important to mention that the quantification of the target receptor at a 2,500 pg mL -1 level was still feasible in the presence of such high concentration of human IgG.…”