fgr proto-oncogene mRNA induced in B lymphocytes by Epstein–Barr virus infection

Cheah, Marc S.C.; Ley, Timothy J.; Tronick, Steven R.; Robbins, K C

doi:10.1038/319238a0

Cited by 93 publications

(64 citation statements)

References 27 publications

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“…A similar pattern of transcripts has been described in the human Blymphocyte cell line IM-9, derived by in vitro infection of normal peripheral blood B-lymphocytes with EBV (Inoue et al, 1987). Cheah et al (1986) were unable to find c-fgr transcripts in the EBV-negative BL cell lines which they studied, but we find that low levels are detectable in the EBV-negative parent cell lines BL2, BL31 and BL41. Many of the changes in cellular phenotype which accompany EBVconversion resemble changes seen during normal Blymphocyte activation, most strikingly the acquisition of cell surface markers such as CD23 and Blast-I (Swendeman & Thorley-Lawson, 1987).…”

Section: Discussionmentioning

confidence: 61%

“…One candidate is the cellular protooncogene c-fgr, which is a member of a family of genes encoding intracellular proteins with tyrosine kinase activity . Transcripts of c-fgr are induced in B-lymphocytes immortalised by Epstein-Barr virus (EBV) and in EBV-negative Burkitt's lymphoma (BL) cell lines converted with EBV in vitro (Cheah et al, 1986). Both of these events bear many features in common with B-lymphocyte activation (Thorley-Lawson & Mann, 1985), involving changes in growth properties (Zeuthen, 1983) and in the expression of B-lymphocyte cell surface activation markers (Rowe et al, 1986).…”

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confidence: 99%

“…Both of these events bear many features in common with B-lymphocyte activation (Thorley-Lawson & Mann, 1985), involving changes in growth properties (Zeuthen, 1983) and in the expression of B-lymphocyte cell surface activation markers (Rowe et al, 1986). Cheah et al (1986) showed that EBV converts of the EBV-negative BL cell lines Ramos and BJAB contained a 3 kb transcript homologous to the cellular proto-oncogene c-fgr, which was undetectable in Ramos and BJAB themselves. Ramos and BJAB and their converts are longestablished cell lines, and have had the opportunity to accumulate phenotypic changes unrelated to their initial EBV conversion.…”

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confidence: 99%

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Structure and expression of c-fgr protooncogene mRNA in Epstein-Barr virus converted cell lines

Brickell

Patel²

1988

Br J Cancer

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Summary The c-fgr protooncogene is a member of the c-src family of tyrosine kinases. Expression of c-fgr was studied in a series of Epstein-Barr virus (EBV) negative Burkitt's lymphoma cell lines and their EBVconverted derivatives. Two transcripts, of 2.9kb and 3.5kb, were present at dramatically elevated levels following EBV-conversion.The structure of the c-fgr transcripts was studied by the isolation and nucleotide sequence analysis of cDNA clones. This indicated that the c-fgr protein encoded by the mature mRNA would contain 529 amino acids and have a molecular weight of approximately 58,000. The N-terminus of the predicted c-fgr protein has low amino acid homology with the N-termini of other members of this family of proteins, suggesting a cell specific function for the N-terminal domain. Analysis of the c-fgr cDNA clones also revealed the presence of alternative functional polyadenylation signals, although the use of these does not account for the size difference between the two major c-fgr transcripts.

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Section: Discussionmentioning

confidence: 61%

mentioning

confidence: 99%

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confidence: 99%

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Structure and expression of c-fgr protooncogene mRNA in Epstein-Barr virus converted cell lines

Brickell

Patel²

1988

Br J Cancer

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“…It has been reported that Fgr is expressed in large granular lymphocytes (LGL) in humans (7). LGL is thought to constitute the third population (non-T, non-B) of lymphocytes, natural killer (NK) cells and LAK cells.…”

Section: Lymphokine-activated Killer (Lak) Cells Express Fgrmentioning

confidence: 99%

“…On the other hand, it was shown that Hck and Fgr were specifically expressed in cells of monocyte/macrophage lineage (3). Expression of Fgr has been reported also in granulocytes (6), natural killer cells (20), and Epstein-Barr virus-infected B cells (7), but not in normal B cells in resting conditions. Thus, Fgr seems to be specifically expressed in cells involved in natural immunity.…”

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confidence: 99%

Fgr Expression Restricted to Subpopulation of Monocyte/Macrophage Lineage in Resting Conditions Is Induced in Various Hematopoietic Cells after Activation or Transformation

Hatakeyama

Iwabuchi

Ato

et al. 1996

Microbiology and Immunology

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The c‐fgr gene product (Fgr) is a member of the src‐family of protein tyrosine kinases. We have established a monoclonal antibody (2H2) which recognizes the unique N‐terminal domain of the murine Fgr. In the present study, using immunohistochemical analysis and immune complex kinase assay with the 2H2, we investigated expression of Fgr in various cell populations and tissues in a murine system. In resting conditions, Fgr expression was confined to subsets of a monocyte/macrophage lineage. Thus, Fgr+ cells were detected in paracortical areas and medullas of lymph nodes, but seen only in marginal zones of the spleen and the medulla of the thymus. No Fgr+ macrophage was detected in other tissues, Peyer's patches, brain, heart, lung, liver, pancreas, kidney and peritoneal cavity. However, immune complex kinase assay revealed that, upon stimulation, T and B cells as well as peritoneal macrophages expressed significant levels of Fgr molecules. Transformed cell lines of lymphoid origin, EL‐4 and LK35.2, which are T and B lineage lymphomas, respectively, also expressed Fgr molecules. Thus, various cells of hematopoietic origin appeared to possess a potentiality to express Fgr following activation or transformation. The present findings may help elucidate the functional significance of Fgr in immunologically committed cells in either activated or non‐activated conditions.

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Non‐receptor protein tyrosine kinases

Twamley

Courtneidge²

1996

Protein Phosphorylation

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fgr proto-oncogene mRNA induced in B lymphocytes by Epstein–Barr virus infection

Cited by 93 publications

References 27 publications

Structure and expression of c-fgr protooncogene mRNA in Epstein-Barr virus converted cell lines

Structure and expression of c-fgr protooncogene mRNA in Epstein-Barr virus converted cell lines

Fgr Expression Restricted to Subpopulation of Monocyte/Macrophage Lineage in Resting Conditions Is Induced in Various Hematopoietic Cells after Activation or Transformation

Non‐receptor protein tyrosine kinases

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